Job ID = 6508861 SRX = SRX495234 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:15:14 prefetch.2.10.7: 1) Downloading 'SRR1198739'... 2020-06-26T15:15:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:17:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:17:45 prefetch.2.10.7: 1) 'SRR1198739' was downloaded successfully Read 25111745 spots for SRR1198739/SRR1198739.sra Written 25111745 spots for SRR1198739/SRR1198739.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 485313 (1.93%) aligned 0 times 20893321 (83.20%) aligned exactly 1 time 3733111 (14.87%) aligned >1 times 98.07% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6039279 / 24626432 = 0.2452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:30:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:21: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:21: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:27: 1000000 INFO @ Sat, 27 Jun 2020 00:30:33: 2000000 INFO @ Sat, 27 Jun 2020 00:30:39: 3000000 INFO @ Sat, 27 Jun 2020 00:30:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:30:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:50: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:50: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:52: 5000000 INFO @ Sat, 27 Jun 2020 00:30:59: 1000000 INFO @ Sat, 27 Jun 2020 00:30:59: 6000000 INFO @ Sat, 27 Jun 2020 00:31:07: 2000000 INFO @ Sat, 27 Jun 2020 00:31:07: 7000000 INFO @ Sat, 27 Jun 2020 00:31:15: 8000000 INFO @ Sat, 27 Jun 2020 00:31:15: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:31:21: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:31:21: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:31:22: 9000000 INFO @ Sat, 27 Jun 2020 00:31:23: 4000000 INFO @ Sat, 27 Jun 2020 00:31:29: 1000000 INFO @ Sat, 27 Jun 2020 00:31:30: 10000000 INFO @ Sat, 27 Jun 2020 00:31:31: 5000000 INFO @ Sat, 27 Jun 2020 00:31:37: 2000000 INFO @ Sat, 27 Jun 2020 00:31:39: 11000000 INFO @ Sat, 27 Jun 2020 00:31:39: 6000000 INFO @ Sat, 27 Jun 2020 00:31:45: 3000000 INFO @ Sat, 27 Jun 2020 00:31:46: 12000000 INFO @ Sat, 27 Jun 2020 00:31:48: 7000000 INFO @ Sat, 27 Jun 2020 00:31:53: 4000000 INFO @ Sat, 27 Jun 2020 00:31:54: 13000000 INFO @ Sat, 27 Jun 2020 00:31:56: 8000000 INFO @ Sat, 27 Jun 2020 00:32:01: 5000000 INFO @ Sat, 27 Jun 2020 00:32:02: 14000000 INFO @ Sat, 27 Jun 2020 00:32:05: 9000000 INFO @ Sat, 27 Jun 2020 00:32:09: 6000000 INFO @ Sat, 27 Jun 2020 00:32:10: 15000000 INFO @ Sat, 27 Jun 2020 00:32:13: 10000000 INFO @ Sat, 27 Jun 2020 00:32:16: 7000000 INFO @ Sat, 27 Jun 2020 00:32:18: 16000000 INFO @ Sat, 27 Jun 2020 00:32:21: 11000000 INFO @ Sat, 27 Jun 2020 00:32:24: 8000000 INFO @ Sat, 27 Jun 2020 00:32:26: 17000000 INFO @ Sat, 27 Jun 2020 00:32:30: 12000000 INFO @ Sat, 27 Jun 2020 00:32:32: 9000000 INFO @ Sat, 27 Jun 2020 00:32:34: 18000000 INFO @ Sat, 27 Jun 2020 00:32:38: 13000000 INFO @ Sat, 27 Jun 2020 00:32:39: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:32:39: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:32:39: #1 total tags in treatment: 18587153 INFO @ Sat, 27 Jun 2020 00:32:39: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:32:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:32:39: #1 tags after filtering in treatment: 18587152 INFO @ Sat, 27 Jun 2020 00:32:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:32:39: #1 finished! INFO @ Sat, 27 Jun 2020 00:32:39: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:32:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:32:41: 10000000 INFO @ Sat, 27 Jun 2020 00:32:41: #2 number of paired peaks: 20 WARNING @ Sat, 27 Jun 2020 00:32:41: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:32:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:32:46: 14000000 INFO @ Sat, 27 Jun 2020 00:32:49: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:32:55: 15000000 INFO @ Sat, 27 Jun 2020 00:32:57: 12000000 INFO @ Sat, 27 Jun 2020 00:33:03: 16000000 INFO @ Sat, 27 Jun 2020 00:33:04: 13000000 INFO @ Sat, 27 Jun 2020 00:33:12: 17000000 INFO @ Sat, 27 Jun 2020 00:33:12: 14000000 INFO @ Sat, 27 Jun 2020 00:33:20: 18000000 INFO @ Sat, 27 Jun 2020 00:33:20: 15000000 INFO @ Sat, 27 Jun 2020 00:33:25: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:33:25: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:33:25: #1 total tags in treatment: 18587153 INFO @ Sat, 27 Jun 2020 00:33:25: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:33:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:33:26: #1 tags after filtering in treatment: 18587152 INFO @ Sat, 27 Jun 2020 00:33:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:33:26: #1 finished! INFO @ Sat, 27 Jun 2020 00:33:26: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:33:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:33:27: #2 number of paired peaks: 20 WARNING @ Sat, 27 Jun 2020 00:33:27: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:33:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:33:28: 16000000 INFO @ Sat, 27 Jun 2020 00:33:35: 17000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:33:41: 18000000 INFO @ Sat, 27 Jun 2020 00:33:46: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:33:46: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:33:46: #1 total tags in treatment: 18587153 INFO @ Sat, 27 Jun 2020 00:33:46: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:33:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:33:46: #1 tags after filtering in treatment: 18587152 INFO @ Sat, 27 Jun 2020 00:33:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:33:46: #1 finished! INFO @ Sat, 27 Jun 2020 00:33:46: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:33:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:33:48: #2 number of paired peaks: 20 WARNING @ Sat, 27 Jun 2020 00:33:48: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:33:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495234/SRX495234.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling