Job ID = 6458137 SRX = SRX495254 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:18:34 prefetch.2.10.7: 1) Downloading 'SRR1198759'... 2020-06-21T12:18:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:19 prefetch.2.10.7: 1) 'SRR1198759' was downloaded successfully Read 20889019 spots for SRR1198759/SRR1198759.sra Written 20889019 spots for SRR1198759/SRR1198759.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:04 20889019 reads; of these: 20889019 (100.00%) were unpaired; of these: 2966305 (14.20%) aligned 0 times 14861853 (71.15%) aligned exactly 1 time 3060861 (14.65%) aligned >1 times 85.80% overall alignment rate Time searching: 00:05:04 Overall time: 00:05:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4120692 / 17922714 = 0.2299 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:13: 1000000 INFO @ Sun, 21 Jun 2020 21:32:20: 2000000 INFO @ Sun, 21 Jun 2020 21:32:27: 3000000 INFO @ Sun, 21 Jun 2020 21:32:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:37: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:37: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:40: 5000000 INFO @ Sun, 21 Jun 2020 21:32:43: 1000000 INFO @ Sun, 21 Jun 2020 21:32:47: 6000000 INFO @ Sun, 21 Jun 2020 21:32:49: 2000000 INFO @ Sun, 21 Jun 2020 21:32:54: 7000000 INFO @ Sun, 21 Jun 2020 21:32:56: 3000000 INFO @ Sun, 21 Jun 2020 21:33:01: 8000000 INFO @ Sun, 21 Jun 2020 21:33:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:08: 5000000 INFO @ Sun, 21 Jun 2020 21:33:08: 9000000 INFO @ Sun, 21 Jun 2020 21:33:14: 1000000 INFO @ Sun, 21 Jun 2020 21:33:14: 6000000 INFO @ Sun, 21 Jun 2020 21:33:15: 10000000 INFO @ Sun, 21 Jun 2020 21:33:20: 7000000 INFO @ Sun, 21 Jun 2020 21:33:21: 2000000 INFO @ Sun, 21 Jun 2020 21:33:23: 11000000 INFO @ Sun, 21 Jun 2020 21:33:26: 8000000 INFO @ Sun, 21 Jun 2020 21:33:28: 3000000 INFO @ Sun, 21 Jun 2020 21:33:30: 12000000 INFO @ Sun, 21 Jun 2020 21:33:33: 9000000 INFO @ Sun, 21 Jun 2020 21:33:35: 4000000 INFO @ Sun, 21 Jun 2020 21:33:37: 13000000 INFO @ Sun, 21 Jun 2020 21:33:39: 10000000 INFO @ Sun, 21 Jun 2020 21:33:43: 5000000 INFO @ Sun, 21 Jun 2020 21:33:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:33:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:33:44: #1 total tags in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:33:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:33:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:33:44: #1 tags after filtering in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:33:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:33:44: #1 finished! INFO @ Sun, 21 Jun 2020 21:33:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:33:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:33:45: 11000000 INFO @ Sun, 21 Jun 2020 21:33:45: #2 number of paired peaks: 43 WARNING @ Sun, 21 Jun 2020 21:33:45: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:33:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:33:50: 6000000 INFO @ Sun, 21 Jun 2020 21:33:52: 12000000 INFO @ Sun, 21 Jun 2020 21:33:57: 7000000 INFO @ Sun, 21 Jun 2020 21:33:58: 13000000 INFO @ Sun, 21 Jun 2020 21:34:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:34:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:34:03: #1 total tags in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:34:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:34:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:34:03: #1 tags after filtering in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:34:03: #1 finished! INFO @ Sun, 21 Jun 2020 21:34:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:34:04: 8000000 INFO @ Sun, 21 Jun 2020 21:34:04: #2 number of paired peaks: 43 WARNING @ Sun, 21 Jun 2020 21:34:04: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:34:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:34:10: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:34:17: 10000000 INFO @ Sun, 21 Jun 2020 21:34:23: 11000000 INFO @ Sun, 21 Jun 2020 21:34:30: 12000000 INFO @ Sun, 21 Jun 2020 21:34:37: 13000000 INFO @ Sun, 21 Jun 2020 21:34:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:34:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:34:43: #1 total tags in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:34:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:34:43: #1 tags after filtering in treatment: 13802022 INFO @ Sun, 21 Jun 2020 21:34:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:34:43: #1 finished! INFO @ Sun, 21 Jun 2020 21:34:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:34:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:34:44: #2 number of paired peaks: 43 WARNING @ Sun, 21 Jun 2020 21:34:44: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:34:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495254/SRX495254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。