Job ID = 6508896 SRX = SRX495291 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:39:32 prefetch.2.10.7: 1) Downloading 'SRR1198796'... 2020-06-26T14:39:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:42:28 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:42:28 prefetch.2.10.7: 1) 'SRR1198796' was downloaded successfully Read 35336269 spots for SRR1198796/SRR1198796.sra Written 35336269 spots for SRR1198796/SRR1198796.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:04 35336269 reads; of these: 35336269 (100.00%) were unpaired; of these: 5685664 (16.09%) aligned 0 times 24061224 (68.09%) aligned exactly 1 time 5589381 (15.82%) aligned >1 times 83.91% overall alignment rate Time searching: 00:09:04 Overall time: 00:09:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7161697 / 29650605 = 0.2415 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:00:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:00:32: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:00:32: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:00:38: 1000000 INFO @ Sat, 27 Jun 2020 00:00:45: 2000000 INFO @ Sat, 27 Jun 2020 00:00:51: 3000000 INFO @ Sat, 27 Jun 2020 00:00:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:01:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:01:02: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:01:02: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:01:04: 5000000 INFO @ Sat, 27 Jun 2020 00:01:09: 1000000 INFO @ Sat, 27 Jun 2020 00:01:11: 6000000 INFO @ Sat, 27 Jun 2020 00:01:16: 2000000 INFO @ Sat, 27 Jun 2020 00:01:18: 7000000 INFO @ Sat, 27 Jun 2020 00:01:23: 3000000 INFO @ Sat, 27 Jun 2020 00:01:25: 8000000 INFO @ Sat, 27 Jun 2020 00:01:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:01:32: 9000000 INFO @ Sat, 27 Jun 2020 00:01:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:01:32: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:01:32: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:01:37: 5000000 INFO @ Sat, 27 Jun 2020 00:01:39: 10000000 INFO @ Sat, 27 Jun 2020 00:01:39: 1000000 INFO @ Sat, 27 Jun 2020 00:01:45: 6000000 INFO @ Sat, 27 Jun 2020 00:01:46: 11000000 INFO @ Sat, 27 Jun 2020 00:01:47: 2000000 INFO @ Sat, 27 Jun 2020 00:01:52: 7000000 INFO @ Sat, 27 Jun 2020 00:01:53: 12000000 INFO @ Sat, 27 Jun 2020 00:01:54: 3000000 INFO @ Sat, 27 Jun 2020 00:01:59: 8000000 INFO @ Sat, 27 Jun 2020 00:02:00: 13000000 INFO @ Sat, 27 Jun 2020 00:02:02: 4000000 INFO @ Sat, 27 Jun 2020 00:02:07: 14000000 INFO @ Sat, 27 Jun 2020 00:02:07: 9000000 INFO @ Sat, 27 Jun 2020 00:02:09: 5000000 INFO @ Sat, 27 Jun 2020 00:02:14: 15000000 INFO @ Sat, 27 Jun 2020 00:02:14: 10000000 INFO @ Sat, 27 Jun 2020 00:02:16: 6000000 INFO @ Sat, 27 Jun 2020 00:02:21: 16000000 INFO @ Sat, 27 Jun 2020 00:02:21: 11000000 INFO @ Sat, 27 Jun 2020 00:02:23: 7000000 INFO @ Sat, 27 Jun 2020 00:02:28: 17000000 INFO @ Sat, 27 Jun 2020 00:02:29: 12000000 INFO @ Sat, 27 Jun 2020 00:02:31: 8000000 INFO @ Sat, 27 Jun 2020 00:02:35: 18000000 INFO @ Sat, 27 Jun 2020 00:02:36: 13000000 INFO @ Sat, 27 Jun 2020 00:02:38: 9000000 INFO @ Sat, 27 Jun 2020 00:02:42: 19000000 INFO @ Sat, 27 Jun 2020 00:02:43: 14000000 INFO @ Sat, 27 Jun 2020 00:02:45: 10000000 INFO @ Sat, 27 Jun 2020 00:02:49: 20000000 INFO @ Sat, 27 Jun 2020 00:02:51: 15000000 INFO @ Sat, 27 Jun 2020 00:02:52: 11000000 INFO @ Sat, 27 Jun 2020 00:02:56: 21000000 INFO @ Sat, 27 Jun 2020 00:02:58: 16000000 INFO @ Sat, 27 Jun 2020 00:02:59: 12000000 INFO @ Sat, 27 Jun 2020 00:03:03: 22000000 INFO @ Sat, 27 Jun 2020 00:03:05: 17000000 INFO @ Sat, 27 Jun 2020 00:03:06: 13000000 INFO @ Sat, 27 Jun 2020 00:03:07: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:03:07: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:03:07: #1 total tags in treatment: 22488908 INFO @ Sat, 27 Jun 2020 00:03:07: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:03:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:03:08: #1 tags after filtering in treatment: 22488907 INFO @ Sat, 27 Jun 2020 00:03:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:03:08: #1 finished! INFO @ Sat, 27 Jun 2020 00:03:08: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:03:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:03:09: #2 number of paired peaks: 15 WARNING @ Sat, 27 Jun 2020 00:03:09: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:03:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:03:12: 18000000 INFO @ Sat, 27 Jun 2020 00:03:13: 14000000 INFO @ Sat, 27 Jun 2020 00:03:20: 19000000 INFO @ Sat, 27 Jun 2020 00:03:20: 15000000 INFO @ Sat, 27 Jun 2020 00:03:27: 20000000 INFO @ Sat, 27 Jun 2020 00:03:27: 16000000 INFO @ Sat, 27 Jun 2020 00:03:33: 21000000 INFO @ Sat, 27 Jun 2020 00:03:34: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:03:40: 22000000 INFO @ Sat, 27 Jun 2020 00:03:41: 18000000 INFO @ Sat, 27 Jun 2020 00:03:44: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:03:44: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:03:44: #1 total tags in treatment: 22488908 INFO @ Sat, 27 Jun 2020 00:03:44: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:03:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:03:45: #1 tags after filtering in treatment: 22488907 INFO @ Sat, 27 Jun 2020 00:03:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:03:45: #1 finished! INFO @ Sat, 27 Jun 2020 00:03:45: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:03:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:03:46: #2 number of paired peaks: 15 WARNING @ Sat, 27 Jun 2020 00:03:46: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:03:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:03:48: 19000000 INFO @ Sat, 27 Jun 2020 00:03:55: 20000000 INFO @ Sat, 27 Jun 2020 00:04:01: 21000000 INFO @ Sat, 27 Jun 2020 00:04:08: 22000000 INFO @ Sat, 27 Jun 2020 00:04:11: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:04:11: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:04:11: #1 total tags in treatment: 22488908 INFO @ Sat, 27 Jun 2020 00:04:11: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:04:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:04:12: #1 tags after filtering in treatment: 22488907 INFO @ Sat, 27 Jun 2020 00:04:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:04:12: #1 finished! INFO @ Sat, 27 Jun 2020 00:04:12: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:04:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:04:13: #2 number of paired peaks: 15 WARNING @ Sat, 27 Jun 2020 00:04:13: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:04:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495291/SRX495291.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。