Job ID = 6458138 SRX = SRX495255 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:12:49 prefetch.2.10.7: 1) Downloading 'SRR1198760'... 2020-06-21T12:12:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:59 prefetch.2.10.7: 1) 'SRR1198760' was downloaded successfully Read 24517599 spots for SRR1198760/SRR1198760.sra Written 24517599 spots for SRR1198760/SRR1198760.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 24517599 reads; of these: 24517599 (100.00%) were unpaired; of these: 587600 (2.40%) aligned 0 times 19274429 (78.61%) aligned exactly 1 time 4655570 (18.99%) aligned >1 times 97.60% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5788081 / 23929999 = 0.2419 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:29:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:29:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:29:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:29:36: 1000000 INFO @ Sun, 21 Jun 2020 21:29:42: 2000000 INFO @ Sun, 21 Jun 2020 21:29:49: 3000000 INFO @ Sun, 21 Jun 2020 21:29:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:29:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:29:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:29:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:30:02: 5000000 INFO @ Sun, 21 Jun 2020 21:30:06: 1000000 INFO @ Sun, 21 Jun 2020 21:30:08: 6000000 INFO @ Sun, 21 Jun 2020 21:30:12: 2000000 INFO @ Sun, 21 Jun 2020 21:30:15: 7000000 INFO @ Sun, 21 Jun 2020 21:30:19: 3000000 INFO @ Sun, 21 Jun 2020 21:30:21: 8000000 INFO @ Sun, 21 Jun 2020 21:30:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:30:27: 9000000 INFO @ Sun, 21 Jun 2020 21:30:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:30:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:30:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:30:32: 5000000 INFO @ Sun, 21 Jun 2020 21:30:34: 10000000 INFO @ Sun, 21 Jun 2020 21:30:36: 1000000 INFO @ Sun, 21 Jun 2020 21:30:38: 6000000 INFO @ Sun, 21 Jun 2020 21:30:40: 11000000 INFO @ Sun, 21 Jun 2020 21:30:42: 2000000 INFO @ Sun, 21 Jun 2020 21:30:44: 7000000 INFO @ Sun, 21 Jun 2020 21:30:46: 12000000 INFO @ Sun, 21 Jun 2020 21:30:49: 3000000 INFO @ Sun, 21 Jun 2020 21:30:51: 8000000 INFO @ Sun, 21 Jun 2020 21:30:53: 13000000 INFO @ Sun, 21 Jun 2020 21:30:55: 4000000 INFO @ Sun, 21 Jun 2020 21:30:57: 9000000 INFO @ Sun, 21 Jun 2020 21:30:59: 14000000 INFO @ Sun, 21 Jun 2020 21:31:02: 5000000 INFO @ Sun, 21 Jun 2020 21:31:03: 10000000 INFO @ Sun, 21 Jun 2020 21:31:06: 15000000 INFO @ Sun, 21 Jun 2020 21:31:08: 6000000 INFO @ Sun, 21 Jun 2020 21:31:09: 11000000 INFO @ Sun, 21 Jun 2020 21:31:12: 16000000 INFO @ Sun, 21 Jun 2020 21:31:14: 7000000 INFO @ Sun, 21 Jun 2020 21:31:15: 12000000 INFO @ Sun, 21 Jun 2020 21:31:18: 17000000 INFO @ Sun, 21 Jun 2020 21:31:20: 8000000 INFO @ Sun, 21 Jun 2020 21:31:21: 13000000 INFO @ Sun, 21 Jun 2020 21:31:25: 18000000 INFO @ Sun, 21 Jun 2020 21:31:26: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:31:26: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:31:26: #1 total tags in treatment: 18141918 INFO @ Sun, 21 Jun 2020 21:31:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:31:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:31:26: 9000000 INFO @ Sun, 21 Jun 2020 21:31:27: #1 tags after filtering in treatment: 18141916 INFO @ Sun, 21 Jun 2020 21:31:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:31:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:31:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:31:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:31:27: 14000000 INFO @ Sun, 21 Jun 2020 21:31:28: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:31:28: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:31:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:31:32: 10000000 INFO @ Sun, 21 Jun 2020 21:31:33: 15000000 INFO @ Sun, 21 Jun 2020 21:31:37: 11000000 INFO @ Sun, 21 Jun 2020 21:31:39: 16000000 INFO @ Sun, 21 Jun 2020 21:31:43: 12000000 INFO @ Sun, 21 Jun 2020 21:31:44: 17000000 INFO @ Sun, 21 Jun 2020 21:31:48: 13000000 INFO @ Sun, 21 Jun 2020 21:31:50: 18000000 INFO @ Sun, 21 Jun 2020 21:31:51: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:31:51: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:31:51: #1 total tags in treatment: 18141918 INFO @ Sun, 21 Jun 2020 21:31:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:31:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:31:51: #1 tags after filtering in treatment: 18141916 INFO @ Sun, 21 Jun 2020 21:31:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:31:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:31:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:31:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:31:53: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:31:53: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:31:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:31:53: 14000000 INFO @ Sun, 21 Jun 2020 21:31:58: 15000000 INFO @ Sun, 21 Jun 2020 21:32:03: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:32:08: 17000000 INFO @ Sun, 21 Jun 2020 21:32:14: 18000000 INFO @ Sun, 21 Jun 2020 21:32:15: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:32:15: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:32:15: #1 total tags in treatment: 18141918 INFO @ Sun, 21 Jun 2020 21:32:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:32:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:32:15: #1 tags after filtering in treatment: 18141916 INFO @ Sun, 21 Jun 2020 21:32:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:32:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:32:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:32:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:32:17: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:32:17: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:32:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495255/SRX495255.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。