Job ID = 6458066 SRX = SRX495196 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:06:36 prefetch.2.10.7: 1) Downloading 'SRR1198701'... 2020-06-21T12:06:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:09:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:09:09 prefetch.2.10.7: 1) 'SRR1198701' was downloaded successfully Read 23287832 spots for SRR1198701/SRR1198701.sra Written 23287832 spots for SRR1198701/SRR1198701.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 23287832 reads; of these: 23287832 (100.00%) were unpaired; of these: 2705814 (11.62%) aligned 0 times 18066589 (77.58%) aligned exactly 1 time 2515429 (10.80%) aligned >1 times 88.38% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4316945 / 20582018 = 0.2097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:20:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:20:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:20:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:20:05: 1000000 INFO @ Sun, 21 Jun 2020 21:20:10: 2000000 INFO @ Sun, 21 Jun 2020 21:20:15: 3000000 INFO @ Sun, 21 Jun 2020 21:20:20: 4000000 INFO @ Sun, 21 Jun 2020 21:20:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:20:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:20:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:20:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:20:30: 6000000 INFO @ Sun, 21 Jun 2020 21:20:35: 1000000 INFO @ Sun, 21 Jun 2020 21:20:35: 7000000 INFO @ Sun, 21 Jun 2020 21:20:40: 2000000 INFO @ Sun, 21 Jun 2020 21:20:41: 8000000 INFO @ Sun, 21 Jun 2020 21:20:46: 3000000 INFO @ Sun, 21 Jun 2020 21:20:46: 9000000 INFO @ Sun, 21 Jun 2020 21:20:51: 4000000 INFO @ Sun, 21 Jun 2020 21:20:51: 10000000 INFO @ Sun, 21 Jun 2020 21:20:56: 5000000 INFO @ Sun, 21 Jun 2020 21:20:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:21:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:21:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:21:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:21:01: 6000000 INFO @ Sun, 21 Jun 2020 21:21:02: 12000000 INFO @ Sun, 21 Jun 2020 21:21:05: 1000000 INFO @ Sun, 21 Jun 2020 21:21:07: 7000000 INFO @ Sun, 21 Jun 2020 21:21:07: 13000000 INFO @ Sun, 21 Jun 2020 21:21:11: 2000000 INFO @ Sun, 21 Jun 2020 21:21:12: 8000000 INFO @ Sun, 21 Jun 2020 21:21:12: 14000000 INFO @ Sun, 21 Jun 2020 21:21:16: 3000000 INFO @ Sun, 21 Jun 2020 21:21:17: 9000000 INFO @ Sun, 21 Jun 2020 21:21:18: 15000000 INFO @ Sun, 21 Jun 2020 21:21:21: 4000000 INFO @ Sun, 21 Jun 2020 21:21:22: 10000000 INFO @ Sun, 21 Jun 2020 21:21:23: 16000000 INFO @ Sun, 21 Jun 2020 21:21:25: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:21:25: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:21:25: #1 total tags in treatment: 16265073 INFO @ Sun, 21 Jun 2020 21:21:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:21:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:21:25: #1 tags after filtering in treatment: 16264972 INFO @ Sun, 21 Jun 2020 21:21:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:21:25: #1 finished! INFO @ Sun, 21 Jun 2020 21:21:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:21:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:21:26: 5000000 INFO @ Sun, 21 Jun 2020 21:21:26: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 21:21:26: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:21:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:21:28: 11000000 INFO @ Sun, 21 Jun 2020 21:21:31: 6000000 INFO @ Sun, 21 Jun 2020 21:21:33: 12000000 INFO @ Sun, 21 Jun 2020 21:21:36: 7000000 INFO @ Sun, 21 Jun 2020 21:21:38: 13000000 INFO @ Sun, 21 Jun 2020 21:21:42: 8000000 INFO @ Sun, 21 Jun 2020 21:21:43: 14000000 INFO @ Sun, 21 Jun 2020 21:21:47: 9000000 INFO @ Sun, 21 Jun 2020 21:21:49: 15000000 INFO @ Sun, 21 Jun 2020 21:21:52: 10000000 INFO @ Sun, 21 Jun 2020 21:21:54: 16000000 INFO @ Sun, 21 Jun 2020 21:21:55: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:21:55: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:21:55: #1 total tags in treatment: 16265073 INFO @ Sun, 21 Jun 2020 21:21:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:21:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:21:56: #1 tags after filtering in treatment: 16264972 INFO @ Sun, 21 Jun 2020 21:21:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:21:56: #1 finished! INFO @ Sun, 21 Jun 2020 21:21:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:21:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:21:57: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 21:21:57: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:21:57: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 21:21:57: 11000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:22:02: 12000000 INFO @ Sun, 21 Jun 2020 21:22:07: 13000000 INFO @ Sun, 21 Jun 2020 21:22:13: 14000000 INFO @ Sun, 21 Jun 2020 21:22:17: 15000000 INFO @ Sun, 21 Jun 2020 21:22:22: 16000000 INFO @ Sun, 21 Jun 2020 21:22:24: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:22:24: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:22:24: #1 total tags in treatment: 16265073 INFO @ Sun, 21 Jun 2020 21:22:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:22:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:22:24: #1 tags after filtering in treatment: 16264972 INFO @ Sun, 21 Jun 2020 21:22:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:22:24: #1 finished! INFO @ Sun, 21 Jun 2020 21:22:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:22:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:22:26: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 21:22:26: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:22:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495196/SRX495196.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。