Job ID = 6508836 SRX = SRX495211 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T14:55:38 prefetch.2.10.7: 1) Downloading 'SRR1198716'... 2020-06-26T14:55:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T14:57:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T14:57:45 prefetch.2.10.7: 1) 'SRR1198716' was downloaded successfully Read 22529054 spots for SRR1198716/SRR1198716.sra Written 22529054 spots for SRR1198716/SRR1198716.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:31 22529054 reads; of these: 22529054 (100.00%) were unpaired; of these: 4442519 (19.72%) aligned 0 times 15661120 (69.52%) aligned exactly 1 time 2425415 (10.77%) aligned >1 times 80.28% overall alignment rate Time searching: 00:04:31 Overall time: 00:04:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4212312 / 18086535 = 0.2329 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:07:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:07:55: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:07:55: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:08:01: 1000000 INFO @ Sat, 27 Jun 2020 00:08:07: 2000000 INFO @ Sat, 27 Jun 2020 00:08:13: 3000000 INFO @ Sat, 27 Jun 2020 00:08:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:08:24: 5000000 INFO @ Sat, 27 Jun 2020 00:08:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:08:25: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:08:25: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:08:30: 6000000 INFO @ Sat, 27 Jun 2020 00:08:31: 1000000 INFO @ Sat, 27 Jun 2020 00:08:37: 7000000 INFO @ Sat, 27 Jun 2020 00:08:38: 2000000 INFO @ Sat, 27 Jun 2020 00:08:44: 8000000 INFO @ Sat, 27 Jun 2020 00:08:44: 3000000 INFO @ Sat, 27 Jun 2020 00:08:50: 9000000 INFO @ Sat, 27 Jun 2020 00:08:51: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:08:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:08:55: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:08:55: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:08:57: 10000000 INFO @ Sat, 27 Jun 2020 00:08:57: 5000000 INFO @ Sat, 27 Jun 2020 00:09:02: 1000000 INFO @ Sat, 27 Jun 2020 00:09:03: 11000000 INFO @ Sat, 27 Jun 2020 00:09:04: 6000000 INFO @ Sat, 27 Jun 2020 00:09:09: 2000000 INFO @ Sat, 27 Jun 2020 00:09:10: 12000000 INFO @ Sat, 27 Jun 2020 00:09:11: 7000000 INFO @ Sat, 27 Jun 2020 00:09:16: 3000000 INFO @ Sat, 27 Jun 2020 00:09:17: 13000000 INFO @ Sat, 27 Jun 2020 00:09:17: 8000000 INFO @ Sat, 27 Jun 2020 00:09:22: 4000000 INFO @ Sat, 27 Jun 2020 00:09:23: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:09:23: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:09:23: #1 total tags in treatment: 13874223 INFO @ Sat, 27 Jun 2020 00:09:23: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:09:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:09:24: #1 tags after filtering in treatment: 13874216 INFO @ Sat, 27 Jun 2020 00:09:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:09:24: #1 finished! INFO @ Sat, 27 Jun 2020 00:09:24: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:09:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:09:24: 9000000 INFO @ Sat, 27 Jun 2020 00:09:25: #2 number of paired peaks: 31 WARNING @ Sat, 27 Jun 2020 00:09:25: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:09:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:09:29: 5000000 INFO @ Sat, 27 Jun 2020 00:09:31: 10000000 INFO @ Sat, 27 Jun 2020 00:09:36: 6000000 INFO @ Sat, 27 Jun 2020 00:09:37: 11000000 INFO @ Sat, 27 Jun 2020 00:09:43: 7000000 INFO @ Sat, 27 Jun 2020 00:09:44: 12000000 INFO @ Sat, 27 Jun 2020 00:09:49: 8000000 INFO @ Sat, 27 Jun 2020 00:09:51: 13000000 INFO @ Sat, 27 Jun 2020 00:09:56: 9000000 INFO @ Sat, 27 Jun 2020 00:09:57: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:09:57: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:09:57: #1 total tags in treatment: 13874223 INFO @ Sat, 27 Jun 2020 00:09:57: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:09:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:09:58: #1 tags after filtering in treatment: 13874216 INFO @ Sat, 27 Jun 2020 00:09:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:09:58: #1 finished! INFO @ Sat, 27 Jun 2020 00:09:58: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:09:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:09:59: #2 number of paired peaks: 31 WARNING @ Sat, 27 Jun 2020 00:09:59: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:09:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:10:03: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:10:09: 11000000 INFO @ Sat, 27 Jun 2020 00:10:16: 12000000 INFO @ Sat, 27 Jun 2020 00:10:22: 13000000 INFO @ Sat, 27 Jun 2020 00:10:28: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:10:28: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:10:28: #1 total tags in treatment: 13874223 INFO @ Sat, 27 Jun 2020 00:10:28: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:10:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:10:29: #1 tags after filtering in treatment: 13874216 INFO @ Sat, 27 Jun 2020 00:10:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:10:29: #1 finished! INFO @ Sat, 27 Jun 2020 00:10:29: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:10:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:10:30: #2 number of paired peaks: 31 WARNING @ Sat, 27 Jun 2020 00:10:30: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:10:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495211/SRX495211.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。