Job ID = 6458144 SRX = SRX495261 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:08:51 prefetch.2.10.7: 1) Downloading 'SRR1198766'... 2020-06-21T12:08:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:13:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:13:09 prefetch.2.10.7: 1) 'SRR1198766' was downloaded successfully Read 23779885 spots for SRR1198766/SRR1198766.sra Written 23779885 spots for SRR1198766/SRR1198766.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:58 23779885 reads; of these: 23779885 (100.00%) were unpaired; of these: 2785315 (11.71%) aligned 0 times 19149825 (80.53%) aligned exactly 1 time 1844745 (7.76%) aligned >1 times 88.29% overall alignment rate Time searching: 00:04:58 Overall time: 00:04:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4939849 / 20994570 = 0.2353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:24:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:24:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:24:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:08: 1000000 INFO @ Sun, 21 Jun 2020 21:24:13: 2000000 INFO @ Sun, 21 Jun 2020 21:24:19: 3000000 INFO @ Sun, 21 Jun 2020 21:24:24: 4000000 INFO @ Sun, 21 Jun 2020 21:24:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:24:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:24:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:24:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:35: 6000000 INFO @ Sun, 21 Jun 2020 21:24:39: 1000000 INFO @ Sun, 21 Jun 2020 21:24:42: 7000000 INFO @ Sun, 21 Jun 2020 21:24:45: 2000000 INFO @ Sun, 21 Jun 2020 21:24:48: 8000000 INFO @ Sun, 21 Jun 2020 21:24:52: 3000000 INFO @ Sun, 21 Jun 2020 21:24:54: 9000000 INFO @ Sun, 21 Jun 2020 21:24:59: 4000000 INFO @ Sun, 21 Jun 2020 21:25:00: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:25:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:25:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:25:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:25:06: 5000000 INFO @ Sun, 21 Jun 2020 21:25:06: 11000000 INFO @ Sun, 21 Jun 2020 21:25:09: 1000000 INFO @ Sun, 21 Jun 2020 21:25:12: 12000000 INFO @ Sun, 21 Jun 2020 21:25:12: 6000000 INFO @ Sun, 21 Jun 2020 21:25:16: 2000000 INFO @ Sun, 21 Jun 2020 21:25:18: 13000000 INFO @ Sun, 21 Jun 2020 21:25:19: 7000000 INFO @ Sun, 21 Jun 2020 21:25:23: 3000000 INFO @ Sun, 21 Jun 2020 21:25:25: 14000000 INFO @ Sun, 21 Jun 2020 21:25:26: 8000000 INFO @ Sun, 21 Jun 2020 21:25:30: 4000000 INFO @ Sun, 21 Jun 2020 21:25:32: 15000000 INFO @ Sun, 21 Jun 2020 21:25:32: 9000000 INFO @ Sun, 21 Jun 2020 21:25:37: 5000000 INFO @ Sun, 21 Jun 2020 21:25:38: 16000000 INFO @ Sun, 21 Jun 2020 21:25:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:25:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:25:38: #1 total tags in treatment: 16054721 INFO @ Sun, 21 Jun 2020 21:25:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:39: 10000000 INFO @ Sun, 21 Jun 2020 21:25:39: #1 tags after filtering in treatment: 16054673 INFO @ Sun, 21 Jun 2020 21:25:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:40: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:25:40: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:25:44: 6000000 INFO @ Sun, 21 Jun 2020 21:25:45: 11000000 INFO @ Sun, 21 Jun 2020 21:25:51: 7000000 INFO @ Sun, 21 Jun 2020 21:25:52: 12000000 INFO @ Sun, 21 Jun 2020 21:25:58: 8000000 INFO @ Sun, 21 Jun 2020 21:25:59: 13000000 INFO @ Sun, 21 Jun 2020 21:26:04: 9000000 INFO @ Sun, 21 Jun 2020 21:26:06: 14000000 INFO @ Sun, 21 Jun 2020 21:26:11: 10000000 INFO @ Sun, 21 Jun 2020 21:26:13: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:26:17: 11000000 INFO @ Sun, 21 Jun 2020 21:26:19: 16000000 INFO @ Sun, 21 Jun 2020 21:26:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:26:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:26:20: #1 total tags in treatment: 16054721 INFO @ Sun, 21 Jun 2020 21:26:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:20: #1 tags after filtering in treatment: 16054673 INFO @ Sun, 21 Jun 2020 21:26:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:20: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:22: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:26:22: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:26:24: 12000000 INFO @ Sun, 21 Jun 2020 21:26:30: 13000000 INFO @ Sun, 21 Jun 2020 21:26:37: 14000000 INFO @ Sun, 21 Jun 2020 21:26:44: 15000000 INFO @ Sun, 21 Jun 2020 21:26:50: 16000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:26:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:26:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:26:51: #1 total tags in treatment: 16054721 INFO @ Sun, 21 Jun 2020 21:26:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:51: #1 tags after filtering in treatment: 16054673 INFO @ Sun, 21 Jun 2020 21:26:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:52: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:26:52: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495261/SRX495261.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling