Job ID = 6508868 SRX = SRX495241 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:52:22 prefetch.2.10.7: 1) Downloading 'SRR1198746'... 2020-06-26T14:52:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:54:02 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:54:02 prefetch.2.10.7: 1) 'SRR1198746' was downloaded successfully Read 22749432 spots for SRR1198746/SRR1198746.sra Written 22749432 spots for SRR1198746/SRR1198746.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:55 22749432 reads; of these: 22749432 (100.00%) were unpaired; of these: 1041729 (4.58%) aligned 0 times 17936896 (78.85%) aligned exactly 1 time 3770807 (16.58%) aligned >1 times 95.42% overall alignment rate Time searching: 00:04:55 Overall time: 00:04:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4104595 / 21707703 = 0.1891 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:05:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:05:07: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:05:07: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:05:13: 1000000 INFO @ Sat, 27 Jun 2020 00:05:19: 2000000 INFO @ Sat, 27 Jun 2020 00:05:25: 3000000 INFO @ Sat, 27 Jun 2020 00:05:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:05:36: 5000000 INFO @ Sat, 27 Jun 2020 00:05:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:05:37: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:05:37: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:05:42: 6000000 INFO @ Sat, 27 Jun 2020 00:05:44: 1000000 INFO @ Sat, 27 Jun 2020 00:05:49: 7000000 INFO @ Sat, 27 Jun 2020 00:05:50: 2000000 INFO @ Sat, 27 Jun 2020 00:05:55: 8000000 INFO @ Sat, 27 Jun 2020 00:05:57: 3000000 INFO @ Sat, 27 Jun 2020 00:06:02: 9000000 INFO @ Sat, 27 Jun 2020 00:06:03: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:06:07: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:06:07: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:06:08: 10000000 INFO @ Sat, 27 Jun 2020 00:06:10: 5000000 INFO @ Sat, 27 Jun 2020 00:06:15: 1000000 INFO @ Sat, 27 Jun 2020 00:06:16: 11000000 INFO @ Sat, 27 Jun 2020 00:06:18: 6000000 INFO @ Sat, 27 Jun 2020 00:06:23: 2000000 INFO @ Sat, 27 Jun 2020 00:06:23: 12000000 INFO @ Sat, 27 Jun 2020 00:06:25: 7000000 INFO @ Sat, 27 Jun 2020 00:06:30: 13000000 INFO @ Sat, 27 Jun 2020 00:06:31: 3000000 INFO @ Sat, 27 Jun 2020 00:06:32: 8000000 INFO @ Sat, 27 Jun 2020 00:06:38: 14000000 INFO @ Sat, 27 Jun 2020 00:06:38: 4000000 INFO @ Sat, 27 Jun 2020 00:06:40: 9000000 INFO @ Sat, 27 Jun 2020 00:06:46: 15000000 INFO @ Sat, 27 Jun 2020 00:06:46: 5000000 INFO @ Sat, 27 Jun 2020 00:06:47: 10000000 INFO @ Sat, 27 Jun 2020 00:06:53: 16000000 INFO @ Sat, 27 Jun 2020 00:06:54: 6000000 INFO @ Sat, 27 Jun 2020 00:06:55: 11000000 INFO @ Sat, 27 Jun 2020 00:07:00: 17000000 INFO @ Sat, 27 Jun 2020 00:07:01: 7000000 INFO @ Sat, 27 Jun 2020 00:07:02: 12000000 INFO @ Sat, 27 Jun 2020 00:07:05: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:07:05: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:07:05: #1 total tags in treatment: 17603108 INFO @ Sat, 27 Jun 2020 00:07:05: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:07:06: #1 tags after filtering in treatment: 17603100 INFO @ Sat, 27 Jun 2020 00:07:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:07:06: #1 finished! INFO @ Sat, 27 Jun 2020 00:07:06: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:07:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:07:07: #2 number of paired peaks: 4 WARNING @ Sat, 27 Jun 2020 00:07:07: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:07:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:07:09: 8000000 INFO @ Sat, 27 Jun 2020 00:07:09: 13000000 INFO @ Sat, 27 Jun 2020 00:07:17: 14000000 INFO @ Sat, 27 Jun 2020 00:07:17: 9000000 INFO @ Sat, 27 Jun 2020 00:07:24: 15000000 INFO @ Sat, 27 Jun 2020 00:07:24: 10000000 INFO @ Sat, 27 Jun 2020 00:07:32: 16000000 INFO @ Sat, 27 Jun 2020 00:07:32: 11000000 INFO @ Sat, 27 Jun 2020 00:07:39: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:07:40: 12000000 INFO @ Sat, 27 Jun 2020 00:07:44: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:07:44: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:07:44: #1 total tags in treatment: 17603108 INFO @ Sat, 27 Jun 2020 00:07:44: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:07:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:07:44: #1 tags after filtering in treatment: 17603100 INFO @ Sat, 27 Jun 2020 00:07:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:07:44: #1 finished! INFO @ Sat, 27 Jun 2020 00:07:44: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:07:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:07:45: #2 number of paired peaks: 4 WARNING @ Sat, 27 Jun 2020 00:07:45: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:07:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:07:47: 13000000 INFO @ Sat, 27 Jun 2020 00:07:54: 14000000 INFO @ Sat, 27 Jun 2020 00:08:01: 15000000 INFO @ Sat, 27 Jun 2020 00:08:08: 16000000 INFO @ Sat, 27 Jun 2020 00:08:15: 17000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:08:20: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:08:20: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:08:20: #1 total tags in treatment: 17603108 INFO @ Sat, 27 Jun 2020 00:08:20: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:08:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:08:20: #1 tags after filtering in treatment: 17603100 INFO @ Sat, 27 Jun 2020 00:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:08:20: #1 finished! INFO @ Sat, 27 Jun 2020 00:08:20: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:08:22: #2 number of paired peaks: 4 WARNING @ Sat, 27 Jun 2020 00:08:22: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:08:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495241/SRX495241.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling