Job ID = 6508877 SRX = SRX495250 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:17:14 prefetch.2.10.7: 1) Downloading 'SRR1198755'... 2020-06-26T15:17:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:19:38 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:19:38 prefetch.2.10.7: 1) 'SRR1198755' was downloaded successfully Read 24331776 spots for SRR1198755/SRR1198755.sra Written 24331776 spots for SRR1198755/SRR1198755.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:45 24331776 reads; of these: 24331776 (100.00%) were unpaired; of these: 6464903 (26.57%) aligned 0 times 14765222 (60.68%) aligned exactly 1 time 3101651 (12.75%) aligned >1 times 73.43% overall alignment rate Time searching: 00:04:45 Overall time: 00:04:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4103161 / 17866873 = 0.2297 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:30:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:04: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:04: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:10: 1000000 INFO @ Sat, 27 Jun 2020 00:30:16: 2000000 INFO @ Sat, 27 Jun 2020 00:30:22: 3000000 INFO @ Sat, 27 Jun 2020 00:30:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:30:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:34: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:34: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:34: 5000000 INFO @ Sat, 27 Jun 2020 00:30:41: 1000000 INFO @ Sat, 27 Jun 2020 00:30:41: 6000000 INFO @ Sat, 27 Jun 2020 00:30:48: 2000000 INFO @ Sat, 27 Jun 2020 00:30:49: 7000000 INFO @ Sat, 27 Jun 2020 00:30:56: 3000000 INFO @ Sat, 27 Jun 2020 00:30:56: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:31:03: 4000000 INFO @ Sat, 27 Jun 2020 00:31:04: 9000000 INFO @ Sat, 27 Jun 2020 00:31:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:31:05: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:31:05: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:31:11: 5000000 INFO @ Sat, 27 Jun 2020 00:31:13: 10000000 INFO @ Sat, 27 Jun 2020 00:31:13: 1000000 INFO @ Sat, 27 Jun 2020 00:31:18: 6000000 INFO @ Sat, 27 Jun 2020 00:31:21: 2000000 INFO @ Sat, 27 Jun 2020 00:31:21: 11000000 INFO @ Sat, 27 Jun 2020 00:31:26: 7000000 INFO @ Sat, 27 Jun 2020 00:31:29: 3000000 INFO @ Sat, 27 Jun 2020 00:31:30: 12000000 INFO @ Sat, 27 Jun 2020 00:31:34: 8000000 INFO @ Sat, 27 Jun 2020 00:31:37: 4000000 INFO @ Sat, 27 Jun 2020 00:31:38: 13000000 INFO @ Sat, 27 Jun 2020 00:31:42: 9000000 INFO @ Sat, 27 Jun 2020 00:31:45: 5000000 INFO @ Sat, 27 Jun 2020 00:31:45: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:31:45: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:31:45: #1 total tags in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:31:45: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:31:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:31:46: #1 tags after filtering in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:31:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:31:46: #1 finished! INFO @ Sat, 27 Jun 2020 00:31:46: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:31:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:31:47: #2 number of paired peaks: 71 WARNING @ Sat, 27 Jun 2020 00:31:47: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:31:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:31:49: 10000000 INFO @ Sat, 27 Jun 2020 00:31:53: 6000000 INFO @ Sat, 27 Jun 2020 00:31:57: 11000000 INFO @ Sat, 27 Jun 2020 00:32:00: 7000000 INFO @ Sat, 27 Jun 2020 00:32:04: 12000000 INFO @ Sat, 27 Jun 2020 00:32:07: 8000000 INFO @ Sat, 27 Jun 2020 00:32:11: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:32:15: 9000000 INFO @ Sat, 27 Jun 2020 00:32:17: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:32:17: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:32:17: #1 total tags in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:32:17: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:32:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:32:18: #1 tags after filtering in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:32:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:32:18: #1 finished! INFO @ Sat, 27 Jun 2020 00:32:18: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:32:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:32:19: #2 number of paired peaks: 71 WARNING @ Sat, 27 Jun 2020 00:32:19: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:32:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:32:22: 10000000 INFO @ Sat, 27 Jun 2020 00:32:28: 11000000 INFO @ Sat, 27 Jun 2020 00:32:36: 12000000 INFO @ Sat, 27 Jun 2020 00:32:43: 13000000 INFO @ Sat, 27 Jun 2020 00:32:49: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:32:49: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:32:49: #1 total tags in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:32:49: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:32:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:32:49: #1 tags after filtering in treatment: 13763712 INFO @ Sat, 27 Jun 2020 00:32:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:32:49: #1 finished! INFO @ Sat, 27 Jun 2020 00:32:49: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:32:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:32:50: #2 number of paired peaks: 71 WARNING @ Sat, 27 Jun 2020 00:32:50: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:32:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495250/SRX495250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling