Job ID = 6458142 SRX = SRX495259 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:36:11 prefetch.2.10.7: 1) Downloading 'SRR1198764'... 2020-06-21T12:36:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:43:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:43:04 prefetch.2.10.7: 1) 'SRR1198764' was downloaded successfully Read 32193540 spots for SRR1198764/SRR1198764.sra Written 32193540 spots for SRR1198764/SRR1198764.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:11 32193540 reads; of these: 32193540 (100.00%) were unpaired; of these: 1596138 (4.96%) aligned 0 times 27524635 (85.50%) aligned exactly 1 time 3072767 (9.54%) aligned >1 times 95.04% overall alignment rate Time searching: 00:07:11 Overall time: 00:07:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7938110 / 30597402 = 0.2594 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:56: 1000000 INFO @ Sun, 21 Jun 2020 21:59:01: 2000000 INFO @ Sun, 21 Jun 2020 21:59:06: 3000000 INFO @ Sun, 21 Jun 2020 21:59:11: 4000000 INFO @ Sun, 21 Jun 2020 21:59:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:59:20: 6000000 INFO @ Sun, 21 Jun 2020 21:59:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:59:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:59:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:59:26: 7000000 INFO @ Sun, 21 Jun 2020 21:59:27: 1000000 INFO @ Sun, 21 Jun 2020 21:59:31: 8000000 INFO @ Sun, 21 Jun 2020 21:59:32: 2000000 INFO @ Sun, 21 Jun 2020 21:59:36: 9000000 INFO @ Sun, 21 Jun 2020 21:59:37: 3000000 INFO @ Sun, 21 Jun 2020 21:59:41: 10000000 INFO @ Sun, 21 Jun 2020 21:59:42: 4000000 INFO @ Sun, 21 Jun 2020 21:59:46: 11000000 INFO @ Sun, 21 Jun 2020 21:59:47: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:59:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:59:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:59:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:59:51: 12000000 INFO @ Sun, 21 Jun 2020 21:59:53: 6000000 INFO @ Sun, 21 Jun 2020 21:59:57: 13000000 INFO @ Sun, 21 Jun 2020 21:59:57: 1000000 INFO @ Sun, 21 Jun 2020 21:59:58: 7000000 INFO @ Sun, 21 Jun 2020 22:00:02: 14000000 INFO @ Sun, 21 Jun 2020 22:00:03: 8000000 INFO @ Sun, 21 Jun 2020 22:00:04: 2000000 INFO @ Sun, 21 Jun 2020 22:00:08: 15000000 INFO @ Sun, 21 Jun 2020 22:00:09: 9000000 INFO @ Sun, 21 Jun 2020 22:00:10: 3000000 INFO @ Sun, 21 Jun 2020 22:00:13: 16000000 INFO @ Sun, 21 Jun 2020 22:00:14: 10000000 INFO @ Sun, 21 Jun 2020 22:00:16: 4000000 INFO @ Sun, 21 Jun 2020 22:00:18: 17000000 INFO @ Sun, 21 Jun 2020 22:00:19: 11000000 INFO @ Sun, 21 Jun 2020 22:00:22: 5000000 INFO @ Sun, 21 Jun 2020 22:00:24: 18000000 INFO @ Sun, 21 Jun 2020 22:00:25: 12000000 INFO @ Sun, 21 Jun 2020 22:00:28: 6000000 INFO @ Sun, 21 Jun 2020 22:00:29: 19000000 INFO @ Sun, 21 Jun 2020 22:00:31: 13000000 INFO @ Sun, 21 Jun 2020 22:00:34: 7000000 INFO @ Sun, 21 Jun 2020 22:00:35: 20000000 INFO @ Sun, 21 Jun 2020 22:00:36: 14000000 INFO @ Sun, 21 Jun 2020 22:00:40: 21000000 INFO @ Sun, 21 Jun 2020 22:00:41: 8000000 INFO @ Sun, 21 Jun 2020 22:00:41: 15000000 INFO @ Sun, 21 Jun 2020 22:00:45: 22000000 INFO @ Sun, 21 Jun 2020 22:00:47: 9000000 INFO @ Sun, 21 Jun 2020 22:00:47: 16000000 INFO @ Sun, 21 Jun 2020 22:00:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:00:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:00:49: #1 total tags in treatment: 22659292 INFO @ Sun, 21 Jun 2020 22:00:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:00:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:00:50: #1 tags after filtering in treatment: 22659283 INFO @ Sun, 21 Jun 2020 22:00:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:00:50: #1 finished! INFO @ Sun, 21 Jun 2020 22:00:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:00:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:00:51: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:00:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:00:51: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 22:00:52: 17000000 INFO @ Sun, 21 Jun 2020 22:00:53: 10000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:00:58: 18000000 INFO @ Sun, 21 Jun 2020 22:00:59: 11000000 INFO @ Sun, 21 Jun 2020 22:01:03: 19000000 INFO @ Sun, 21 Jun 2020 22:01:05: 12000000 INFO @ Sun, 21 Jun 2020 22:01:09: 20000000 INFO @ Sun, 21 Jun 2020 22:01:11: 13000000 INFO @ Sun, 21 Jun 2020 22:01:14: 21000000 INFO @ Sun, 21 Jun 2020 22:01:17: 14000000 INFO @ Sun, 21 Jun 2020 22:01:20: 22000000 INFO @ Sun, 21 Jun 2020 22:01:24: 15000000 INFO @ Sun, 21 Jun 2020 22:01:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:01:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:01:24: #1 total tags in treatment: 22659292 INFO @ Sun, 21 Jun 2020 22:01:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:01:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:01:24: #1 tags after filtering in treatment: 22659283 INFO @ Sun, 21 Jun 2020 22:01:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:01:24: #1 finished! INFO @ Sun, 21 Jun 2020 22:01:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:01:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:01:26: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:01:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:01:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:01:29: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:01:35: 17000000 INFO @ Sun, 21 Jun 2020 22:01:41: 18000000 INFO @ Sun, 21 Jun 2020 22:01:48: 19000000 INFO @ Sun, 21 Jun 2020 22:01:53: 20000000 INFO @ Sun, 21 Jun 2020 22:01:59: 21000000 INFO @ Sun, 21 Jun 2020 22:02:05: 22000000 INFO @ Sun, 21 Jun 2020 22:02:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:02:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:02:09: #1 total tags in treatment: 22659292 INFO @ Sun, 21 Jun 2020 22:02:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:02:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:02:10: #1 tags after filtering in treatment: 22659283 INFO @ Sun, 21 Jun 2020 22:02:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:02:10: #1 finished! INFO @ Sun, 21 Jun 2020 22:02:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:02:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:02:11: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:02:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:02:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495259/SRX495259.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。