Job ID = 6458060 SRX = SRX495190 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:01:47 prefetch.2.10.7: 1) Downloading 'SRR1198695'... 2020-06-21T12:01:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:03:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:03:45 prefetch.2.10.7: 'SRR1198695' is valid 2020-06-21T12:03:45 prefetch.2.10.7: 1) 'SRR1198695' was downloaded successfully Read 16205479 spots for SRR1198695/SRR1198695.sra Written 16205479 spots for SRR1198695/SRR1198695.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:19 16205479 reads; of these: 16205479 (100.00%) were unpaired; of these: 192458 (1.19%) aligned 0 times 13436965 (82.92%) aligned exactly 1 time 2576056 (15.90%) aligned >1 times 98.81% overall alignment rate Time searching: 00:03:19 Overall time: 00:03:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2957812 / 16013021 = 0.1847 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:11:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:11:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:11:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:11:47: 1000000 INFO @ Sun, 21 Jun 2020 21:11:52: 2000000 INFO @ Sun, 21 Jun 2020 21:11:58: 3000000 INFO @ Sun, 21 Jun 2020 21:12:03: 4000000 INFO @ Sun, 21 Jun 2020 21:12:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:12:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:12:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:12:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:12:13: 6000000 INFO @ Sun, 21 Jun 2020 21:12:18: 1000000 INFO @ Sun, 21 Jun 2020 21:12:19: 7000000 INFO @ Sun, 21 Jun 2020 21:12:24: 2000000 INFO @ Sun, 21 Jun 2020 21:12:25: 8000000 INFO @ Sun, 21 Jun 2020 21:12:30: 3000000 INFO @ Sun, 21 Jun 2020 21:12:31: 9000000 INFO @ Sun, 21 Jun 2020 21:12:36: 4000000 INFO @ Sun, 21 Jun 2020 21:12:37: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:12:42: 5000000 INFO @ Sun, 21 Jun 2020 21:12:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:12:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:12:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:12:43: 11000000 INFO @ Sun, 21 Jun 2020 21:12:48: 6000000 INFO @ Sun, 21 Jun 2020 21:12:48: 1000000 INFO @ Sun, 21 Jun 2020 21:12:49: 12000000 INFO @ Sun, 21 Jun 2020 21:12:54: 2000000 INFO @ Sun, 21 Jun 2020 21:12:54: 7000000 INFO @ Sun, 21 Jun 2020 21:12:55: 13000000 INFO @ Sun, 21 Jun 2020 21:12:55: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:12:55: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:12:55: #1 total tags in treatment: 13055209 INFO @ Sun, 21 Jun 2020 21:12:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:12:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:12:56: #1 tags after filtering in treatment: 13055202 INFO @ Sun, 21 Jun 2020 21:12:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:12:56: #1 finished! INFO @ Sun, 21 Jun 2020 21:12:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:12:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:12:57: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:12:57: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:12:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:13:00: 3000000 INFO @ Sun, 21 Jun 2020 21:13:00: 8000000 INFO @ Sun, 21 Jun 2020 21:13:05: 4000000 INFO @ Sun, 21 Jun 2020 21:13:06: 9000000 INFO @ Sun, 21 Jun 2020 21:13:11: 5000000 INFO @ Sun, 21 Jun 2020 21:13:12: 10000000 INFO @ Sun, 21 Jun 2020 21:13:17: 6000000 INFO @ Sun, 21 Jun 2020 21:13:18: 11000000 INFO @ Sun, 21 Jun 2020 21:13:23: 7000000 INFO @ Sun, 21 Jun 2020 21:13:24: 12000000 INFO @ Sun, 21 Jun 2020 21:13:28: 8000000 INFO @ Sun, 21 Jun 2020 21:13:30: 13000000 INFO @ Sun, 21 Jun 2020 21:13:30: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:13:30: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:13:30: #1 total tags in treatment: 13055209 INFO @ Sun, 21 Jun 2020 21:13:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:13:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:13:31: #1 tags after filtering in treatment: 13055202 INFO @ Sun, 21 Jun 2020 21:13:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:13:31: #1 finished! INFO @ Sun, 21 Jun 2020 21:13:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:13:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:13:31: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:13:31: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:13:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:13:34: 9000000 INFO @ Sun, 21 Jun 2020 21:13:39: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:13:45: 11000000 INFO @ Sun, 21 Jun 2020 21:13:50: 12000000 INFO @ Sun, 21 Jun 2020 21:13:55: 13000000 INFO @ Sun, 21 Jun 2020 21:13:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:13:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:13:56: #1 total tags in treatment: 13055209 INFO @ Sun, 21 Jun 2020 21:13:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:13:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:13:56: #1 tags after filtering in treatment: 13055202 INFO @ Sun, 21 Jun 2020 21:13:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:13:56: #1 finished! INFO @ Sun, 21 Jun 2020 21:13:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:13:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:13:57: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:13:57: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:13:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495190/SRX495190.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。