Job ID = 6458072 SRX = SRX495199 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:13:04 prefetch.2.10.7: 1) Downloading 'SRR1198704'... 2020-06-21T12:13:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:16:22 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:16:22 prefetch.2.10.7: 1) 'SRR1198704' was downloaded successfully Read 20576294 spots for SRR1198704/SRR1198704.sra Written 20576294 spots for SRR1198704/SRR1198704.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:13 20576294 reads; of these: 20576294 (100.00%) were unpaired; of these: 1068590 (5.19%) aligned 0 times 16280248 (79.12%) aligned exactly 1 time 3227456 (15.69%) aligned >1 times 94.81% overall alignment rate Time searching: 00:04:13 Overall time: 00:04:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5380968 / 19507704 = 0.2758 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:25:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:25:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:25:51: 1000000 INFO @ Sun, 21 Jun 2020 21:25:56: 2000000 INFO @ Sun, 21 Jun 2020 21:26:02: 3000000 INFO @ Sun, 21 Jun 2020 21:26:06: 4000000 INFO @ Sun, 21 Jun 2020 21:26:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:16: 6000000 INFO @ Sun, 21 Jun 2020 21:26:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:21: 7000000 INFO @ Sun, 21 Jun 2020 21:26:21: 1000000 INFO @ Sun, 21 Jun 2020 21:26:26: 8000000 INFO @ Sun, 21 Jun 2020 21:26:26: 2000000 INFO @ Sun, 21 Jun 2020 21:26:31: 9000000 INFO @ Sun, 21 Jun 2020 21:26:31: 3000000 INFO @ Sun, 21 Jun 2020 21:26:36: 10000000 INFO @ Sun, 21 Jun 2020 21:26:36: 4000000 INFO @ Sun, 21 Jun 2020 21:26:41: 11000000 INFO @ Sun, 21 Jun 2020 21:26:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:46: 6000000 INFO @ Sun, 21 Jun 2020 21:26:46: 12000000 INFO @ Sun, 21 Jun 2020 21:26:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:51: 13000000 INFO @ Sun, 21 Jun 2020 21:26:51: 7000000 INFO @ Sun, 21 Jun 2020 21:26:51: 1000000 INFO @ Sun, 21 Jun 2020 21:26:56: 8000000 INFO @ Sun, 21 Jun 2020 21:26:56: 14000000 INFO @ Sun, 21 Jun 2020 21:26:56: 2000000 INFO @ Sun, 21 Jun 2020 21:26:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:26:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:26:56: #1 total tags in treatment: 14126736 INFO @ Sun, 21 Jun 2020 21:26:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:57: #1 tags after filtering in treatment: 14126735 INFO @ Sun, 21 Jun 2020 21:26:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:57: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:58: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 21:26:58: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:27:01: 9000000 INFO @ Sun, 21 Jun 2020 21:27:01: 3000000 INFO @ Sun, 21 Jun 2020 21:27:06: 10000000 INFO @ Sun, 21 Jun 2020 21:27:06: 4000000 INFO @ Sun, 21 Jun 2020 21:27:10: 11000000 INFO @ Sun, 21 Jun 2020 21:27:10: 5000000 INFO @ Sun, 21 Jun 2020 21:27:15: 6000000 INFO @ Sun, 21 Jun 2020 21:27:16: 12000000 INFO @ Sun, 21 Jun 2020 21:27:20: 7000000 INFO @ Sun, 21 Jun 2020 21:27:21: 13000000 INFO @ Sun, 21 Jun 2020 21:27:25: 8000000 INFO @ Sun, 21 Jun 2020 21:27:26: 14000000 INFO @ Sun, 21 Jun 2020 21:27:26: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:27:26: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:27:26: #1 total tags in treatment: 14126736 INFO @ Sun, 21 Jun 2020 21:27:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:27:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:27: #1 tags after filtering in treatment: 14126735 INFO @ Sun, 21 Jun 2020 21:27:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:27:28: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 21:27:28: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:27:30: 9000000 INFO @ Sun, 21 Jun 2020 21:27:35: 10000000 INFO @ Sun, 21 Jun 2020 21:27:39: 11000000 INFO @ Sun, 21 Jun 2020 21:27:44: 12000000 INFO @ Sun, 21 Jun 2020 21:27:49: 13000000 INFO @ Sun, 21 Jun 2020 21:27:54: 14000000 INFO @ Sun, 21 Jun 2020 21:27:55: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:27:55: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:27:55: #1 total tags in treatment: 14126736 INFO @ Sun, 21 Jun 2020 21:27:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:27:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:55: #1 tags after filtering in treatment: 14126735 INFO @ Sun, 21 Jun 2020 21:27:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:55: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:27:56: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 21:27:56: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495199/SRX495199.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。