Job ID = 6458059 SRX = SRX495189 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:25:20 prefetch.2.10.7: 1) Downloading 'SRR1198694'... 2020-06-21T12:25:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:27:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:27:15 prefetch.2.10.7: 'SRR1198694' is valid 2020-06-21T12:27:15 prefetch.2.10.7: 1) 'SRR1198694' was downloaded successfully Read 16205479 spots for SRR1198694/SRR1198694.sra Written 16205479 spots for SRR1198694/SRR1198694.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:30 16205479 reads; of these: 16205479 (100.00%) were unpaired; of these: 192409 (1.19%) aligned 0 times 13436971 (82.92%) aligned exactly 1 time 2576099 (15.90%) aligned >1 times 98.81% overall alignment rate Time searching: 00:03:30 Overall time: 00:03:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2958288 / 16013070 = 0.1847 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:39: 1000000 INFO @ Sun, 21 Jun 2020 21:35:44: 2000000 INFO @ Sun, 21 Jun 2020 21:35:49: 3000000 INFO @ Sun, 21 Jun 2020 21:35:55: 4000000 INFO @ Sun, 21 Jun 2020 21:36:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:06: 6000000 INFO @ Sun, 21 Jun 2020 21:36:11: 1000000 INFO @ Sun, 21 Jun 2020 21:36:12: 7000000 INFO @ Sun, 21 Jun 2020 21:36:18: 2000000 INFO @ Sun, 21 Jun 2020 21:36:18: 8000000 INFO @ Sun, 21 Jun 2020 21:36:25: 3000000 INFO @ Sun, 21 Jun 2020 21:36:25: 9000000 INFO @ Sun, 21 Jun 2020 21:36:31: 10000000 INFO @ Sun, 21 Jun 2020 21:36:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:38: 11000000 INFO @ Sun, 21 Jun 2020 21:36:39: 5000000 INFO @ Sun, 21 Jun 2020 21:36:41: 1000000 INFO @ Sun, 21 Jun 2020 21:36:44: 12000000 INFO @ Sun, 21 Jun 2020 21:36:46: 6000000 INFO @ Sun, 21 Jun 2020 21:36:48: 2000000 INFO @ Sun, 21 Jun 2020 21:36:51: 13000000 INFO @ Sun, 21 Jun 2020 21:36:51: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:36:51: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:36:51: #1 total tags in treatment: 13054782 INFO @ Sun, 21 Jun 2020 21:36:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:52: #1 tags after filtering in treatment: 13054778 INFO @ Sun, 21 Jun 2020 21:36:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:52: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 21:36:52: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05_peaks.narrowPeak: No such file or directory INFO @ Sun, 21 Jun 2020 21:36:53: 7000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:36:55: 3000000 INFO @ Sun, 21 Jun 2020 21:37:00: 8000000 INFO @ Sun, 21 Jun 2020 21:37:02: 4000000 INFO @ Sun, 21 Jun 2020 21:37:08: 9000000 INFO @ Sun, 21 Jun 2020 21:37:09: 5000000 INFO @ Sun, 21 Jun 2020 21:37:15: 10000000 INFO @ Sun, 21 Jun 2020 21:37:16: 6000000 INFO @ Sun, 21 Jun 2020 21:37:22: 11000000 INFO @ Sun, 21 Jun 2020 21:37:23: 7000000 INFO @ Sun, 21 Jun 2020 21:37:29: 12000000 INFO @ Sun, 21 Jun 2020 21:37:30: 8000000 INFO @ Sun, 21 Jun 2020 21:37:36: 13000000 INFO @ Sun, 21 Jun 2020 21:37:37: 9000000 INFO @ Sun, 21 Jun 2020 21:37:37: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:37:37: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:37:37: #1 total tags in treatment: 13054782 INFO @ Sun, 21 Jun 2020 21:37:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:37: #1 tags after filtering in treatment: 13054778 INFO @ Sun, 21 Jun 2020 21:37:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:37: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:38: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 21:37:38: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:43: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:37:50: 11000000 INFO @ Sun, 21 Jun 2020 21:37:57: 12000000 INFO @ Sun, 21 Jun 2020 21:38:03: 13000000 INFO @ Sun, 21 Jun 2020 21:38:04: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:38:04: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:38:04: #1 total tags in treatment: 13054782 INFO @ Sun, 21 Jun 2020 21:38:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:38:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:38:04: #1 tags after filtering in treatment: 13054778 INFO @ Sun, 21 Jun 2020 21:38:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:38:04: #1 finished! INFO @ Sun, 21 Jun 2020 21:38:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:38:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:38:05: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 21:38:05: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:38:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495189/SRX495189.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。