Job ID = 6508873 SRX = SRX495246 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:06:29 prefetch.2.10.7: 1) Downloading 'SRR1198751'... 2020-06-26T15:06:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:10:28 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:10:28 prefetch.2.10.7: 1) 'SRR1198751' was downloaded successfully Read 30861048 spots for SRR1198751/SRR1198751.sra Written 30861048 spots for SRR1198751/SRR1198751.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:48 30861048 reads; of these: 30861048 (100.00%) were unpaired; of these: 3668349 (11.89%) aligned 0 times 22467997 (72.80%) aligned exactly 1 time 4724702 (15.31%) aligned >1 times 88.11% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6007298 / 27192699 = 0.2209 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:26:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:26:49: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:26:49: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:26:56: 1000000 INFO @ Sat, 27 Jun 2020 00:27:02: 2000000 INFO @ Sat, 27 Jun 2020 00:27:08: 3000000 INFO @ Sat, 27 Jun 2020 00:27:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:27:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:27:19: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:27:19: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:27:21: 5000000 INFO @ Sat, 27 Jun 2020 00:27:26: 1000000 INFO @ Sat, 27 Jun 2020 00:27:27: 6000000 INFO @ Sat, 27 Jun 2020 00:27:33: 2000000 INFO @ Sat, 27 Jun 2020 00:27:34: 7000000 INFO @ Sat, 27 Jun 2020 00:27:40: 3000000 INFO @ Sat, 27 Jun 2020 00:27:41: 8000000 INFO @ Sat, 27 Jun 2020 00:27:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:27:48: 9000000 INFO @ Sat, 27 Jun 2020 00:27:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:27:49: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:27:49: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:27:54: 5000000 INFO @ Sat, 27 Jun 2020 00:27:56: 10000000 INFO @ Sat, 27 Jun 2020 00:27:58: 1000000 INFO @ Sat, 27 Jun 2020 00:28:02: 6000000 INFO @ Sat, 27 Jun 2020 00:28:03: 11000000 INFO @ Sat, 27 Jun 2020 00:28:06: 2000000 INFO @ Sat, 27 Jun 2020 00:28:09: 7000000 INFO @ Sat, 27 Jun 2020 00:28:10: 12000000 INFO @ Sat, 27 Jun 2020 00:28:15: 3000000 INFO @ Sat, 27 Jun 2020 00:28:16: 8000000 INFO @ Sat, 27 Jun 2020 00:28:18: 13000000 INFO @ Sat, 27 Jun 2020 00:28:23: 4000000 INFO @ Sat, 27 Jun 2020 00:28:24: 9000000 INFO @ Sat, 27 Jun 2020 00:28:25: 14000000 INFO @ Sat, 27 Jun 2020 00:28:31: 5000000 INFO @ Sat, 27 Jun 2020 00:28:31: 10000000 INFO @ Sat, 27 Jun 2020 00:28:33: 15000000 INFO @ Sat, 27 Jun 2020 00:28:39: 6000000 INFO @ Sat, 27 Jun 2020 00:28:39: 11000000 INFO @ Sat, 27 Jun 2020 00:28:40: 16000000 INFO @ Sat, 27 Jun 2020 00:28:46: 12000000 INFO @ Sat, 27 Jun 2020 00:28:47: 7000000 INFO @ Sat, 27 Jun 2020 00:28:48: 17000000 INFO @ Sat, 27 Jun 2020 00:28:54: 13000000 INFO @ Sat, 27 Jun 2020 00:28:55: 8000000 INFO @ Sat, 27 Jun 2020 00:28:56: 18000000 INFO @ Sat, 27 Jun 2020 00:29:01: 14000000 INFO @ Sat, 27 Jun 2020 00:29:03: 9000000 INFO @ Sat, 27 Jun 2020 00:29:04: 19000000 INFO @ Sat, 27 Jun 2020 00:29:09: 15000000 INFO @ Sat, 27 Jun 2020 00:29:11: 10000000 INFO @ Sat, 27 Jun 2020 00:29:11: 20000000 INFO @ Sat, 27 Jun 2020 00:29:17: 16000000 INFO @ Sat, 27 Jun 2020 00:29:19: 11000000 INFO @ Sat, 27 Jun 2020 00:29:20: 21000000 INFO @ Sat, 27 Jun 2020 00:29:21: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:29:21: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:29:21: #1 total tags in treatment: 21185401 INFO @ Sat, 27 Jun 2020 00:29:21: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:29:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:29:22: #1 tags after filtering in treatment: 21185399 INFO @ Sat, 27 Jun 2020 00:29:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:29:22: #1 finished! INFO @ Sat, 27 Jun 2020 00:29:22: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:29:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:29:23: #2 number of paired peaks: 25 WARNING @ Sat, 27 Jun 2020 00:29:23: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:29:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:29:24: 17000000 INFO @ Sat, 27 Jun 2020 00:29:27: 12000000 INFO @ Sat, 27 Jun 2020 00:29:32: 18000000 INFO @ Sat, 27 Jun 2020 00:29:35: 13000000 INFO @ Sat, 27 Jun 2020 00:29:40: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:29:43: 14000000 INFO @ Sat, 27 Jun 2020 00:29:47: 20000000 INFO @ Sat, 27 Jun 2020 00:29:52: 15000000 INFO @ Sat, 27 Jun 2020 00:29:55: 21000000 INFO @ Sat, 27 Jun 2020 00:29:56: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:29:56: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:29:56: #1 total tags in treatment: 21185401 INFO @ Sat, 27 Jun 2020 00:29:56: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:29:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:29:57: #1 tags after filtering in treatment: 21185399 INFO @ Sat, 27 Jun 2020 00:29:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:29:57: #1 finished! INFO @ Sat, 27 Jun 2020 00:29:57: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:29:59: #2 number of paired peaks: 25 WARNING @ Sat, 27 Jun 2020 00:29:59: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:29:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:30:00: 16000000 INFO @ Sat, 27 Jun 2020 00:30:07: 17000000 INFO @ Sat, 27 Jun 2020 00:30:16: 18000000 INFO @ Sat, 27 Jun 2020 00:30:23: 19000000 INFO @ Sat, 27 Jun 2020 00:30:31: 20000000 INFO @ Sat, 27 Jun 2020 00:30:39: 21000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:30:40: #1 tag size is determined as 50 bps INFO @ Sat, 27 Jun 2020 00:30:40: #1 tag size = 50 INFO @ Sat, 27 Jun 2020 00:30:40: #1 total tags in treatment: 21185401 INFO @ Sat, 27 Jun 2020 00:30:40: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:30:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:30:41: #1 tags after filtering in treatment: 21185399 INFO @ Sat, 27 Jun 2020 00:30:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:30:41: #1 finished! INFO @ Sat, 27 Jun 2020 00:30:41: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:30:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:30:42: #2 number of paired peaks: 25 WARNING @ Sat, 27 Jun 2020 00:30:42: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:30:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495246/SRX495246.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling