Job ID = 6508888 SRX = SRX495283 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:42:19 prefetch.2.10.7: 1) Downloading 'SRR1198788'... 2020-06-26T14:42:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:46:09 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:46:09 prefetch.2.10.7: 1) 'SRR1198788' was downloaded successfully Read 55261147 spots for SRR1198788/SRR1198788.sra Written 55261147 spots for SRR1198788/SRR1198788.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:09 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1196386 (2.16%) aligned 0 times 45510230 (82.35%) aligned exactly 1 time 8554531 (15.48%) aligned >1 times 97.84% overall alignment rate Time searching: 00:14:09 Overall time: 00:14:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17942814 / 54064761 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:14:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:14:36: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:14:36: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:14:43: 1000000 INFO @ Sat, 27 Jun 2020 00:14:49: 2000000 INFO @ Sat, 27 Jun 2020 00:14:55: 3000000 INFO @ Sat, 27 Jun 2020 00:15:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:15:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:15:06: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:15:06: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:15:08: 5000000 INFO @ Sat, 27 Jun 2020 00:15:13: 1000000 INFO @ Sat, 27 Jun 2020 00:15:14: 6000000 INFO @ Sat, 27 Jun 2020 00:15:20: 2000000 INFO @ Sat, 27 Jun 2020 00:15:21: 7000000 INFO @ Sat, 27 Jun 2020 00:15:27: 3000000 INFO @ Sat, 27 Jun 2020 00:15:28: 8000000 INFO @ Sat, 27 Jun 2020 00:15:34: 4000000 INFO @ Sat, 27 Jun 2020 00:15:34: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:15:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:15:36: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:15:36: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:15:40: 5000000 INFO @ Sat, 27 Jun 2020 00:15:41: 10000000 INFO @ Sat, 27 Jun 2020 00:15:43: 1000000 INFO @ Sat, 27 Jun 2020 00:15:47: 6000000 INFO @ Sat, 27 Jun 2020 00:15:47: 11000000 INFO @ Sat, 27 Jun 2020 00:15:49: 2000000 INFO @ Sat, 27 Jun 2020 00:15:54: 12000000 INFO @ Sat, 27 Jun 2020 00:15:54: 7000000 INFO @ Sat, 27 Jun 2020 00:15:55: 3000000 INFO @ Sat, 27 Jun 2020 00:16:00: 13000000 INFO @ Sat, 27 Jun 2020 00:16:01: 4000000 INFO @ Sat, 27 Jun 2020 00:16:01: 8000000 INFO @ Sat, 27 Jun 2020 00:16:07: 14000000 INFO @ Sat, 27 Jun 2020 00:16:07: 5000000 INFO @ Sat, 27 Jun 2020 00:16:08: 9000000 INFO @ Sat, 27 Jun 2020 00:16:13: 6000000 INFO @ Sat, 27 Jun 2020 00:16:14: 15000000 INFO @ Sat, 27 Jun 2020 00:16:15: 10000000 INFO @ Sat, 27 Jun 2020 00:16:19: 7000000 INFO @ Sat, 27 Jun 2020 00:16:20: 16000000 INFO @ Sat, 27 Jun 2020 00:16:22: 11000000 INFO @ Sat, 27 Jun 2020 00:16:25: 8000000 INFO @ Sat, 27 Jun 2020 00:16:28: 17000000 INFO @ Sat, 27 Jun 2020 00:16:29: 12000000 INFO @ Sat, 27 Jun 2020 00:16:31: 9000000 INFO @ Sat, 27 Jun 2020 00:16:35: 18000000 INFO @ Sat, 27 Jun 2020 00:16:36: 13000000 INFO @ Sat, 27 Jun 2020 00:16:37: 10000000 INFO @ Sat, 27 Jun 2020 00:16:42: 19000000 INFO @ Sat, 27 Jun 2020 00:16:43: 14000000 INFO @ Sat, 27 Jun 2020 00:16:43: 11000000 INFO @ Sat, 27 Jun 2020 00:16:49: 20000000 INFO @ Sat, 27 Jun 2020 00:16:49: 12000000 INFO @ Sat, 27 Jun 2020 00:16:50: 15000000 INFO @ Sat, 27 Jun 2020 00:16:55: 13000000 INFO @ Sat, 27 Jun 2020 00:16:56: 21000000 INFO @ Sat, 27 Jun 2020 00:16:56: 16000000 INFO @ Sat, 27 Jun 2020 00:17:02: 14000000 INFO @ Sat, 27 Jun 2020 00:17:03: 22000000 INFO @ Sat, 27 Jun 2020 00:17:03: 17000000 INFO @ Sat, 27 Jun 2020 00:17:08: 15000000 INFO @ Sat, 27 Jun 2020 00:17:10: 23000000 INFO @ Sat, 27 Jun 2020 00:17:10: 18000000 INFO @ Sat, 27 Jun 2020 00:17:14: 16000000 INFO @ Sat, 27 Jun 2020 00:17:17: 19000000 INFO @ Sat, 27 Jun 2020 00:17:17: 24000000 INFO @ Sat, 27 Jun 2020 00:17:20: 17000000 INFO @ Sat, 27 Jun 2020 00:17:24: 20000000 INFO @ Sat, 27 Jun 2020 00:17:24: 25000000 INFO @ Sat, 27 Jun 2020 00:17:26: 18000000 INFO @ Sat, 27 Jun 2020 00:17:31: 21000000 INFO @ Sat, 27 Jun 2020 00:17:32: 26000000 INFO @ Sat, 27 Jun 2020 00:17:32: 19000000 INFO @ Sat, 27 Jun 2020 00:17:37: 22000000 INFO @ Sat, 27 Jun 2020 00:17:38: 20000000 INFO @ Sat, 27 Jun 2020 00:17:39: 27000000 INFO @ Sat, 27 Jun 2020 00:17:44: 23000000 INFO @ Sat, 27 Jun 2020 00:17:44: 21000000 INFO @ Sat, 27 Jun 2020 00:17:46: 28000000 INFO @ Sat, 27 Jun 2020 00:17:50: 22000000 INFO @ Sat, 27 Jun 2020 00:17:50: 24000000 INFO @ Sat, 27 Jun 2020 00:17:53: 29000000 INFO @ Sat, 27 Jun 2020 00:17:56: 23000000 INFO @ Sat, 27 Jun 2020 00:17:57: 25000000 INFO @ Sat, 27 Jun 2020 00:18:01: 30000000 INFO @ Sat, 27 Jun 2020 00:18:02: 24000000 INFO @ Sat, 27 Jun 2020 00:18:03: 26000000 INFO @ Sat, 27 Jun 2020 00:18:08: 31000000 INFO @ Sat, 27 Jun 2020 00:18:08: 25000000 INFO @ Sat, 27 Jun 2020 00:18:10: 27000000 INFO @ Sat, 27 Jun 2020 00:18:14: 26000000 INFO @ Sat, 27 Jun 2020 00:18:15: 32000000 INFO @ Sat, 27 Jun 2020 00:18:17: 28000000 INFO @ Sat, 27 Jun 2020 00:18:20: 27000000 INFO @ Sat, 27 Jun 2020 00:18:23: 33000000 INFO @ Sat, 27 Jun 2020 00:18:23: 29000000 INFO @ Sat, 27 Jun 2020 00:18:27: 28000000 INFO @ Sat, 27 Jun 2020 00:18:30: 34000000 INFO @ Sat, 27 Jun 2020 00:18:30: 30000000 INFO @ Sat, 27 Jun 2020 00:18:33: 29000000 INFO @ Sat, 27 Jun 2020 00:18:37: 31000000 INFO @ Sat, 27 Jun 2020 00:18:37: 35000000 INFO @ Sat, 27 Jun 2020 00:18:40: 30000000 INFO @ Sat, 27 Jun 2020 00:18:44: 32000000 INFO @ Sat, 27 Jun 2020 00:18:45: 36000000 INFO @ Sat, 27 Jun 2020 00:18:46: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:18:46: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:18:46: #1 total tags in treatment: 36121947 INFO @ Sat, 27 Jun 2020 00:18:46: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:18:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:18:46: 31000000 INFO @ Sat, 27 Jun 2020 00:18:47: #1 tags after filtering in treatment: 36121946 INFO @ Sat, 27 Jun 2020 00:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:18:47: #1 finished! INFO @ Sat, 27 Jun 2020 00:18:47: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:18:47: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:18:49: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:18:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:18:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:18:50: 33000000 INFO @ Sat, 27 Jun 2020 00:18:52: 32000000 INFO @ Sat, 27 Jun 2020 00:18:57: 34000000 INFO @ Sat, 27 Jun 2020 00:18:58: 33000000 INFO @ Sat, 27 Jun 2020 00:19:04: 35000000 INFO @ Sat, 27 Jun 2020 00:19:05: 34000000 INFO @ Sat, 27 Jun 2020 00:19:11: 36000000 INFO @ Sat, 27 Jun 2020 00:19:11: 35000000 INFO @ Sat, 27 Jun 2020 00:19:12: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:19:12: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:19:12: #1 total tags in treatment: 36121947 INFO @ Sat, 27 Jun 2020 00:19:12: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:19:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:19:13: #1 tags after filtering in treatment: 36121946 INFO @ Sat, 27 Jun 2020 00:19:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:19:13: #1 finished! INFO @ Sat, 27 Jun 2020 00:19:13: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:19:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:19:15: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:19:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:19:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:19:18: 36000000 INFO @ Sat, 27 Jun 2020 00:19:19: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:19:19: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:19:19: #1 total tags in treatment: 36121947 INFO @ Sat, 27 Jun 2020 00:19:19: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:19:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:19:20: #1 tags after filtering in treatment: 36121946 INFO @ Sat, 27 Jun 2020 00:19:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:19:20: #1 finished! INFO @ Sat, 27 Jun 2020 00:19:20: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:19:22: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:19:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:19:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495283/SRX495283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。