Job ID = 6508857 SRX = SRX495230 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:49:22 prefetch.2.10.7: 1) Downloading 'SRR1198735'... 2020-06-26T14:49:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:51:33 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:51:33 prefetch.2.10.7: 1) 'SRR1198735' was downloaded successfully Read 21723799 spots for SRR1198735/SRR1198735.sra Written 21723799 spots for SRR1198735/SRR1198735.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:40 21723799 reads; of these: 21723799 (100.00%) were unpaired; of these: 132697 (0.61%) aligned 0 times 19044877 (87.67%) aligned exactly 1 time 2546225 (11.72%) aligned >1 times 99.39% overall alignment rate Time searching: 00:04:40 Overall time: 00:04:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4368390 / 21591102 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:02:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:02:41: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:02:41: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:02:46: 1000000 INFO @ Sat, 27 Jun 2020 00:02:51: 2000000 INFO @ Sat, 27 Jun 2020 00:02:57: 3000000 INFO @ Sat, 27 Jun 2020 00:03:02: 4000000 INFO @ Sat, 27 Jun 2020 00:03:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:03:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:03:11: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:03:11: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:03:12: 6000000 INFO @ Sat, 27 Jun 2020 00:03:16: 1000000 INFO @ Sat, 27 Jun 2020 00:03:17: 7000000 INFO @ Sat, 27 Jun 2020 00:03:21: 2000000 INFO @ Sat, 27 Jun 2020 00:03:22: 8000000 INFO @ Sat, 27 Jun 2020 00:03:26: 3000000 INFO @ Sat, 27 Jun 2020 00:03:27: 9000000 INFO @ Sat, 27 Jun 2020 00:03:32: 4000000 INFO @ Sat, 27 Jun 2020 00:03:32: 10000000 INFO @ Sat, 27 Jun 2020 00:03:37: 5000000 INFO @ Sat, 27 Jun 2020 00:03:37: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:03:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:03:41: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:03:41: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:03:42: 6000000 INFO @ Sat, 27 Jun 2020 00:03:43: 12000000 INFO @ Sat, 27 Jun 2020 00:03:46: 1000000 INFO @ Sat, 27 Jun 2020 00:03:47: 7000000 INFO @ Sat, 27 Jun 2020 00:03:48: 13000000 INFO @ Sat, 27 Jun 2020 00:03:51: 2000000 INFO @ Sat, 27 Jun 2020 00:03:52: 8000000 INFO @ Sat, 27 Jun 2020 00:03:53: 14000000 INFO @ Sat, 27 Jun 2020 00:03:57: 3000000 INFO @ Sat, 27 Jun 2020 00:03:57: 9000000 INFO @ Sat, 27 Jun 2020 00:03:58: 15000000 INFO @ Sat, 27 Jun 2020 00:04:02: 4000000 INFO @ Sat, 27 Jun 2020 00:04:02: 10000000 INFO @ Sat, 27 Jun 2020 00:04:03: 16000000 INFO @ Sat, 27 Jun 2020 00:04:07: 5000000 INFO @ Sat, 27 Jun 2020 00:04:08: 11000000 INFO @ Sat, 27 Jun 2020 00:04:09: 17000000 INFO @ Sat, 27 Jun 2020 00:04:10: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:04:10: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:04:10: #1 total tags in treatment: 17222712 INFO @ Sat, 27 Jun 2020 00:04:10: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:04:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:04:11: #1 tags after filtering in treatment: 17222698 INFO @ Sat, 27 Jun 2020 00:04:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:04:11: #1 finished! INFO @ Sat, 27 Jun 2020 00:04:11: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:04:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:04:12: #2 number of paired peaks: 5 WARNING @ Sat, 27 Jun 2020 00:04:12: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:04:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:04:13: 6000000 INFO @ Sat, 27 Jun 2020 00:04:13: 12000000 INFO @ Sat, 27 Jun 2020 00:04:18: 7000000 INFO @ Sat, 27 Jun 2020 00:04:18: 13000000 INFO @ Sat, 27 Jun 2020 00:04:23: 8000000 INFO @ Sat, 27 Jun 2020 00:04:23: 14000000 INFO @ Sat, 27 Jun 2020 00:04:29: 9000000 INFO @ Sat, 27 Jun 2020 00:04:29: 15000000 INFO @ Sat, 27 Jun 2020 00:04:34: 10000000 INFO @ Sat, 27 Jun 2020 00:04:34: 16000000 INFO @ Sat, 27 Jun 2020 00:04:39: 11000000 INFO @ Sat, 27 Jun 2020 00:04:40: 17000000 INFO @ Sat, 27 Jun 2020 00:04:41: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:04:41: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:04:41: #1 total tags in treatment: 17222712 INFO @ Sat, 27 Jun 2020 00:04:41: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:04:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:04:42: #1 tags after filtering in treatment: 17222698 INFO @ Sat, 27 Jun 2020 00:04:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:04:42: #1 finished! INFO @ Sat, 27 Jun 2020 00:04:42: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:04:43: #2 number of paired peaks: 5 WARNING @ Sat, 27 Jun 2020 00:04:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:04:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:04:45: 12000000 INFO @ Sat, 27 Jun 2020 00:04:50: 13000000 INFO @ Sat, 27 Jun 2020 00:04:55: 14000000 INFO @ Sat, 27 Jun 2020 00:05:01: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:05:07: 16000000 INFO @ Sat, 27 Jun 2020 00:05:13: 17000000 INFO @ Sat, 27 Jun 2020 00:05:14: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:05:14: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:05:14: #1 total tags in treatment: 17222712 INFO @ Sat, 27 Jun 2020 00:05:14: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:05:15: #1 tags after filtering in treatment: 17222698 INFO @ Sat, 27 Jun 2020 00:05:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:05:15: #1 finished! INFO @ Sat, 27 Jun 2020 00:05:15: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:05:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:05:16: #2 number of paired peaks: 5 WARNING @ Sat, 27 Jun 2020 00:05:16: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:05:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495230/SRX495230.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。