Job ID = 6458191 SRX = SRX495301 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:10:34 prefetch.2.10.7: 1) Downloading 'SRR1198806'... 2020-06-21T12:10:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:52 prefetch.2.10.7: 1) 'SRR1198806' was downloaded successfully Read 31586842 spots for SRR1198806/SRR1198806.sra Written 31586842 spots for SRR1198806/SRR1198806.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:07 31586842 reads; of these: 31586842 (100.00%) were unpaired; of these: 1523043 (4.82%) aligned 0 times 24360447 (77.12%) aligned exactly 1 time 5703352 (18.06%) aligned >1 times 95.18% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7322601 / 30063799 = 0.2436 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:50: 1000000 INFO @ Sun, 21 Jun 2020 21:32:56: 2000000 INFO @ Sun, 21 Jun 2020 21:33:02: 3000000 INFO @ Sun, 21 Jun 2020 21:33:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:13: 5000000 INFO @ Sun, 21 Jun 2020 21:33:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:20: 6000000 INFO @ Sun, 21 Jun 2020 21:33:21: 1000000 INFO @ Sun, 21 Jun 2020 21:33:27: 7000000 INFO @ Sun, 21 Jun 2020 21:33:28: 2000000 INFO @ Sun, 21 Jun 2020 21:33:34: 8000000 INFO @ Sun, 21 Jun 2020 21:33:35: 3000000 INFO @ Sun, 21 Jun 2020 21:33:41: 9000000 INFO @ Sun, 21 Jun 2020 21:33:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:47: 10000000 INFO @ Sun, 21 Jun 2020 21:33:48: 5000000 INFO @ Sun, 21 Jun 2020 21:33:51: 1000000 INFO @ Sun, 21 Jun 2020 21:33:54: 11000000 INFO @ Sun, 21 Jun 2020 21:33:55: 6000000 INFO @ Sun, 21 Jun 2020 21:33:58: 2000000 INFO @ Sun, 21 Jun 2020 21:34:01: 12000000 INFO @ Sun, 21 Jun 2020 21:34:02: 7000000 INFO @ Sun, 21 Jun 2020 21:34:05: 3000000 INFO @ Sun, 21 Jun 2020 21:34:08: 13000000 INFO @ Sun, 21 Jun 2020 21:34:09: 8000000 INFO @ Sun, 21 Jun 2020 21:34:12: 4000000 INFO @ Sun, 21 Jun 2020 21:34:15: 14000000 INFO @ Sun, 21 Jun 2020 21:34:16: 9000000 INFO @ Sun, 21 Jun 2020 21:34:18: 5000000 INFO @ Sun, 21 Jun 2020 21:34:22: 15000000 INFO @ Sun, 21 Jun 2020 21:34:23: 10000000 INFO @ Sun, 21 Jun 2020 21:34:25: 6000000 INFO @ Sun, 21 Jun 2020 21:34:29: 16000000 INFO @ Sun, 21 Jun 2020 21:34:30: 11000000 INFO @ Sun, 21 Jun 2020 21:34:32: 7000000 INFO @ Sun, 21 Jun 2020 21:34:35: 17000000 INFO @ Sun, 21 Jun 2020 21:34:36: 12000000 INFO @ Sun, 21 Jun 2020 21:34:39: 8000000 INFO @ Sun, 21 Jun 2020 21:34:42: 18000000 INFO @ Sun, 21 Jun 2020 21:34:43: 13000000 INFO @ Sun, 21 Jun 2020 21:34:46: 9000000 INFO @ Sun, 21 Jun 2020 21:34:49: 19000000 INFO @ Sun, 21 Jun 2020 21:34:50: 14000000 INFO @ Sun, 21 Jun 2020 21:34:53: 10000000 INFO @ Sun, 21 Jun 2020 21:34:56: 20000000 INFO @ Sun, 21 Jun 2020 21:34:57: 15000000 INFO @ Sun, 21 Jun 2020 21:35:00: 11000000 INFO @ Sun, 21 Jun 2020 21:35:03: 21000000 INFO @ Sun, 21 Jun 2020 21:35:04: 16000000 INFO @ Sun, 21 Jun 2020 21:35:06: 12000000 INFO @ Sun, 21 Jun 2020 21:35:10: 22000000 INFO @ Sun, 21 Jun 2020 21:35:11: 17000000 INFO @ Sun, 21 Jun 2020 21:35:13: 13000000 INFO @ Sun, 21 Jun 2020 21:35:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:35:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:35:15: #1 total tags in treatment: 22741198 INFO @ Sun, 21 Jun 2020 21:35:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:16: #1 tags after filtering in treatment: 22741191 INFO @ Sun, 21 Jun 2020 21:35:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:17: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 21:35:17: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:35:18: 18000000 INFO @ Sun, 21 Jun 2020 21:35:20: 14000000 INFO @ Sun, 21 Jun 2020 21:35:25: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:35:27: 15000000 INFO @ Sun, 21 Jun 2020 21:35:32: 20000000 INFO @ Sun, 21 Jun 2020 21:35:34: 16000000 INFO @ Sun, 21 Jun 2020 21:35:39: 21000000 INFO @ Sun, 21 Jun 2020 21:35:41: 17000000 INFO @ Sun, 21 Jun 2020 21:35:45: 22000000 INFO @ Sun, 21 Jun 2020 21:35:48: 18000000 INFO @ Sun, 21 Jun 2020 21:35:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:35:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:35:51: #1 total tags in treatment: 22741198 INFO @ Sun, 21 Jun 2020 21:35:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:51: #1 tags after filtering in treatment: 22741191 INFO @ Sun, 21 Jun 2020 21:35:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:53: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 21:35:53: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:35:54: 19000000 INFO @ Sun, 21 Jun 2020 21:36:01: 20000000 INFO @ Sun, 21 Jun 2020 21:36:07: 21000000 INFO @ Sun, 21 Jun 2020 21:36:13: 22000000 INFO @ Sun, 21 Jun 2020 21:36:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:36:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:36:17: #1 total tags in treatment: 22741198 INFO @ Sun, 21 Jun 2020 21:36:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:18: #1 tags after filtering in treatment: 22741191 INFO @ Sun, 21 Jun 2020 21:36:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:18: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:36:19: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 21:36:19: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495301/SRX495301.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling