Job ID = 6508852 SRX = SRX495225 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:08:29 prefetch.2.10.7: 1) Downloading 'SRR1198730'... 2020-06-26T15:08:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:10:44 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:10:44 prefetch.2.10.7: 1) 'SRR1198730' was downloaded successfully Read 25111745 spots for SRR1198730/SRR1198730.sra Written 25111745 spots for SRR1198730/SRR1198730.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:11 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 485269 (1.93%) aligned 0 times 20893318 (83.20%) aligned exactly 1 time 3733158 (14.87%) aligned >1 times 98.07% overall alignment rate Time searching: 00:05:11 Overall time: 00:05:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6038522 / 24626476 = 0.2452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:23:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:23:59: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:23:59: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:24:06: 1000000 INFO @ Sat, 27 Jun 2020 00:24:12: 2000000 INFO @ Sat, 27 Jun 2020 00:24:18: 3000000 INFO @ Sat, 27 Jun 2020 00:24:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:24:29: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:24:29: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:24:31: 5000000 INFO @ Sat, 27 Jun 2020 00:24:35: 1000000 INFO @ Sat, 27 Jun 2020 00:24:37: 6000000 INFO @ Sat, 27 Jun 2020 00:24:41: 2000000 INFO @ Sat, 27 Jun 2020 00:24:44: 7000000 INFO @ Sat, 27 Jun 2020 00:24:48: 3000000 INFO @ Sat, 27 Jun 2020 00:24:50: 8000000 INFO @ Sat, 27 Jun 2020 00:24:54: 4000000 INFO @ Sat, 27 Jun 2020 00:24:56: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:24:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:24:59: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:24:59: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:25:00: 5000000 INFO @ Sat, 27 Jun 2020 00:25:03: 10000000 INFO @ Sat, 27 Jun 2020 00:25:06: 6000000 INFO @ Sat, 27 Jun 2020 00:25:07: 1000000 INFO @ Sat, 27 Jun 2020 00:25:11: 11000000 INFO @ Sat, 27 Jun 2020 00:25:12: 7000000 INFO @ Sat, 27 Jun 2020 00:25:14: 2000000 INFO @ Sat, 27 Jun 2020 00:25:18: 12000000 INFO @ Sat, 27 Jun 2020 00:25:19: 8000000 INFO @ Sat, 27 Jun 2020 00:25:22: 3000000 INFO @ Sat, 27 Jun 2020 00:25:25: 9000000 INFO @ Sat, 27 Jun 2020 00:25:26: 13000000 INFO @ Sat, 27 Jun 2020 00:25:29: 4000000 INFO @ Sat, 27 Jun 2020 00:25:31: 10000000 INFO @ Sat, 27 Jun 2020 00:25:33: 14000000 INFO @ Sat, 27 Jun 2020 00:25:36: 5000000 INFO @ Sat, 27 Jun 2020 00:25:37: 11000000 INFO @ Sat, 27 Jun 2020 00:25:40: 15000000 INFO @ Sat, 27 Jun 2020 00:25:43: 6000000 INFO @ Sat, 27 Jun 2020 00:25:44: 12000000 INFO @ Sat, 27 Jun 2020 00:25:47: 16000000 INFO @ Sat, 27 Jun 2020 00:25:50: 13000000 INFO @ Sat, 27 Jun 2020 00:25:50: 7000000 INFO @ Sat, 27 Jun 2020 00:25:54: 17000000 INFO @ Sat, 27 Jun 2020 00:25:57: 14000000 INFO @ Sat, 27 Jun 2020 00:25:58: 8000000 INFO @ Sat, 27 Jun 2020 00:26:01: 18000000 INFO @ Sat, 27 Jun 2020 00:26:03: 15000000 INFO @ Sat, 27 Jun 2020 00:26:04: 9000000 INFO @ Sat, 27 Jun 2020 00:26:06: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:26:06: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:26:06: #1 total tags in treatment: 18587954 INFO @ Sat, 27 Jun 2020 00:26:06: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:26:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:26:07: #1 tags after filtering in treatment: 18587953 INFO @ Sat, 27 Jun 2020 00:26:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:26:07: #1 finished! INFO @ Sat, 27 Jun 2020 00:26:07: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:26:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:26:08: #2 number of paired peaks: 16 WARNING @ Sat, 27 Jun 2020 00:26:08: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:26:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:26:10: 16000000 INFO @ Sat, 27 Jun 2020 00:26:11: 10000000 INFO @ Sat, 27 Jun 2020 00:26:17: 17000000 INFO @ Sat, 27 Jun 2020 00:26:17: 11000000 INFO @ Sat, 27 Jun 2020 00:26:23: 18000000 INFO @ Sat, 27 Jun 2020 00:26:24: 12000000 INFO @ Sat, 27 Jun 2020 00:26:27: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:26:27: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:26:27: #1 total tags in treatment: 18587954 INFO @ Sat, 27 Jun 2020 00:26:27: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:26:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:26:28: #1 tags after filtering in treatment: 18587953 INFO @ Sat, 27 Jun 2020 00:26:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:26:28: #1 finished! INFO @ Sat, 27 Jun 2020 00:26:28: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:26:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:26:29: #2 number of paired peaks: 16 WARNING @ Sat, 27 Jun 2020 00:26:29: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:26:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:26:30: 13000000 INFO @ Sat, 27 Jun 2020 00:26:36: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:26:42: 15000000 INFO @ Sat, 27 Jun 2020 00:26:49: 16000000 INFO @ Sat, 27 Jun 2020 00:26:55: 17000000 INFO @ Sat, 27 Jun 2020 00:27:01: 18000000 INFO @ Sat, 27 Jun 2020 00:27:05: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:27:05: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:27:05: #1 total tags in treatment: 18587954 INFO @ Sat, 27 Jun 2020 00:27:05: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:27:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:27:06: #1 tags after filtering in treatment: 18587953 INFO @ Sat, 27 Jun 2020 00:27:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:27:06: #1 finished! INFO @ Sat, 27 Jun 2020 00:27:06: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:27:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:27:07: #2 number of paired peaks: 16 WARNING @ Sat, 27 Jun 2020 00:27:07: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:27:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495225/SRX495225.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。