Job ID = 6458062 SRX = SRX495192 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:11:34 prefetch.2.10.7: 1) Downloading 'SRR1198697'... 2020-06-21T12:11:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:14:06 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:14:07 prefetch.2.10.7: 'SRR1198697' is valid 2020-06-21T12:14:07 prefetch.2.10.7: 1) 'SRR1198697' was downloaded successfully Read 18534845 spots for SRR1198697/SRR1198697.sra Written 18534845 spots for SRR1198697/SRR1198697.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:37 18534845 reads; of these: 18534845 (100.00%) were unpaired; of these: 704765 (3.80%) aligned 0 times 15730034 (84.87%) aligned exactly 1 time 2100046 (11.33%) aligned >1 times 96.20% overall alignment rate Time searching: 00:03:37 Overall time: 00:03:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3965150 / 17830080 = 0.2224 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:22:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:22:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:22:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:22:50: 1000000 INFO @ Sun, 21 Jun 2020 21:22:55: 2000000 INFO @ Sun, 21 Jun 2020 21:23:01: 3000000 INFO @ Sun, 21 Jun 2020 21:23:06: 4000000 INFO @ Sun, 21 Jun 2020 21:23:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:18: 6000000 INFO @ Sun, 21 Jun 2020 21:23:21: 1000000 INFO @ Sun, 21 Jun 2020 21:23:25: 7000000 INFO @ Sun, 21 Jun 2020 21:23:29: 2000000 INFO @ Sun, 21 Jun 2020 21:23:31: 8000000 INFO @ Sun, 21 Jun 2020 21:23:36: 3000000 INFO @ Sun, 21 Jun 2020 21:23:38: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:43: 4000000 INFO @ Sun, 21 Jun 2020 21:23:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:44: 10000000 INFO @ Sun, 21 Jun 2020 21:23:50: 5000000 INFO @ Sun, 21 Jun 2020 21:23:51: 11000000 INFO @ Sun, 21 Jun 2020 21:23:51: 1000000 INFO @ Sun, 21 Jun 2020 21:23:57: 6000000 INFO @ Sun, 21 Jun 2020 21:23:58: 2000000 INFO @ Sun, 21 Jun 2020 21:23:58: 12000000 INFO @ Sun, 21 Jun 2020 21:24:04: 7000000 INFO @ Sun, 21 Jun 2020 21:24:04: 3000000 INFO @ Sun, 21 Jun 2020 21:24:05: 13000000 INFO @ Sun, 21 Jun 2020 21:24:11: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:24:11: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:24:11: #1 total tags in treatment: 13864930 INFO @ Sun, 21 Jun 2020 21:24:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:24:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:24:11: 8000000 INFO @ Sun, 21 Jun 2020 21:24:11: 4000000 INFO @ Sun, 21 Jun 2020 21:24:11: #1 tags after filtering in treatment: 13864918 INFO @ Sun, 21 Jun 2020 21:24:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:24:11: #1 finished! INFO @ Sun, 21 Jun 2020 21:24:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:24:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:24:12: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 21:24:12: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:24:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:24:18: 5000000 INFO @ Sun, 21 Jun 2020 21:24:18: 9000000 INFO @ Sun, 21 Jun 2020 21:24:25: 6000000 INFO @ Sun, 21 Jun 2020 21:24:25: 10000000 INFO @ Sun, 21 Jun 2020 21:24:31: 7000000 INFO @ Sun, 21 Jun 2020 21:24:33: 11000000 INFO @ Sun, 21 Jun 2020 21:24:38: 8000000 INFO @ Sun, 21 Jun 2020 21:24:40: 12000000 INFO @ Sun, 21 Jun 2020 21:24:45: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:24:47: 13000000 INFO @ Sun, 21 Jun 2020 21:24:51: 10000000 INFO @ Sun, 21 Jun 2020 21:24:54: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:24:54: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:24:54: #1 total tags in treatment: 13864930 INFO @ Sun, 21 Jun 2020 21:24:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:24:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:24:54: #1 tags after filtering in treatment: 13864918 INFO @ Sun, 21 Jun 2020 21:24:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:24:54: #1 finished! INFO @ Sun, 21 Jun 2020 21:24:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:24:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:24:55: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 21:24:55: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:24:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:24:58: 11000000 INFO @ Sun, 21 Jun 2020 21:25:04: 12000000 INFO @ Sun, 21 Jun 2020 21:25:09: 13000000 INFO @ Sun, 21 Jun 2020 21:25:14: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:25:14: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:25:14: #1 total tags in treatment: 13864930 INFO @ Sun, 21 Jun 2020 21:25:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:15: #1 tags after filtering in treatment: 13864918 INFO @ Sun, 21 Jun 2020 21:25:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:16: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 21:25:16: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495192/SRX495192.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。