Job ID = 6508850 SRX = SRX495223 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:53:37 prefetch.2.10.7: 1) Downloading 'SRR1198728'... 2020-06-26T14:53:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:55:20 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:55:20 prefetch.2.10.7: 1) 'SRR1198728' was downloaded successfully Read 23604057 spots for SRR1198728/SRR1198728.sra Written 23604057 spots for SRR1198728/SRR1198728.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 23604057 reads; of these: 23604057 (100.00%) were unpaired; of these: 1834764 (7.77%) aligned 0 times 15708872 (66.55%) aligned exactly 1 time 6060421 (25.68%) aligned >1 times 92.23% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6245355 / 21769293 = 0.2869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:06:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:06:35: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:06:35: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:06:41: 1000000 INFO @ Sat, 27 Jun 2020 00:06:46: 2000000 INFO @ Sat, 27 Jun 2020 00:06:51: 3000000 INFO @ Sat, 27 Jun 2020 00:06:57: 4000000 INFO @ Sat, 27 Jun 2020 00:07:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:07:05: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:07:05: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:07:07: 6000000 INFO @ Sat, 27 Jun 2020 00:07:10: 1000000 INFO @ Sat, 27 Jun 2020 00:07:13: 7000000 INFO @ Sat, 27 Jun 2020 00:07:15: 2000000 INFO @ Sat, 27 Jun 2020 00:07:18: 8000000 INFO @ Sat, 27 Jun 2020 00:07:20: 3000000 INFO @ Sat, 27 Jun 2020 00:07:24: 9000000 INFO @ Sat, 27 Jun 2020 00:07:25: 4000000 INFO @ Sat, 27 Jun 2020 00:07:29: 10000000 INFO @ Sat, 27 Jun 2020 00:07:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:07:34: 11000000 INFO @ Sat, 27 Jun 2020 00:07:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:07:35: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:07:35: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:07:35: 6000000 INFO @ Sat, 27 Jun 2020 00:07:40: 12000000 INFO @ Sat, 27 Jun 2020 00:07:40: 1000000 INFO @ Sat, 27 Jun 2020 00:07:40: 7000000 INFO @ Sat, 27 Jun 2020 00:07:45: 2000000 INFO @ Sat, 27 Jun 2020 00:07:45: 8000000 INFO @ Sat, 27 Jun 2020 00:07:45: 13000000 INFO @ Sat, 27 Jun 2020 00:07:50: 3000000 INFO @ Sat, 27 Jun 2020 00:07:50: 9000000 INFO @ Sat, 27 Jun 2020 00:07:51: 14000000 INFO @ Sat, 27 Jun 2020 00:07:55: 4000000 INFO @ Sat, 27 Jun 2020 00:07:55: 10000000 INFO @ Sat, 27 Jun 2020 00:07:56: 15000000 INFO @ Sat, 27 Jun 2020 00:08:00: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:08:00: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:08:00: #1 total tags in treatment: 15523938 INFO @ Sat, 27 Jun 2020 00:08:00: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:08:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:08:00: 11000000 INFO @ Sat, 27 Jun 2020 00:08:00: 5000000 INFO @ Sat, 27 Jun 2020 00:08:00: #1 tags after filtering in treatment: 15523871 INFO @ Sat, 27 Jun 2020 00:08:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:08:00: #1 finished! INFO @ Sat, 27 Jun 2020 00:08:00: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:08:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:08:01: #2 number of paired peaks: 97 WARNING @ Sat, 27 Jun 2020 00:08:01: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:08:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:08:05: 12000000 INFO @ Sat, 27 Jun 2020 00:08:05: 6000000 INFO @ Sat, 27 Jun 2020 00:08:10: 7000000 INFO @ Sat, 27 Jun 2020 00:08:10: 13000000 INFO @ Sat, 27 Jun 2020 00:08:15: 8000000 INFO @ Sat, 27 Jun 2020 00:08:15: 14000000 INFO @ Sat, 27 Jun 2020 00:08:20: 9000000 INFO @ Sat, 27 Jun 2020 00:08:21: 15000000 INFO @ Sat, 27 Jun 2020 00:08:24: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:08:24: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:08:24: #1 total tags in treatment: 15523938 INFO @ Sat, 27 Jun 2020 00:08:24: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:08:24: #1 tags after filtering in treatment: 15523871 INFO @ Sat, 27 Jun 2020 00:08:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:08:24: #1 finished! INFO @ Sat, 27 Jun 2020 00:08:24: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:08:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:08:25: 10000000 INFO @ Sat, 27 Jun 2020 00:08:25: #2 number of paired peaks: 97 WARNING @ Sat, 27 Jun 2020 00:08:25: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:08:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:08:30: 11000000 INFO @ Sat, 27 Jun 2020 00:08:34: 12000000 INFO @ Sat, 27 Jun 2020 00:08:40: 13000000 INFO @ Sat, 27 Jun 2020 00:08:44: 14000000 INFO @ Sat, 27 Jun 2020 00:08:49: 15000000 INFO @ Sat, 27 Jun 2020 00:08:52: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:08:52: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:08:52: #1 total tags in treatment: 15523938 INFO @ Sat, 27 Jun 2020 00:08:52: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:08:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:08:53: #1 tags after filtering in treatment: 15523871 INFO @ Sat, 27 Jun 2020 00:08:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:08:53: #1 finished! INFO @ Sat, 27 Jun 2020 00:08:53: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:08:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:08:54: #2 number of paired peaks: 97 WARNING @ Sat, 27 Jun 2020 00:08:54: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:08:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495223/SRX495223.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。