Job ID = 6508886 SRX = SRX495281 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:52:08 prefetch.2.10.7: 1) Downloading 'SRR1198786'... 2020-06-26T14:52:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:58:46 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:58:46 prefetch.2.10.7: 1) 'SRR1198786' was downloaded successfully Read 83972673 spots for SRR1198786/SRR1198786.sra Written 83972673 spots for SRR1198786/SRR1198786.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:17:25 83972673 reads; of these: 83972673 (100.00%) were unpaired; of these: 2675805 (3.19%) aligned 0 times 77647150 (92.47%) aligned exactly 1 time 3649718 (4.35%) aligned >1 times 96.81% overall alignment rate Time searching: 00:17:26 Overall time: 00:17:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 46853283 / 81296868 = 0.5763 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:38:46: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:38:46: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:38:52: 1000000 INFO @ Sat, 27 Jun 2020 00:38:58: 2000000 INFO @ Sat, 27 Jun 2020 00:39:05: 3000000 INFO @ Sat, 27 Jun 2020 00:39:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:39:16: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:39:16: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:39:18: 5000000 INFO @ Sat, 27 Jun 2020 00:39:23: 1000000 INFO @ Sat, 27 Jun 2020 00:39:25: 6000000 INFO @ Sat, 27 Jun 2020 00:39:30: 2000000 INFO @ Sat, 27 Jun 2020 00:39:32: 7000000 INFO @ Sat, 27 Jun 2020 00:39:37: 3000000 INFO @ Sat, 27 Jun 2020 00:39:40: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:39:44: 4000000 INFO @ Sat, 27 Jun 2020 00:39:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:39:46: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:39:46: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:39:47: 9000000 INFO @ Sat, 27 Jun 2020 00:39:52: 5000000 INFO @ Sat, 27 Jun 2020 00:39:53: 1000000 INFO @ Sat, 27 Jun 2020 00:39:54: 10000000 INFO @ Sat, 27 Jun 2020 00:39:59: 6000000 INFO @ Sat, 27 Jun 2020 00:40:01: 2000000 INFO @ Sat, 27 Jun 2020 00:40:02: 11000000 INFO @ Sat, 27 Jun 2020 00:40:07: 7000000 INFO @ Sat, 27 Jun 2020 00:40:09: 3000000 INFO @ Sat, 27 Jun 2020 00:40:09: 12000000 INFO @ Sat, 27 Jun 2020 00:40:14: 8000000 INFO @ Sat, 27 Jun 2020 00:40:16: 13000000 INFO @ Sat, 27 Jun 2020 00:40:17: 4000000 INFO @ Sat, 27 Jun 2020 00:40:21: 9000000 INFO @ Sat, 27 Jun 2020 00:40:24: 14000000 INFO @ Sat, 27 Jun 2020 00:40:25: 5000000 INFO @ Sat, 27 Jun 2020 00:40:29: 10000000 INFO @ Sat, 27 Jun 2020 00:40:31: 15000000 INFO @ Sat, 27 Jun 2020 00:40:32: 6000000 INFO @ Sat, 27 Jun 2020 00:40:36: 11000000 INFO @ Sat, 27 Jun 2020 00:40:39: 16000000 INFO @ Sat, 27 Jun 2020 00:40:40: 7000000 INFO @ Sat, 27 Jun 2020 00:40:43: 12000000 INFO @ Sat, 27 Jun 2020 00:40:46: 17000000 INFO @ Sat, 27 Jun 2020 00:40:48: 8000000 INFO @ Sat, 27 Jun 2020 00:40:51: 13000000 INFO @ Sat, 27 Jun 2020 00:40:53: 18000000 INFO @ Sat, 27 Jun 2020 00:40:56: 9000000 INFO @ Sat, 27 Jun 2020 00:40:58: 14000000 INFO @ Sat, 27 Jun 2020 00:41:01: 19000000 INFO @ Sat, 27 Jun 2020 00:41:04: 10000000 INFO @ Sat, 27 Jun 2020 00:41:06: 15000000 INFO @ Sat, 27 Jun 2020 00:41:08: 20000000 INFO @ Sat, 27 Jun 2020 00:41:12: 11000000 INFO @ Sat, 27 Jun 2020 00:41:13: 16000000 INFO @ Sat, 27 Jun 2020 00:41:15: 21000000 INFO @ Sat, 27 Jun 2020 00:41:20: 12000000 INFO @ Sat, 27 Jun 2020 00:41:20: 17000000 INFO @ Sat, 27 Jun 2020 00:41:22: 22000000 INFO @ Sat, 27 Jun 2020 00:41:27: 18000000 INFO @ Sat, 27 Jun 2020 00:41:27: 13000000 INFO @ Sat, 27 Jun 2020 00:41:29: 23000000 INFO @ Sat, 27 Jun 2020 00:41:35: 19000000 INFO @ Sat, 27 Jun 2020 00:41:35: 14000000 INFO @ Sat, 27 Jun 2020 00:41:37: 24000000 INFO @ Sat, 27 Jun 2020 00:41:42: 20000000 INFO @ Sat, 27 Jun 2020 00:41:43: 15000000 INFO @ Sat, 27 Jun 2020 00:41:44: 25000000 INFO @ Sat, 27 Jun 2020 00:41:50: 21000000 INFO @ Sat, 27 Jun 2020 00:41:51: 16000000 INFO @ Sat, 27 Jun 2020 00:41:51: 26000000 INFO @ Sat, 27 Jun 2020 00:41:57: 22000000 INFO @ Sat, 27 Jun 2020 00:41:59: 27000000 INFO @ Sat, 27 Jun 2020 00:41:59: 17000000 INFO @ Sat, 27 Jun 2020 00:42:04: 23000000 INFO @ Sat, 27 Jun 2020 00:42:06: 28000000 INFO @ Sat, 27 Jun 2020 00:42:07: 18000000 INFO @ Sat, 27 Jun 2020 00:42:11: 24000000 INFO @ Sat, 27 Jun 2020 00:42:14: 19000000 INFO @ Sat, 27 Jun 2020 00:42:14: 29000000 INFO @ Sat, 27 Jun 2020 00:42:18: 25000000 INFO @ Sat, 27 Jun 2020 00:42:22: 20000000 INFO @ Sat, 27 Jun 2020 00:42:24: 30000000 INFO @ Sat, 27 Jun 2020 00:42:25: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:42:31: 21000000 INFO @ Sat, 27 Jun 2020 00:42:32: 27000000 INFO @ Sat, 27 Jun 2020 00:42:33: 31000000 INFO @ Sat, 27 Jun 2020 00:42:38: 22000000 INFO @ Sat, 27 Jun 2020 00:42:40: 28000000 INFO @ Sat, 27 Jun 2020 00:42:41: 32000000 INFO @ Sat, 27 Jun 2020 00:42:46: 23000000 INFO @ Sat, 27 Jun 2020 00:42:48: 29000000 INFO @ Sat, 27 Jun 2020 00:42:48: 33000000 INFO @ Sat, 27 Jun 2020 00:42:54: 24000000 INFO @ Sat, 27 Jun 2020 00:42:55: 30000000 INFO @ Sat, 27 Jun 2020 00:42:56: 34000000 INFO @ Sat, 27 Jun 2020 00:43:00: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:43:00: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:43:00: #1 total tags in treatment: 34443585 INFO @ Sat, 27 Jun 2020 00:43:00: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:43:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:43:01: #1 tags after filtering in treatment: 34443531 INFO @ Sat, 27 Jun 2020 00:43:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:43:01: #1 finished! INFO @ Sat, 27 Jun 2020 00:43:01: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:43:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:43:01: 25000000 INFO @ Sat, 27 Jun 2020 00:43:03: 31000000 INFO @ Sat, 27 Jun 2020 00:43:03: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:43:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:43:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:43:09: 26000000 INFO @ Sat, 27 Jun 2020 00:43:09: 32000000 INFO @ Sat, 27 Jun 2020 00:43:16: 33000000 INFO @ Sat, 27 Jun 2020 00:43:16: 27000000 INFO @ Sat, 27 Jun 2020 00:43:23: 34000000 INFO @ Sat, 27 Jun 2020 00:43:25: 28000000 INFO @ Sat, 27 Jun 2020 00:43:27: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:43:27: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:43:27: #1 total tags in treatment: 34443585 INFO @ Sat, 27 Jun 2020 00:43:27: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:43:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:43:28: #1 tags after filtering in treatment: 34443531 INFO @ Sat, 27 Jun 2020 00:43:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:43:28: #1 finished! INFO @ Sat, 27 Jun 2020 00:43:28: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:43:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:43:30: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:43:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:43:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:43:33: 29000000 INFO @ Sat, 27 Jun 2020 00:43:40: 30000000 INFO @ Sat, 27 Jun 2020 00:43:48: 31000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:43:55: 32000000 INFO @ Sat, 27 Jun 2020 00:44:02: 33000000 INFO @ Sat, 27 Jun 2020 00:44:09: 34000000 INFO @ Sat, 27 Jun 2020 00:44:13: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:44:13: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:44:13: #1 total tags in treatment: 34443585 INFO @ Sat, 27 Jun 2020 00:44:13: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:44:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:44:14: #1 tags after filtering in treatment: 34443531 INFO @ Sat, 27 Jun 2020 00:44:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:44:14: #1 finished! INFO @ Sat, 27 Jun 2020 00:44:14: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:44:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:44:16: #2 number of paired peaks: 0 WARNING @ Sat, 27 Jun 2020 00:44:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:44:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495281/SRX495281.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling