Job ID = 6458190 SRX = SRX495300 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:29:34 prefetch.2.10.7: 1) Downloading 'SRR1198805'... 2020-06-21T12:29:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:34:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:34:36 prefetch.2.10.7: 1) 'SRR1198805' was downloaded successfully Read 31586842 spots for SRR1198805/SRR1198805.sra Written 31586842 spots for SRR1198805/SRR1198805.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:42 31586842 reads; of these: 31586842 (100.00%) were unpaired; of these: 1523163 (4.82%) aligned 0 times 24360372 (77.12%) aligned exactly 1 time 5703307 (18.06%) aligned >1 times 95.18% overall alignment rate Time searching: 00:08:43 Overall time: 00:08:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7322319 / 30063679 = 0.2436 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:42: 1000000 INFO @ Sun, 21 Jun 2020 21:52:50: 2000000 INFO @ Sun, 21 Jun 2020 21:52:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:05: 4000000 INFO @ Sun, 21 Jun 2020 21:53:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:06: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:06: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:14: 5000000 INFO @ Sun, 21 Jun 2020 21:53:15: 1000000 INFO @ Sun, 21 Jun 2020 21:53:23: 6000000 INFO @ Sun, 21 Jun 2020 21:53:24: 2000000 INFO @ Sun, 21 Jun 2020 21:53:32: 7000000 INFO @ Sun, 21 Jun 2020 21:53:32: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:41: 8000000 INFO @ Sun, 21 Jun 2020 21:53:41: 4000000 INFO @ Sun, 21 Jun 2020 21:53:43: 1000000 INFO @ Sun, 21 Jun 2020 21:53:49: 9000000 INFO @ Sun, 21 Jun 2020 21:53:50: 5000000 INFO @ Sun, 21 Jun 2020 21:53:51: 2000000 INFO @ Sun, 21 Jun 2020 21:53:58: 10000000 INFO @ Sun, 21 Jun 2020 21:53:59: 3000000 INFO @ Sun, 21 Jun 2020 21:53:59: 6000000 INFO @ Sun, 21 Jun 2020 21:54:07: 4000000 INFO @ Sun, 21 Jun 2020 21:54:08: 11000000 INFO @ Sun, 21 Jun 2020 21:54:08: 7000000 INFO @ Sun, 21 Jun 2020 21:54:15: 5000000 INFO @ Sun, 21 Jun 2020 21:54:16: 12000000 INFO @ Sun, 21 Jun 2020 21:54:17: 8000000 INFO @ Sun, 21 Jun 2020 21:54:22: 6000000 INFO @ Sun, 21 Jun 2020 21:54:25: 13000000 INFO @ Sun, 21 Jun 2020 21:54:26: 9000000 INFO @ Sun, 21 Jun 2020 21:54:30: 7000000 INFO @ Sun, 21 Jun 2020 21:54:34: 14000000 INFO @ Sun, 21 Jun 2020 21:54:35: 10000000 INFO @ Sun, 21 Jun 2020 21:54:38: 8000000 INFO @ Sun, 21 Jun 2020 21:54:43: 15000000 INFO @ Sun, 21 Jun 2020 21:54:44: 11000000 INFO @ Sun, 21 Jun 2020 21:54:46: 9000000 INFO @ Sun, 21 Jun 2020 21:54:52: 16000000 INFO @ Sun, 21 Jun 2020 21:54:53: 12000000 INFO @ Sun, 21 Jun 2020 21:54:54: 10000000 INFO @ Sun, 21 Jun 2020 21:55:01: 17000000 INFO @ Sun, 21 Jun 2020 21:55:02: 13000000 INFO @ Sun, 21 Jun 2020 21:55:02: 11000000 INFO @ Sun, 21 Jun 2020 21:55:10: 18000000 INFO @ Sun, 21 Jun 2020 21:55:10: 12000000 INFO @ Sun, 21 Jun 2020 21:55:11: 14000000 INFO @ Sun, 21 Jun 2020 21:55:18: 13000000 INFO @ Sun, 21 Jun 2020 21:55:19: 19000000 INFO @ Sun, 21 Jun 2020 21:55:20: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:55:26: 14000000 INFO @ Sun, 21 Jun 2020 21:55:27: 20000000 INFO @ Sun, 21 Jun 2020 21:55:29: 16000000 INFO @ Sun, 21 Jun 2020 21:55:33: 15000000 INFO @ Sun, 21 Jun 2020 21:55:36: 21000000 INFO @ Sun, 21 Jun 2020 21:55:37: 17000000 INFO @ Sun, 21 Jun 2020 21:55:41: 16000000 INFO @ Sun, 21 Jun 2020 21:55:44: 22000000 INFO @ Sun, 21 Jun 2020 21:55:46: 18000000 INFO @ Sun, 21 Jun 2020 21:55:49: 17000000 INFO @ Sun, 21 Jun 2020 21:55:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:51: #1 total tags in treatment: 22741360 INFO @ Sun, 21 Jun 2020 21:55:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:51: #1 tags after filtering in treatment: 22741351 INFO @ Sun, 21 Jun 2020 21:55:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:53: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:55:53: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:54: 19000000 INFO @ Sun, 21 Jun 2020 21:55:56: 18000000 INFO @ Sun, 21 Jun 2020 21:56:02: 20000000 INFO @ Sun, 21 Jun 2020 21:56:04: 19000000 INFO @ Sun, 21 Jun 2020 21:56:11: 21000000 INFO @ Sun, 21 Jun 2020 21:56:11: 20000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:56:18: 21000000 INFO @ Sun, 21 Jun 2020 21:56:19: 22000000 INFO @ Sun, 21 Jun 2020 21:56:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:25: #1 total tags in treatment: 22741360 INFO @ Sun, 21 Jun 2020 21:56:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:26: 22000000 INFO @ Sun, 21 Jun 2020 21:56:26: #1 tags after filtering in treatment: 22741351 INFO @ Sun, 21 Jun 2020 21:56:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:26: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:27: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:56:27: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:56:31: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:31: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:31: #1 total tags in treatment: 22741360 INFO @ Sun, 21 Jun 2020 21:56:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:32: #1 tags after filtering in treatment: 22741351 INFO @ Sun, 21 Jun 2020 21:56:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:32: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:33: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:56:33: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495300/SRX495300.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling