Job ID = 6508853 SRX = SRX495226 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:10:43 prefetch.2.10.7: 1) Downloading 'SRR1198731'... 2020-06-26T15:10:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:13:17 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:13:17 prefetch.2.10.7: 1) 'SRR1198731' was downloaded successfully Read 25111745 spots for SRR1198731/SRR1198731.sra Written 25111745 spots for SRR1198731/SRR1198731.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:35 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 485309 (1.93%) aligned 0 times 20893319 (83.20%) aligned exactly 1 time 3733117 (14.87%) aligned >1 times 98.07% overall alignment rate Time searching: 00:05:35 Overall time: 00:05:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6038448 / 24626436 = 0.2452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:25:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:25:49: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:25:49: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:25:55: 1000000 INFO @ Sat, 27 Jun 2020 00:26:01: 2000000 INFO @ Sat, 27 Jun 2020 00:26:07: 3000000 INFO @ Sat, 27 Jun 2020 00:26:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:26:18: 5000000 INFO @ Sat, 27 Jun 2020 00:26:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:26:19: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:26:19: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:26:25: 6000000 INFO @ Sat, 27 Jun 2020 00:26:25: 1000000 INFO @ Sat, 27 Jun 2020 00:26:31: 7000000 INFO @ Sat, 27 Jun 2020 00:26:32: 2000000 INFO @ Sat, 27 Jun 2020 00:26:38: 8000000 INFO @ Sat, 27 Jun 2020 00:26:39: 3000000 INFO @ Sat, 27 Jun 2020 00:26:45: 9000000 INFO @ Sat, 27 Jun 2020 00:26:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:26:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:26:49: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:26:49: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:26:51: 10000000 INFO @ Sat, 27 Jun 2020 00:26:52: 5000000 INFO @ Sat, 27 Jun 2020 00:26:56: 1000000 INFO @ Sat, 27 Jun 2020 00:26:58: 11000000 INFO @ Sat, 27 Jun 2020 00:26:59: 6000000 INFO @ Sat, 27 Jun 2020 00:27:03: 2000000 INFO @ Sat, 27 Jun 2020 00:27:05: 12000000 INFO @ Sat, 27 Jun 2020 00:27:06: 7000000 INFO @ Sat, 27 Jun 2020 00:27:10: 3000000 INFO @ Sat, 27 Jun 2020 00:27:12: 13000000 INFO @ Sat, 27 Jun 2020 00:27:13: 8000000 INFO @ Sat, 27 Jun 2020 00:27:17: 4000000 INFO @ Sat, 27 Jun 2020 00:27:19: 14000000 INFO @ Sat, 27 Jun 2020 00:27:19: 9000000 INFO @ Sat, 27 Jun 2020 00:27:23: 5000000 INFO @ Sat, 27 Jun 2020 00:27:25: 15000000 INFO @ Sat, 27 Jun 2020 00:27:26: 10000000 INFO @ Sat, 27 Jun 2020 00:27:30: 6000000 INFO @ Sat, 27 Jun 2020 00:27:33: 16000000 INFO @ Sat, 27 Jun 2020 00:27:33: 11000000 INFO @ Sat, 27 Jun 2020 00:27:37: 7000000 INFO @ Sat, 27 Jun 2020 00:27:40: 17000000 INFO @ Sat, 27 Jun 2020 00:27:40: 12000000 INFO @ Sat, 27 Jun 2020 00:27:44: 8000000 INFO @ Sat, 27 Jun 2020 00:27:47: 18000000 INFO @ Sat, 27 Jun 2020 00:27:47: 13000000 INFO @ Sat, 27 Jun 2020 00:27:51: 9000000 INFO @ Sat, 27 Jun 2020 00:27:51: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:27:51: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:27:51: #1 total tags in treatment: 18587988 INFO @ Sat, 27 Jun 2020 00:27:51: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:27:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:27:51: #1 tags after filtering in treatment: 18587985 INFO @ Sat, 27 Jun 2020 00:27:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:27:51: #1 finished! INFO @ Sat, 27 Jun 2020 00:27:51: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:27:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:27:53: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:27:53: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:27:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:27:53: 14000000 INFO @ Sat, 27 Jun 2020 00:27:57: 10000000 INFO @ Sat, 27 Jun 2020 00:28:00: 15000000 INFO @ Sat, 27 Jun 2020 00:28:04: 11000000 INFO @ Sat, 27 Jun 2020 00:28:07: 16000000 INFO @ Sat, 27 Jun 2020 00:28:11: 12000000 INFO @ Sat, 27 Jun 2020 00:28:14: 17000000 INFO @ Sat, 27 Jun 2020 00:28:18: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:28:21: 18000000 INFO @ Sat, 27 Jun 2020 00:28:24: 14000000 INFO @ Sat, 27 Jun 2020 00:28:25: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:28:25: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:28:25: #1 total tags in treatment: 18587988 INFO @ Sat, 27 Jun 2020 00:28:25: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:28:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:28:25: #1 tags after filtering in treatment: 18587985 INFO @ Sat, 27 Jun 2020 00:28:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:28:25: #1 finished! INFO @ Sat, 27 Jun 2020 00:28:25: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:28:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:28:27: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:28:27: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:28:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:28:30: 15000000 INFO @ Sat, 27 Jun 2020 00:28:37: 16000000 INFO @ Sat, 27 Jun 2020 00:28:43: 17000000 INFO @ Sat, 27 Jun 2020 00:28:49: 18000000 INFO @ Sat, 27 Jun 2020 00:28:52: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:28:52: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:28:52: #1 total tags in treatment: 18587988 INFO @ Sat, 27 Jun 2020 00:28:52: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:28:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:28:53: #1 tags after filtering in treatment: 18587985 INFO @ Sat, 27 Jun 2020 00:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:28:53: #1 finished! INFO @ Sat, 27 Jun 2020 00:28:53: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:28:54: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:28:54: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:28:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495226/SRX495226.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。