Job ID = 6508854 SRX = SRX495227 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:24:36 prefetch.2.10.7: 1) Downloading 'SRR1198732'... 2020-06-26T15:24:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:25:51 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:25:52 prefetch.2.10.7: 'SRR1198732' is valid 2020-06-26T15:25:52 prefetch.2.10.7: 1) 'SRR1198732' was downloaded successfully Read 15013137 spots for SRR1198732/SRR1198732.sra Written 15013137 spots for SRR1198732/SRR1198732.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:37 15013137 reads; of these: 15013137 (100.00%) were unpaired; of these: 4468442 (29.76%) aligned 0 times 9168724 (61.07%) aligned exactly 1 time 1375971 (9.17%) aligned >1 times 70.24% overall alignment rate Time searching: 00:02:37 Overall time: 00:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1311890 / 10544695 = 0.1244 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:32:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:32:23: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:32:23: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:32:29: 1000000 INFO @ Sat, 27 Jun 2020 00:32:35: 2000000 INFO @ Sat, 27 Jun 2020 00:32:41: 3000000 INFO @ Sat, 27 Jun 2020 00:32:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:32:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:32:53: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:32:53: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:32:53: 5000000 INFO @ Sat, 27 Jun 2020 00:33:00: 1000000 INFO @ Sat, 27 Jun 2020 00:33:00: 6000000 INFO @ Sat, 27 Jun 2020 00:33:07: 2000000 INFO @ Sat, 27 Jun 2020 00:33:07: 7000000 INFO @ Sat, 27 Jun 2020 00:33:13: 3000000 INFO @ Sat, 27 Jun 2020 00:33:14: 8000000 INFO @ Sat, 27 Jun 2020 00:33:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:33:21: 9000000 INFO @ Sat, 27 Jun 2020 00:33:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:33:23: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:33:23: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:33:23: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:33:23: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:33:23: #1 total tags in treatment: 9232805 INFO @ Sat, 27 Jun 2020 00:33:23: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:33:23: #1 tags after filtering in treatment: 9232769 INFO @ Sat, 27 Jun 2020 00:33:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:33:23: #1 finished! INFO @ Sat, 27 Jun 2020 00:33:23: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:33:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:33:24: #2 number of paired peaks: 30 WARNING @ Sat, 27 Jun 2020 00:33:24: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:33:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:33:26: 5000000 INFO @ Sat, 27 Jun 2020 00:33:29: 1000000 INFO @ Sat, 27 Jun 2020 00:33:33: 6000000 INFO @ Sat, 27 Jun 2020 00:33:35: 2000000 INFO @ Sat, 27 Jun 2020 00:33:39: 7000000 INFO @ Sat, 27 Jun 2020 00:33:42: 3000000 INFO @ Sat, 27 Jun 2020 00:33:46: 8000000 INFO @ Sat, 27 Jun 2020 00:33:48: 4000000 INFO @ Sat, 27 Jun 2020 00:33:52: 9000000 INFO @ Sat, 27 Jun 2020 00:33:53: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:33:53: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:33:53: #1 total tags in treatment: 9232805 INFO @ Sat, 27 Jun 2020 00:33:53: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:33:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:33:54: 5000000 INFO @ Sat, 27 Jun 2020 00:33:54: #1 tags after filtering in treatment: 9232769 INFO @ Sat, 27 Jun 2020 00:33:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:33:54: #1 finished! INFO @ Sat, 27 Jun 2020 00:33:54: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:33:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:33:54: #2 number of paired peaks: 30 WARNING @ Sat, 27 Jun 2020 00:33:54: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:33:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:33:59: 6000000 INFO @ Sat, 27 Jun 2020 00:34:05: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:34:12: 8000000 INFO @ Sat, 27 Jun 2020 00:34:18: 9000000 INFO @ Sat, 27 Jun 2020 00:34:19: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:34:19: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:34:19: #1 total tags in treatment: 9232805 INFO @ Sat, 27 Jun 2020 00:34:19: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:34:20: #1 tags after filtering in treatment: 9232769 INFO @ Sat, 27 Jun 2020 00:34:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:34:20: #1 finished! INFO @ Sat, 27 Jun 2020 00:34:20: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:34:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:34:20: #2 number of paired peaks: 30 WARNING @ Sat, 27 Jun 2020 00:34:20: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:34:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495227/SRX495227.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。