Job ID = 6508878 SRX = SRX495251 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:03:40 prefetch.2.10.7: 1) Downloading 'SRR1198756'... 2020-06-26T15:03:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:06:09 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:06:09 prefetch.2.10.7: 1) 'SRR1198756' was downloaded successfully Read 19875673 spots for SRR1198756/SRR1198756.sra Written 19875673 spots for SRR1198756/SRR1198756.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:54 19875673 reads; of these: 19875673 (100.00%) were unpaired; of these: 861837 (4.34%) aligned 0 times 16274786 (81.88%) aligned exactly 1 time 2739050 (13.78%) aligned >1 times 95.66% overall alignment rate Time searching: 00:05:54 Overall time: 00:05:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3601277 / 19013836 = 0.1894 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:18:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:18:44: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:18:44: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:18:51: 1000000 INFO @ Sat, 27 Jun 2020 00:18:58: 2000000 INFO @ Sat, 27 Jun 2020 00:19:05: 3000000 INFO @ Sat, 27 Jun 2020 00:19:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:19:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:19:14: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:19:14: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:19:18: 5000000 INFO @ Sat, 27 Jun 2020 00:19:21: 1000000 INFO @ Sat, 27 Jun 2020 00:19:24: 6000000 INFO @ Sat, 27 Jun 2020 00:19:28: 2000000 INFO @ Sat, 27 Jun 2020 00:19:31: 7000000 INFO @ Sat, 27 Jun 2020 00:19:35: 3000000 INFO @ Sat, 27 Jun 2020 00:19:37: 8000000 BedGraph に変換中... INFO @ Sat, 27 Jun 2020 00:19:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:19:43: 9000000 INFO @ Sat, 27 Jun 2020 00:19:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:19:46: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:19:46: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:19:50: 5000000 INFO @ Sat, 27 Jun 2020 00:19:50: 10000000 INFO @ Sat, 27 Jun 2020 00:19:53: 1000000 INFO @ Sat, 27 Jun 2020 00:19:57: 6000000 INFO @ Sat, 27 Jun 2020 00:19:58: 11000000 INFO @ Sat, 27 Jun 2020 00:20:01: 2000000 INFO @ Sat, 27 Jun 2020 00:20:04: 7000000 INFO @ Sat, 27 Jun 2020 00:20:05: 12000000 INFO @ Sat, 27 Jun 2020 00:20:09: 3000000 INFO @ Sat, 27 Jun 2020 00:20:11: 8000000 INFO @ Sat, 27 Jun 2020 00:20:12: 13000000 INFO @ Sat, 27 Jun 2020 00:20:16: 4000000 INFO @ Sat, 27 Jun 2020 00:20:18: 14000000 INFO @ Sat, 27 Jun 2020 00:20:19: 9000000 INFO @ Sat, 27 Jun 2020 00:20:23: 5000000 INFO @ Sat, 27 Jun 2020 00:20:24: 15000000 INFO @ Sat, 27 Jun 2020 00:20:26: 10000000 INFO @ Sat, 27 Jun 2020 00:20:27: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:20:27: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:20:27: #1 total tags in treatment: 15412559 INFO @ Sat, 27 Jun 2020 00:20:27: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:20:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:20:28: #1 tags after filtering in treatment: 15412533 INFO @ Sat, 27 Jun 2020 00:20:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:20:28: #1 finished! INFO @ Sat, 27 Jun 2020 00:20:28: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:20:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:20:29: #2 number of paired peaks: 34 WARNING @ Sat, 27 Jun 2020 00:20:29: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:20:29: Process for pairing-model is terminated! INFO @ Sat, 27 Jun 2020 00:20:29: 6000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 13 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:20:33: 11000000 INFO @ Sat, 27 Jun 2020 00:20:36: 7000000 INFO @ Sat, 27 Jun 2020 00:20:40: 12000000 INFO @ Sat, 27 Jun 2020 00:20:42: 8000000 INFO @ Sat, 27 Jun 2020 00:20:48: 13000000 INFO @ Sat, 27 Jun 2020 00:20:49: 9000000 INFO @ Sat, 27 Jun 2020 00:20:55: 14000000 INFO @ Sat, 27 Jun 2020 00:20:56: 10000000 INFO @ Sat, 27 Jun 2020 00:21:03: 15000000 INFO @ Sat, 27 Jun 2020 00:21:03: 11000000 INFO @ Sat, 27 Jun 2020 00:21:06: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:21:06: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:21:06: #1 total tags in treatment: 15412559 INFO @ Sat, 27 Jun 2020 00:21:06: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:21:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:21:06: #1 tags after filtering in treatment: 15412533 INFO @ Sat, 27 Jun 2020 00:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:21:06: #1 finished! INFO @ Sat, 27 Jun 2020 00:21:06: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:21:08: #2 number of paired peaks: 34 WARNING @ Sat, 27 Jun 2020 00:21:08: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:21:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:21:09: 12000000 INFO @ Sat, 27 Jun 2020 00:21:16: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:21:22: 14000000 INFO @ Sat, 27 Jun 2020 00:21:28: 15000000 INFO @ Sat, 27 Jun 2020 00:21:31: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:21:31: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:21:31: #1 total tags in treatment: 15412559 INFO @ Sat, 27 Jun 2020 00:21:31: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:21:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:21:31: #1 tags after filtering in treatment: 15412533 INFO @ Sat, 27 Jun 2020 00:21:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:21:31: #1 finished! INFO @ Sat, 27 Jun 2020 00:21:31: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:21:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:21:32: #2 number of paired peaks: 34 WARNING @ Sat, 27 Jun 2020 00:21:32: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:21:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495251/SRX495251.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。