Job ID = 6508867 SRX = SRX495240 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:57:59 prefetch.2.10.7: 1) Downloading 'SRR1198745'... 2020-06-26T14:57:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:59:39 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:59:39 prefetch.2.10.7: 1) 'SRR1198745' was downloaded successfully Read 17486457 spots for SRR1198745/SRR1198745.sra Written 17486457 spots for SRR1198745/SRR1198745.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:05 17486457 reads; of these: 17486457 (100.00%) were unpaired; of these: 1183292 (6.77%) aligned 0 times 13963819 (79.86%) aligned exactly 1 time 2339346 (13.38%) aligned >1 times 93.23% overall alignment rate Time searching: 00:04:05 Overall time: 00:04:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3563817 / 16303165 = 0.2186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:08:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:08:50: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:08:50: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:08:56: 1000000 INFO @ Sat, 27 Jun 2020 00:09:02: 2000000 INFO @ Sat, 27 Jun 2020 00:09:09: 3000000 INFO @ Sat, 27 Jun 2020 00:09:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:09:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:09:20: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:09:20: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:09:21: 5000000 INFO @ Sat, 27 Jun 2020 00:09:27: 1000000 INFO @ Sat, 27 Jun 2020 00:09:28: 6000000 INFO @ Sat, 27 Jun 2020 00:09:34: 2000000 INFO @ Sat, 27 Jun 2020 00:09:35: 7000000 INFO @ Sat, 27 Jun 2020 00:09:41: 3000000 INFO @ Sat, 27 Jun 2020 00:09:41: 8000000 INFO @ Sat, 27 Jun 2020 00:09:47: 4000000 INFO @ Sat, 27 Jun 2020 00:09:48: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:09:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:09:50: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:09:50: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:09:54: 5000000 INFO @ Sat, 27 Jun 2020 00:09:55: 10000000 INFO @ Sat, 27 Jun 2020 00:09:57: 1000000 INFO @ Sat, 27 Jun 2020 00:10:01: 6000000 INFO @ Sat, 27 Jun 2020 00:10:02: 11000000 INFO @ Sat, 27 Jun 2020 00:10:04: 2000000 INFO @ Sat, 27 Jun 2020 00:10:08: 7000000 INFO @ Sat, 27 Jun 2020 00:10:09: 12000000 INFO @ Sat, 27 Jun 2020 00:10:11: 3000000 INFO @ Sat, 27 Jun 2020 00:10:14: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:10:14: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:10:14: #1 total tags in treatment: 12739348 INFO @ Sat, 27 Jun 2020 00:10:14: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:10:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:10:15: #1 tags after filtering in treatment: 12739330 INFO @ Sat, 27 Jun 2020 00:10:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:10:15: #1 finished! INFO @ Sat, 27 Jun 2020 00:10:15: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:10:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:10:15: 8000000 INFO @ Sat, 27 Jun 2020 00:10:16: #2 number of paired peaks: 70 WARNING @ Sat, 27 Jun 2020 00:10:16: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:10:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:10:18: 4000000 INFO @ Sat, 27 Jun 2020 00:10:22: 9000000 INFO @ Sat, 27 Jun 2020 00:10:25: 5000000 INFO @ Sat, 27 Jun 2020 00:10:29: 10000000 INFO @ Sat, 27 Jun 2020 00:10:32: 6000000 INFO @ Sat, 27 Jun 2020 00:10:36: 11000000 INFO @ Sat, 27 Jun 2020 00:10:39: 7000000 INFO @ Sat, 27 Jun 2020 00:10:43: 12000000 INFO @ Sat, 27 Jun 2020 00:10:46: 8000000 INFO @ Sat, 27 Jun 2020 00:10:49: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:10:49: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:10:49: #1 total tags in treatment: 12739348 INFO @ Sat, 27 Jun 2020 00:10:49: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:10:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:10:49: #1 tags after filtering in treatment: 12739330 INFO @ Sat, 27 Jun 2020 00:10:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:10:49: #1 finished! INFO @ Sat, 27 Jun 2020 00:10:49: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:10:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:10:50: #2 number of paired peaks: 70 WARNING @ Sat, 27 Jun 2020 00:10:50: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:10:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:10:52: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:10:59: 10000000 INFO @ Sat, 27 Jun 2020 00:11:05: 11000000 INFO @ Sat, 27 Jun 2020 00:11:12: 12000000 INFO @ Sat, 27 Jun 2020 00:11:17: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:11:17: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:11:17: #1 total tags in treatment: 12739348 INFO @ Sat, 27 Jun 2020 00:11:17: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:11:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:11:17: #1 tags after filtering in treatment: 12739330 INFO @ Sat, 27 Jun 2020 00:11:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:11:17: #1 finished! INFO @ Sat, 27 Jun 2020 00:11:17: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:11:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:11:18: #2 number of paired peaks: 70 WARNING @ Sat, 27 Jun 2020 00:11:18: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:11:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495240/SRX495240.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。