Job ID = 6458063 SRX = SRX495193 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:06:06 prefetch.2.10.7: 1) Downloading 'SRR1198698'... 2020-06-21T12:06:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:09:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:09:00 prefetch.2.10.7: 1) 'SRR1198698' was downloaded successfully Read 21914988 spots for SRR1198698/SRR1198698.sra Written 21914988 spots for SRR1198698/SRR1198698.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:00 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2170291 (9.90%) aligned 0 times 17713631 (80.83%) aligned exactly 1 time 2031066 (9.27%) aligned >1 times 90.10% overall alignment rate Time searching: 00:04:00 Overall time: 00:04:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3723822 / 19744697 = 0.1886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:18:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:18:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:18:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:18:39: 1000000 INFO @ Sun, 21 Jun 2020 21:18:43: 2000000 INFO @ Sun, 21 Jun 2020 21:18:48: 3000000 INFO @ Sun, 21 Jun 2020 21:18:53: 4000000 INFO @ Sun, 21 Jun 2020 21:18:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:19:03: 6000000 INFO @ Sun, 21 Jun 2020 21:19:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:19:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:19:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:19:08: 7000000 INFO @ Sun, 21 Jun 2020 21:19:10: 1000000 INFO @ Sun, 21 Jun 2020 21:19:13: 8000000 INFO @ Sun, 21 Jun 2020 21:19:16: 2000000 INFO @ Sun, 21 Jun 2020 21:19:18: 9000000 INFO @ Sun, 21 Jun 2020 21:19:22: 3000000 INFO @ Sun, 21 Jun 2020 21:19:23: 10000000 INFO @ Sun, 21 Jun 2020 21:19:28: 11000000 INFO @ Sun, 21 Jun 2020 21:19:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:19:34: 12000000 INFO @ Sun, 21 Jun 2020 21:19:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:19:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:19:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:19:35: 5000000 INFO @ Sun, 21 Jun 2020 21:19:39: 1000000 INFO @ Sun, 21 Jun 2020 21:19:39: 13000000 INFO @ Sun, 21 Jun 2020 21:19:41: 6000000 INFO @ Sun, 21 Jun 2020 21:19:45: 14000000 INFO @ Sun, 21 Jun 2020 21:19:45: 2000000 INFO @ Sun, 21 Jun 2020 21:19:47: 7000000 INFO @ Sun, 21 Jun 2020 21:19:50: 15000000 INFO @ Sun, 21 Jun 2020 21:19:50: 3000000 INFO @ Sun, 21 Jun 2020 21:19:53: 8000000 INFO @ Sun, 21 Jun 2020 21:19:55: 4000000 INFO @ Sun, 21 Jun 2020 21:19:56: 16000000 INFO @ Sun, 21 Jun 2020 21:19:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:19:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:19:56: #1 total tags in treatment: 16020875 INFO @ Sun, 21 Jun 2020 21:19:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:19:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:19:56: #1 tags after filtering in treatment: 16020797 INFO @ Sun, 21 Jun 2020 21:19:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:19:56: #1 finished! INFO @ Sun, 21 Jun 2020 21:19:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:19:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:19:57: #2 number of paired peaks: 24 WARNING @ Sun, 21 Jun 2020 21:19:57: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:19:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:19:59: 9000000 INFO @ Sun, 21 Jun 2020 21:20:01: 5000000 INFO @ Sun, 21 Jun 2020 21:20:05: 10000000 INFO @ Sun, 21 Jun 2020 21:20:06: 6000000 INFO @ Sun, 21 Jun 2020 21:20:10: 11000000 INFO @ Sun, 21 Jun 2020 21:20:11: 7000000 INFO @ Sun, 21 Jun 2020 21:20:16: 12000000 INFO @ Sun, 21 Jun 2020 21:20:17: 8000000 INFO @ Sun, 21 Jun 2020 21:20:22: 9000000 INFO @ Sun, 21 Jun 2020 21:20:23: 13000000 INFO @ Sun, 21 Jun 2020 21:20:27: 10000000 INFO @ Sun, 21 Jun 2020 21:20:28: 14000000 INFO @ Sun, 21 Jun 2020 21:20:32: 11000000 INFO @ Sun, 21 Jun 2020 21:20:34: 15000000 INFO @ Sun, 21 Jun 2020 21:20:37: 12000000 INFO @ Sun, 21 Jun 2020 21:20:40: 16000000 INFO @ Sun, 21 Jun 2020 21:20:40: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:20:40: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:20:40: #1 total tags in treatment: 16020875 INFO @ Sun, 21 Jun 2020 21:20:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:41: #1 tags after filtering in treatment: 16020797 INFO @ Sun, 21 Jun 2020 21:20:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:42: #2 number of paired peaks: 24 WARNING @ Sun, 21 Jun 2020 21:20:42: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:20:43: 13000000 INFO @ Sun, 21 Jun 2020 21:20:48: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:20:52: 15000000 INFO @ Sun, 21 Jun 2020 21:20:57: 16000000 INFO @ Sun, 21 Jun 2020 21:20:58: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:20:58: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:20:58: #1 total tags in treatment: 16020875 INFO @ Sun, 21 Jun 2020 21:20:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:58: #1 tags after filtering in treatment: 16020797 INFO @ Sun, 21 Jun 2020 21:20:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:58: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:59: #2 number of paired peaks: 24 WARNING @ Sun, 21 Jun 2020 21:20:59: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495193/SRX495193.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。