Job ID = 6458067 SRX = SRX495197 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:22:04 prefetch.2.10.7: 1) Downloading 'SRR1198702'... 2020-06-21T12:22:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:24:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:24:43 prefetch.2.10.7: 1) 'SRR1198702' was downloaded successfully Read 21457757 spots for SRR1198702/SRR1198702.sra Written 21457757 spots for SRR1198702/SRR1198702.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:36 21457757 reads; of these: 21457757 (100.00%) were unpaired; of these: 542456 (2.53%) aligned 0 times 16529295 (77.03%) aligned exactly 1 time 4386006 (20.44%) aligned >1 times 97.47% overall alignment rate Time searching: 00:05:36 Overall time: 00:05:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4812663 / 20915301 = 0.2301 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:16: 1000000 INFO @ Sun, 21 Jun 2020 21:36:21: 2000000 INFO @ Sun, 21 Jun 2020 21:36:26: 3000000 INFO @ Sun, 21 Jun 2020 21:36:32: 4000000 INFO @ Sun, 21 Jun 2020 21:36:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:43: 6000000 INFO @ Sun, 21 Jun 2020 21:36:46: 1000000 INFO @ Sun, 21 Jun 2020 21:36:48: 7000000 INFO @ Sun, 21 Jun 2020 21:36:52: 2000000 INFO @ Sun, 21 Jun 2020 21:36:54: 8000000 INFO @ Sun, 21 Jun 2020 21:36:58: 3000000 INFO @ Sun, 21 Jun 2020 21:37:00: 9000000 INFO @ Sun, 21 Jun 2020 21:37:03: 4000000 INFO @ Sun, 21 Jun 2020 21:37:05: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:37:09: 5000000 INFO @ Sun, 21 Jun 2020 21:37:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:37:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:37:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:37:11: 11000000 INFO @ Sun, 21 Jun 2020 21:37:15: 6000000 INFO @ Sun, 21 Jun 2020 21:37:16: 1000000 INFO @ Sun, 21 Jun 2020 21:37:17: 12000000 INFO @ Sun, 21 Jun 2020 21:37:21: 7000000 INFO @ Sun, 21 Jun 2020 21:37:22: 2000000 INFO @ Sun, 21 Jun 2020 21:37:22: 13000000 INFO @ Sun, 21 Jun 2020 21:37:26: 8000000 INFO @ Sun, 21 Jun 2020 21:37:28: 3000000 INFO @ Sun, 21 Jun 2020 21:37:28: 14000000 INFO @ Sun, 21 Jun 2020 21:37:32: 9000000 INFO @ Sun, 21 Jun 2020 21:37:33: 4000000 INFO @ Sun, 21 Jun 2020 21:37:34: 15000000 INFO @ Sun, 21 Jun 2020 21:37:38: 10000000 INFO @ Sun, 21 Jun 2020 21:37:39: 5000000 INFO @ Sun, 21 Jun 2020 21:37:40: 16000000 INFO @ Sun, 21 Jun 2020 21:37:41: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:37:41: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:37:41: #1 total tags in treatment: 16102638 INFO @ Sun, 21 Jun 2020 21:37:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:41: #1 tags after filtering in treatment: 16102637 INFO @ Sun, 21 Jun 2020 21:37:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:42: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:37:42: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:43: 11000000 INFO @ Sun, 21 Jun 2020 21:37:45: 6000000 INFO @ Sun, 21 Jun 2020 21:37:49: 12000000 INFO @ Sun, 21 Jun 2020 21:37:50: 7000000 INFO @ Sun, 21 Jun 2020 21:37:54: 13000000 INFO @ Sun, 21 Jun 2020 21:37:56: 8000000 INFO @ Sun, 21 Jun 2020 21:38:01: 14000000 INFO @ Sun, 21 Jun 2020 21:38:02: 9000000 INFO @ Sun, 21 Jun 2020 21:38:06: 15000000 INFO @ Sun, 21 Jun 2020 21:38:07: 10000000 INFO @ Sun, 21 Jun 2020 21:38:12: 16000000 INFO @ Sun, 21 Jun 2020 21:38:13: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:38:13: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:38:13: #1 total tags in treatment: 16102638 INFO @ Sun, 21 Jun 2020 21:38:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:38:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:38:13: 11000000 INFO @ Sun, 21 Jun 2020 21:38:13: #1 tags after filtering in treatment: 16102637 INFO @ Sun, 21 Jun 2020 21:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:38:13: #1 finished! INFO @ Sun, 21 Jun 2020 21:38:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:38:15: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:38:15: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:38:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:38:18: 12000000 INFO @ Sun, 21 Jun 2020 21:38:23: 13000000 INFO @ Sun, 21 Jun 2020 21:38:29: 14000000 INFO @ Sun, 21 Jun 2020 21:38:34: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:38:40: 16000000 INFO @ Sun, 21 Jun 2020 21:38:40: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:38:40: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:38:40: #1 total tags in treatment: 16102638 INFO @ Sun, 21 Jun 2020 21:38:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:38:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:38:41: #1 tags after filtering in treatment: 16102637 INFO @ Sun, 21 Jun 2020 21:38:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:38:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:38:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:38:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:38:42: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:38:42: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:38:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495197/SRX495197.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。