Job ID = 6458155 SRX = SRX495271 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:16:19 prefetch.2.10.7: 1) Downloading 'SRR1198776'... 2020-06-21T12:16:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:27:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:27:35 prefetch.2.10.7: 1) 'SRR1198776' was downloaded successfully Read 84993195 spots for SRR1198776/SRR1198776.sra Written 84993195 spots for SRR1198776/SRR1198776.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:31 84993195 reads; of these: 84993195 (100.00%) were unpaired; of these: 3426724 (4.03%) aligned 0 times 63019585 (74.15%) aligned exactly 1 time 18546886 (21.82%) aligned >1 times 95.97% overall alignment rate Time searching: 00:21:31 Overall time: 00:21:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 36540233 / 81566471 = 0.4480 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:07:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:07:37: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:07:37: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:07:43: 1000000 INFO @ Sun, 21 Jun 2020 22:07:48: 2000000 INFO @ Sun, 21 Jun 2020 22:07:53: 3000000 INFO @ Sun, 21 Jun 2020 22:07:59: 4000000 INFO @ Sun, 21 Jun 2020 22:08:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:10: 6000000 INFO @ Sun, 21 Jun 2020 22:08:12: 1000000 INFO @ Sun, 21 Jun 2020 22:08:15: 7000000 INFO @ Sun, 21 Jun 2020 22:08:17: 2000000 INFO @ Sun, 21 Jun 2020 22:08:21: 8000000 INFO @ Sun, 21 Jun 2020 22:08:22: 3000000 INFO @ Sun, 21 Jun 2020 22:08:26: 9000000 INFO @ Sun, 21 Jun 2020 22:08:27: 4000000 INFO @ Sun, 21 Jun 2020 22:08:32: 10000000 INFO @ Sun, 21 Jun 2020 22:08:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:37: 6000000 INFO @ Sun, 21 Jun 2020 22:08:37: 11000000 INFO @ Sun, 21 Jun 2020 22:08:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:37: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:37: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:41: 7000000 INFO @ Sun, 21 Jun 2020 22:08:42: 1000000 INFO @ Sun, 21 Jun 2020 22:08:42: 12000000 INFO @ Sun, 21 Jun 2020 22:08:46: 8000000 INFO @ Sun, 21 Jun 2020 22:08:47: 2000000 INFO @ Sun, 21 Jun 2020 22:08:48: 13000000 INFO @ Sun, 21 Jun 2020 22:08:51: 9000000 INFO @ Sun, 21 Jun 2020 22:08:52: 3000000 INFO @ Sun, 21 Jun 2020 22:08:53: 14000000 INFO @ Sun, 21 Jun 2020 22:08:56: 10000000 INFO @ Sun, 21 Jun 2020 22:08:57: 4000000 INFO @ Sun, 21 Jun 2020 22:08:59: 15000000 INFO @ Sun, 21 Jun 2020 22:09:01: 11000000 INFO @ Sun, 21 Jun 2020 22:09:02: 5000000 INFO @ Sun, 21 Jun 2020 22:09:05: 16000000 INFO @ Sun, 21 Jun 2020 22:09:06: 12000000 INFO @ Sun, 21 Jun 2020 22:09:07: 6000000 INFO @ Sun, 21 Jun 2020 22:09:10: 13000000 INFO @ Sun, 21 Jun 2020 22:09:10: 17000000 INFO @ Sun, 21 Jun 2020 22:09:12: 7000000 INFO @ Sun, 21 Jun 2020 22:09:15: 14000000 INFO @ Sun, 21 Jun 2020 22:09:16: 18000000 INFO @ Sun, 21 Jun 2020 22:09:17: 8000000 INFO @ Sun, 21 Jun 2020 22:09:20: 15000000 INFO @ Sun, 21 Jun 2020 22:09:22: 9000000 INFO @ Sun, 21 Jun 2020 22:09:22: 19000000 INFO @ Sun, 21 Jun 2020 22:09:25: 16000000 INFO @ Sun, 21 Jun 2020 22:09:27: 10000000 INFO @ Sun, 21 Jun 2020 22:09:28: 20000000 INFO @ Sun, 21 Jun 2020 22:09:30: 17000000 INFO @ Sun, 21 Jun 2020 22:09:31: 11000000 INFO @ Sun, 21 Jun 2020 22:09:33: 21000000 INFO @ Sun, 21 Jun 2020 22:09:35: 18000000 INFO @ Sun, 21 Jun 2020 22:09:36: 12000000 INFO @ Sun, 21 Jun 2020 22:09:39: 22000000 INFO @ Sun, 21 Jun 2020 22:09:40: 19000000 INFO @ Sun, 21 Jun 2020 22:09:41: 13000000 INFO @ Sun, 21 Jun 2020 22:09:45: 20000000 INFO @ Sun, 21 Jun 2020 22:09:45: 23000000 INFO @ Sun, 21 Jun 2020 22:09:46: 14000000 INFO @ Sun, 21 Jun 2020 22:09:50: 21000000 INFO @ Sun, 21 Jun 2020 22:09:50: 24000000 INFO @ Sun, 21 Jun 2020 22:09:51: 15000000 INFO @ Sun, 21 Jun 2020 22:09:55: 22000000 INFO @ Sun, 21 Jun 2020 22:09:56: 16000000 INFO @ Sun, 21 Jun 2020 22:09:56: 25000000 INFO @ Sun, 21 Jun 2020 22:10:00: 23000000 INFO @ Sun, 21 Jun 2020 22:10:01: 17000000 INFO @ Sun, 21 Jun 2020 22:10:02: 26000000 INFO @ Sun, 21 Jun 2020 22:10:04: 24000000 INFO @ Sun, 21 Jun 2020 22:10:06: 18000000 INFO @ Sun, 21 Jun 2020 22:10:07: 27000000 INFO @ Sun, 21 Jun 2020 22:10:09: 25000000 INFO @ Sun, 21 Jun 2020 22:10:11: 19000000 INFO @ Sun, 21 Jun 2020 22:10:13: 28000000 INFO @ Sun, 21 Jun 2020 22:10:14: 26000000 INFO @ Sun, 21 Jun 2020 22:10:16: 20000000 INFO @ Sun, 21 Jun 2020 22:10:18: 29000000 INFO @ Sun, 21 Jun 2020 22:10:19: 27000000 INFO @ Sun, 21 Jun 2020 22:10:21: 21000000 INFO @ Sun, 21 Jun 2020 22:10:24: 28000000 INFO @ Sun, 21 Jun 2020 22:10:24: 30000000 INFO @ Sun, 21 Jun 2020 22:10:26: 22000000 INFO @ Sun, 21 Jun 2020 22:10:29: 29000000 INFO @ Sun, 21 Jun 2020 22:10:30: 31000000 INFO @ Sun, 21 Jun 2020 22:10:31: 23000000 INFO @ Sun, 21 Jun 2020 22:10:34: 30000000 INFO @ Sun, 21 Jun 2020 22:10:35: 32000000 INFO @ Sun, 21 Jun 2020 22:10:35: 24000000 INFO @ Sun, 21 Jun 2020 22:10:39: 31000000 INFO @ Sun, 21 Jun 2020 22:10:40: 25000000 INFO @ Sun, 21 Jun 2020 22:10:41: 33000000 INFO @ Sun, 21 Jun 2020 22:10:44: 32000000 INFO @ Sun, 21 Jun 2020 22:10:45: 26000000 INFO @ Sun, 21 Jun 2020 22:10:46: 34000000 INFO @ Sun, 21 Jun 2020 22:10:49: 33000000 INFO @ Sun, 21 Jun 2020 22:10:50: 27000000 INFO @ Sun, 21 Jun 2020 22:10:51: 35000000 INFO @ Sun, 21 Jun 2020 22:10:53: 34000000 INFO @ Sun, 21 Jun 2020 22:10:55: 28000000 INFO @ Sun, 21 Jun 2020 22:10:57: 36000000 INFO @ Sun, 21 Jun 2020 22:10:58: 35000000 INFO @ Sun, 21 Jun 2020 22:11:00: 29000000 INFO @ Sun, 21 Jun 2020 22:11:03: 37000000 INFO @ Sun, 21 Jun 2020 22:11:03: 36000000 INFO @ Sun, 21 Jun 2020 22:11:05: 30000000 INFO @ Sun, 21 Jun 2020 22:11:08: 38000000 INFO @ Sun, 21 Jun 2020 22:11:08: 37000000 INFO @ Sun, 21 Jun 2020 22:11:10: 31000000 INFO @ Sun, 21 Jun 2020 22:11:13: 38000000 INFO @ Sun, 21 Jun 2020 22:11:14: 39000000 INFO @ Sun, 21 Jun 2020 22:11:15: 32000000 INFO @ Sun, 21 Jun 2020 22:11:18: 39000000 INFO @ Sun, 21 Jun 2020 22:11:19: 40000000 INFO @ Sun, 21 Jun 2020 22:11:20: 33000000 INFO @ Sun, 21 Jun 2020 22:11:23: 40000000 INFO @ Sun, 21 Jun 2020 22:11:25: 41000000 INFO @ Sun, 21 Jun 2020 22:11:25: 34000000 INFO @ Sun, 21 Jun 2020 22:11:28: 41000000 INFO @ Sun, 21 Jun 2020 22:11:29: 35000000 INFO @ Sun, 21 Jun 2020 22:11:30: 42000000 INFO @ Sun, 21 Jun 2020 22:11:33: 42000000 INFO @ Sun, 21 Jun 2020 22:11:34: 36000000 INFO @ Sun, 21 Jun 2020 22:11:36: 43000000 INFO @ Sun, 21 Jun 2020 22:11:37: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:11:40: 37000000 INFO @ Sun, 21 Jun 2020 22:11:41: 44000000 INFO @ Sun, 21 Jun 2020 22:11:42: 44000000 INFO @ Sun, 21 Jun 2020 22:11:45: 38000000 INFO @ Sun, 21 Jun 2020 22:11:47: 45000000 INFO @ Sun, 21 Jun 2020 22:11:47: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:11:47: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:11:47: #1 total tags in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:11:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:11:48: 45000000 INFO @ Sun, 21 Jun 2020 22:11:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:11:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:11:48: #1 total tags in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:11:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:11:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:11:48: #1 tags after filtering in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:11:48: #1 finished! INFO @ Sun, 21 Jun 2020 22:11:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:11:49: #1 tags after filtering in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:11:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:11:49: #1 finished! INFO @ Sun, 21 Jun 2020 22:11:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:11:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:11:49: 39000000 INFO @ Sun, 21 Jun 2020 22:11:51: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:11:51: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:11:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:11:52: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:11:52: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:11:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:11:54: 40000000 INFO @ Sun, 21 Jun 2020 22:11:59: 41000000 INFO @ Sun, 21 Jun 2020 22:12:03: 42000000 INFO @ Sun, 21 Jun 2020 22:12:08: 43000000 INFO @ Sun, 21 Jun 2020 22:12:13: 44000000 INFO @ Sun, 21 Jun 2020 22:12:18: 45000000 INFO @ Sun, 21 Jun 2020 22:12:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:12:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:12:19: #1 total tags in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:12:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:12:19: #1 tags after filtering in treatment: 45026238 INFO @ Sun, 21 Jun 2020 22:12:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:12:19: #1 finished! INFO @ Sun, 21 Jun 2020 22:12:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:12:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:12:22: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:12:22: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:12:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495271/SRX495271.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。