Job ID = 6508825 SRX = SRX495194 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:23:21 prefetch.2.10.7: 1) Downloading 'SRR1198699'... 2020-06-26T15:23:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:25:33 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:25:33 prefetch.2.10.7: 1) 'SRR1198699' was downloaded successfully Read 21914988 spots for SRR1198699/SRR1198699.sra Written 21914988 spots for SRR1198699/SRR1198699.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:56 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2170288 (9.90%) aligned 0 times 17713649 (80.83%) aligned exactly 1 time 2031051 (9.27%) aligned >1 times 90.10% overall alignment rate Time searching: 00:03:57 Overall time: 00:03:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3723044 / 19744700 = 0.1886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:36:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:36:29: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:36:29: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:36:35: 1000000 INFO @ Sat, 27 Jun 2020 00:36:41: 2000000 INFO @ Sat, 27 Jun 2020 00:36:47: 3000000 INFO @ Sat, 27 Jun 2020 00:36:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:36:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:36:58: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:36:58: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:37:00: 5000000 INFO @ Sat, 27 Jun 2020 00:37:05: 1000000 INFO @ Sat, 27 Jun 2020 00:37:06: 6000000 INFO @ Sat, 27 Jun 2020 00:37:11: 2000000 INFO @ Sat, 27 Jun 2020 00:37:12: 7000000 INFO @ Sat, 27 Jun 2020 00:37:17: 3000000 INFO @ Sat, 27 Jun 2020 00:37:18: 8000000 INFO @ Sat, 27 Jun 2020 00:37:23: 4000000 INFO @ Sat, 27 Jun 2020 00:37:25: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:37:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:37:29: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:37:29: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:37:29: 5000000 INFO @ Sat, 27 Jun 2020 00:37:31: 10000000 INFO @ Sat, 27 Jun 2020 00:37:35: 1000000 INFO @ Sat, 27 Jun 2020 00:37:35: 6000000 INFO @ Sat, 27 Jun 2020 00:37:37: 11000000 INFO @ Sat, 27 Jun 2020 00:37:41: 2000000 INFO @ Sat, 27 Jun 2020 00:37:42: 7000000 INFO @ Sat, 27 Jun 2020 00:37:43: 12000000 INFO @ Sat, 27 Jun 2020 00:37:47: 3000000 INFO @ Sat, 27 Jun 2020 00:37:48: 8000000 INFO @ Sat, 27 Jun 2020 00:37:50: 13000000 INFO @ Sat, 27 Jun 2020 00:37:53: 4000000 INFO @ Sat, 27 Jun 2020 00:37:54: 9000000 INFO @ Sat, 27 Jun 2020 00:37:56: 14000000 INFO @ Sat, 27 Jun 2020 00:37:59: 5000000 INFO @ Sat, 27 Jun 2020 00:38:00: 10000000 INFO @ Sat, 27 Jun 2020 00:38:03: 15000000 INFO @ Sat, 27 Jun 2020 00:38:05: 6000000 INFO @ Sat, 27 Jun 2020 00:38:06: 11000000 INFO @ Sat, 27 Jun 2020 00:38:09: 16000000 INFO @ Sat, 27 Jun 2020 00:38:09: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:38:09: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:38:09: #1 total tags in treatment: 16021656 INFO @ Sat, 27 Jun 2020 00:38:09: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:38:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:38:10: #1 tags after filtering in treatment: 16021570 INFO @ Sat, 27 Jun 2020 00:38:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:38:10: #1 finished! INFO @ Sat, 27 Jun 2020 00:38:10: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:38:11: 7000000 INFO @ Sat, 27 Jun 2020 00:38:11: #2 number of paired peaks: 23 WARNING @ Sat, 27 Jun 2020 00:38:11: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:38:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:38:12: 12000000 INFO @ Sat, 27 Jun 2020 00:38:16: 8000000 INFO @ Sat, 27 Jun 2020 00:38:18: 13000000 INFO @ Sat, 27 Jun 2020 00:38:22: 9000000 INFO @ Sat, 27 Jun 2020 00:38:24: 14000000 INFO @ Sat, 27 Jun 2020 00:38:27: 10000000 INFO @ Sat, 27 Jun 2020 00:38:29: 15000000 INFO @ Sat, 27 Jun 2020 00:38:33: 11000000 INFO @ Sat, 27 Jun 2020 00:38:35: 16000000 INFO @ Sat, 27 Jun 2020 00:38:36: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:38:36: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:38:36: #1 total tags in treatment: 16021656 INFO @ Sat, 27 Jun 2020 00:38:36: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:38:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:38:36: #1 tags after filtering in treatment: 16021570 INFO @ Sat, 27 Jun 2020 00:38:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:38:36: #1 finished! INFO @ Sat, 27 Jun 2020 00:38:36: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:38:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:38:37: #2 number of paired peaks: 23 WARNING @ Sat, 27 Jun 2020 00:38:37: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:38:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:38:38: 12000000 INFO @ Sat, 27 Jun 2020 00:38:43: 13000000 INFO @ Sat, 27 Jun 2020 00:38:48: 14000000 INFO @ Sat, 27 Jun 2020 00:38:53: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:38:59: 16000000 INFO @ Sat, 27 Jun 2020 00:38:59: #1 tag size is determined as 44 bps INFO @ Sat, 27 Jun 2020 00:38:59: #1 tag size = 44 INFO @ Sat, 27 Jun 2020 00:38:59: #1 total tags in treatment: 16021656 INFO @ Sat, 27 Jun 2020 00:38:59: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:39:00: #1 tags after filtering in treatment: 16021570 INFO @ Sat, 27 Jun 2020 00:39:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:39:00: #1 finished! INFO @ Sat, 27 Jun 2020 00:39:00: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:39:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:39:01: #2 number of paired peaks: 23 WARNING @ Sat, 27 Jun 2020 00:39:01: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:39:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495194/SRX495194.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。