Job ID = 6458145 SRX = SRX495262 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:13:49 prefetch.2.10.7: 1) Downloading 'SRR1198767'... 2020-06-21T12:13:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:16:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:16:26 prefetch.2.10.7: 1) 'SRR1198767' was downloaded successfully Read 22615922 spots for SRR1198767/SRR1198767.sra Written 22615922 spots for SRR1198767/SRR1198767.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:56 22615922 reads; of these: 22615922 (100.00%) were unpaired; of these: 2126594 (9.40%) aligned 0 times 17771849 (78.58%) aligned exactly 1 time 2717479 (12.02%) aligned >1 times 90.60% overall alignment rate Time searching: 00:04:56 Overall time: 00:04:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4763505 / 20489328 = 0.2325 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:07: 1000000 INFO @ Sun, 21 Jun 2020 21:27:12: 2000000 INFO @ Sun, 21 Jun 2020 21:27:17: 3000000 INFO @ Sun, 21 Jun 2020 21:27:21: 4000000 INFO @ Sun, 21 Jun 2020 21:27:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:31: 6000000 INFO @ Sun, 21 Jun 2020 21:27:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:36: 7000000 INFO @ Sun, 21 Jun 2020 21:27:38: 1000000 INFO @ Sun, 21 Jun 2020 21:27:41: 8000000 INFO @ Sun, 21 Jun 2020 21:27:44: 2000000 INFO @ Sun, 21 Jun 2020 21:27:46: 9000000 INFO @ Sun, 21 Jun 2020 21:27:49: 3000000 INFO @ Sun, 21 Jun 2020 21:27:51: 10000000 INFO @ Sun, 21 Jun 2020 21:27:55: 4000000 INFO @ Sun, 21 Jun 2020 21:27:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:01: 5000000 INFO @ Sun, 21 Jun 2020 21:28:01: 12000000 INFO @ Sun, 21 Jun 2020 21:28:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:28:06: 6000000 INFO @ Sun, 21 Jun 2020 21:28:07: 13000000 INFO @ Sun, 21 Jun 2020 21:28:08: 1000000 INFO @ Sun, 21 Jun 2020 21:28:12: 14000000 INFO @ Sun, 21 Jun 2020 21:28:12: 7000000 INFO @ Sun, 21 Jun 2020 21:28:13: 2000000 INFO @ Sun, 21 Jun 2020 21:28:17: 15000000 INFO @ Sun, 21 Jun 2020 21:28:18: 3000000 INFO @ Sun, 21 Jun 2020 21:28:18: 8000000 INFO @ Sun, 21 Jun 2020 21:28:21: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:28:21: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:28:21: #1 total tags in treatment: 15725823 INFO @ Sun, 21 Jun 2020 21:28:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:28:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:28:21: #1 tags after filtering in treatment: 15725817 INFO @ Sun, 21 Jun 2020 21:28:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:28:21: #1 finished! INFO @ Sun, 21 Jun 2020 21:28:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:28:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:28:22: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 21:28:22: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:28:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:28:23: 4000000 INFO @ Sun, 21 Jun 2020 21:28:24: 9000000 INFO @ Sun, 21 Jun 2020 21:28:28: 5000000 INFO @ Sun, 21 Jun 2020 21:28:29: 10000000 INFO @ Sun, 21 Jun 2020 21:28:33: 6000000 INFO @ Sun, 21 Jun 2020 21:28:35: 11000000 INFO @ Sun, 21 Jun 2020 21:28:38: 7000000 INFO @ Sun, 21 Jun 2020 21:28:41: 12000000 INFO @ Sun, 21 Jun 2020 21:28:43: 8000000 INFO @ Sun, 21 Jun 2020 21:28:47: 13000000 INFO @ Sun, 21 Jun 2020 21:28:48: 9000000 INFO @ Sun, 21 Jun 2020 21:28:52: 14000000 INFO @ Sun, 21 Jun 2020 21:28:54: 10000000 INFO @ Sun, 21 Jun 2020 21:28:58: 15000000 INFO @ Sun, 21 Jun 2020 21:28:59: 11000000 INFO @ Sun, 21 Jun 2020 21:29:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:29:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:29:03: #1 total tags in treatment: 15725823 INFO @ Sun, 21 Jun 2020 21:29:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:03: #1 tags after filtering in treatment: 15725817 INFO @ Sun, 21 Jun 2020 21:29:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:03: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:04: 12000000 INFO @ Sun, 21 Jun 2020 21:29:04: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 21:29:04: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:29:09: 13000000 INFO @ Sun, 21 Jun 2020 21:29:14: 14000000 INFO @ Sun, 21 Jun 2020 21:29:19: 15000000 INFO @ Sun, 21 Jun 2020 21:29:23: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:29:23: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:29:23: #1 total tags in treatment: 15725823 INFO @ Sun, 21 Jun 2020 21:29:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:24: #1 tags after filtering in treatment: 15725817 INFO @ Sun, 21 Jun 2020 21:29:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:24: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:25: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 21:29:25: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495262/SRX495262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。