Job ID = 6458068 SRX = SRX495198 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:12:34 prefetch.2.10.7: 1) Downloading 'SRR1198703'... 2020-06-21T12:12:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:14:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:14:50 prefetch.2.10.7: 1) 'SRR1198703' was downloaded successfully Read 21457757 spots for SRR1198703/SRR1198703.sra Written 21457757 spots for SRR1198703/SRR1198703.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:02 21457757 reads; of these: 21457757 (100.00%) were unpaired; of these: 542448 (2.53%) aligned 0 times 16529283 (77.03%) aligned exactly 1 time 4386026 (20.44%) aligned >1 times 97.47% overall alignment rate Time searching: 00:05:02 Overall time: 00:05:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4812455 / 20915309 = 0.2301 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:25:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:25:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:25:52: 1000000 INFO @ Sun, 21 Jun 2020 21:25:58: 2000000 INFO @ Sun, 21 Jun 2020 21:26:04: 3000000 INFO @ Sun, 21 Jun 2020 21:26:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:16: 5000000 INFO @ Sun, 21 Jun 2020 21:26:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:22: 1000000 INFO @ Sun, 21 Jun 2020 21:26:22: 6000000 INFO @ Sun, 21 Jun 2020 21:26:27: 2000000 INFO @ Sun, 21 Jun 2020 21:26:28: 7000000 INFO @ Sun, 21 Jun 2020 21:26:33: 3000000 INFO @ Sun, 21 Jun 2020 21:26:35: 8000000 INFO @ Sun, 21 Jun 2020 21:26:39: 4000000 INFO @ Sun, 21 Jun 2020 21:26:41: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:44: 5000000 INFO @ Sun, 21 Jun 2020 21:26:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:47: 10000000 INFO @ Sun, 21 Jun 2020 21:26:50: 6000000 INFO @ Sun, 21 Jun 2020 21:26:52: 1000000 INFO @ Sun, 21 Jun 2020 21:26:54: 11000000 INFO @ Sun, 21 Jun 2020 21:26:55: 7000000 INFO @ Sun, 21 Jun 2020 21:26:59: 2000000 INFO @ Sun, 21 Jun 2020 21:27:00: 12000000 INFO @ Sun, 21 Jun 2020 21:27:01: 8000000 INFO @ Sun, 21 Jun 2020 21:27:05: 3000000 INFO @ Sun, 21 Jun 2020 21:27:07: 9000000 INFO @ Sun, 21 Jun 2020 21:27:07: 13000000 INFO @ Sun, 21 Jun 2020 21:27:12: 4000000 INFO @ Sun, 21 Jun 2020 21:27:13: 10000000 INFO @ Sun, 21 Jun 2020 21:27:14: 14000000 INFO @ Sun, 21 Jun 2020 21:27:18: 5000000 INFO @ Sun, 21 Jun 2020 21:27:18: 11000000 INFO @ Sun, 21 Jun 2020 21:27:21: 15000000 INFO @ Sun, 21 Jun 2020 21:27:24: 12000000 INFO @ Sun, 21 Jun 2020 21:27:24: 6000000 INFO @ Sun, 21 Jun 2020 21:27:27: 16000000 INFO @ Sun, 21 Jun 2020 21:27:28: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:27:28: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:27:28: #1 total tags in treatment: 16102854 INFO @ Sun, 21 Jun 2020 21:27:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:27:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:28: #1 tags after filtering in treatment: 16102853 INFO @ Sun, 21 Jun 2020 21:27:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:27:29: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 21:27:29: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:29: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 21:27:30: 13000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:27:31: 7000000 INFO @ Sun, 21 Jun 2020 21:27:36: 14000000 INFO @ Sun, 21 Jun 2020 21:27:37: 8000000 INFO @ Sun, 21 Jun 2020 21:27:41: 15000000 INFO @ Sun, 21 Jun 2020 21:27:43: 9000000 INFO @ Sun, 21 Jun 2020 21:27:47: 16000000 INFO @ Sun, 21 Jun 2020 21:27:48: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:27:48: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:27:48: #1 total tags in treatment: 16102854 INFO @ Sun, 21 Jun 2020 21:27:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:27:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:49: #1 tags after filtering in treatment: 16102853 INFO @ Sun, 21 Jun 2020 21:27:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:27:49: 10000000 INFO @ Sun, 21 Jun 2020 21:27:50: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 21:27:50: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:27:55: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:28:02: 12000000 INFO @ Sun, 21 Jun 2020 21:28:07: 13000000 INFO @ Sun, 21 Jun 2020 21:28:14: 14000000 INFO @ Sun, 21 Jun 2020 21:28:20: 15000000 INFO @ Sun, 21 Jun 2020 21:28:26: 16000000 INFO @ Sun, 21 Jun 2020 21:28:26: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:28:26: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:28:26: #1 total tags in treatment: 16102854 INFO @ Sun, 21 Jun 2020 21:28:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:28:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:28:27: #1 tags after filtering in treatment: 16102853 INFO @ Sun, 21 Jun 2020 21:28:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:28:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:28:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:28:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:28:28: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 21:28:28: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:28:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495198/SRX495198.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。