Job ID = 6458146 SRX = SRX495263 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:39:56 prefetch.2.10.7: 1) Downloading 'SRR1198768'... 2020-06-21T12:39:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:42:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:42:25 prefetch.2.10.7: 1) 'SRR1198768' was downloaded successfully Read 24355341 spots for SRR1198768/SRR1198768.sra Written 24355341 spots for SRR1198768/SRR1198768.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:24 24355341 reads; of these: 24355341 (100.00%) were unpaired; of these: 1544287 (6.34%) aligned 0 times 20105213 (82.55%) aligned exactly 1 time 2705841 (11.11%) aligned >1 times 93.66% overall alignment rate Time searching: 00:05:24 Overall time: 00:05:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5059320 / 22811054 = 0.2218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:54:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:54:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:54:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:54:09: 1000000 INFO @ Sun, 21 Jun 2020 21:54:14: 2000000 INFO @ Sun, 21 Jun 2020 21:54:19: 3000000 INFO @ Sun, 21 Jun 2020 21:54:24: 4000000 INFO @ Sun, 21 Jun 2020 21:54:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:54:34: 6000000 INFO @ Sun, 21 Jun 2020 21:54:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:54:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:54:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:54:39: 7000000 INFO @ Sun, 21 Jun 2020 21:54:39: 1000000 INFO @ Sun, 21 Jun 2020 21:54:44: 8000000 INFO @ Sun, 21 Jun 2020 21:54:45: 2000000 INFO @ Sun, 21 Jun 2020 21:54:49: 9000000 INFO @ Sun, 21 Jun 2020 21:54:50: 3000000 INFO @ Sun, 21 Jun 2020 21:54:54: 10000000 INFO @ Sun, 21 Jun 2020 21:54:55: 4000000 INFO @ Sun, 21 Jun 2020 21:54:59: 11000000 INFO @ Sun, 21 Jun 2020 21:55:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:55:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:55:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:55:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:55:05: 12000000 INFO @ Sun, 21 Jun 2020 21:55:05: 6000000 INFO @ Sun, 21 Jun 2020 21:55:09: 1000000 INFO @ Sun, 21 Jun 2020 21:55:10: 13000000 INFO @ Sun, 21 Jun 2020 21:55:10: 7000000 INFO @ Sun, 21 Jun 2020 21:55:15: 2000000 INFO @ Sun, 21 Jun 2020 21:55:15: 14000000 INFO @ Sun, 21 Jun 2020 21:55:16: 8000000 INFO @ Sun, 21 Jun 2020 21:55:20: 3000000 INFO @ Sun, 21 Jun 2020 21:55:21: 15000000 INFO @ Sun, 21 Jun 2020 21:55:21: 9000000 INFO @ Sun, 21 Jun 2020 21:55:25: 4000000 INFO @ Sun, 21 Jun 2020 21:55:26: 10000000 INFO @ Sun, 21 Jun 2020 21:55:26: 16000000 INFO @ Sun, 21 Jun 2020 21:55:30: 5000000 INFO @ Sun, 21 Jun 2020 21:55:31: 11000000 INFO @ Sun, 21 Jun 2020 21:55:31: 17000000 INFO @ Sun, 21 Jun 2020 21:55:35: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:35: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:35: #1 total tags in treatment: 17751734 INFO @ Sun, 21 Jun 2020 21:55:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:35: 6000000 INFO @ Sun, 21 Jun 2020 21:55:36: #1 tags after filtering in treatment: 17751727 INFO @ Sun, 21 Jun 2020 21:55:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:36: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:36: 12000000 INFO @ Sun, 21 Jun 2020 21:55:37: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 21:55:37: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:41: 7000000 INFO @ Sun, 21 Jun 2020 21:55:41: 13000000 INFO @ Sun, 21 Jun 2020 21:55:46: 8000000 INFO @ Sun, 21 Jun 2020 21:55:46: 14000000 INFO @ Sun, 21 Jun 2020 21:55:51: 9000000 INFO @ Sun, 21 Jun 2020 21:55:52: 15000000 INFO @ Sun, 21 Jun 2020 21:55:56: 10000000 INFO @ Sun, 21 Jun 2020 21:55:57: 16000000 INFO @ Sun, 21 Jun 2020 21:56:01: 11000000 INFO @ Sun, 21 Jun 2020 21:56:02: 17000000 INFO @ Sun, 21 Jun 2020 21:56:06: 12000000 INFO @ Sun, 21 Jun 2020 21:56:06: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:06: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:06: #1 total tags in treatment: 17751734 INFO @ Sun, 21 Jun 2020 21:56:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:07: #1 tags after filtering in treatment: 17751727 INFO @ Sun, 21 Jun 2020 21:56:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:07: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:08: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 21:56:08: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10_peaks.narrowPeak: No such file or directory INFO @ Sun, 21 Jun 2020 21:56:11: 13000000 INFO @ Sun, 21 Jun 2020 21:56:16: 14000000 INFO @ Sun, 21 Jun 2020 21:56:22: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:56:27: 16000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:56:31: 17000000 INFO @ Sun, 21 Jun 2020 21:56:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:36: #1 total tags in treatment: 17751734 INFO @ Sun, 21 Jun 2020 21:56:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:36: #1 tags after filtering in treatment: 17751727 INFO @ Sun, 21 Jun 2020 21:56:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:36: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:37: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 21:56:37: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495263/SRX495263.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。