Job ID = 6458052 SRX = SRX495183 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:12:19 prefetch.2.10.7: 1) Downloading 'SRR1198688'... 2020-06-21T12:12:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:13:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:13:56 prefetch.2.10.7: 1) 'SRR1198688' was downloaded successfully Read 17982328 spots for SRR1198688/SRR1198688.sra Written 17982328 spots for SRR1198688/SRR1198688.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:53 17982328 reads; of these: 17982328 (100.00%) were unpaired; of these: 998616 (5.55%) aligned 0 times 14237285 (79.17%) aligned exactly 1 time 2746427 (15.27%) aligned >1 times 94.45% overall alignment rate Time searching: 00:03:53 Overall time: 00:03:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3433077 / 16983712 = 0.2021 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:22:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:22:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:22:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:05: 1000000 INFO @ Sun, 21 Jun 2020 21:23:12: 2000000 INFO @ Sun, 21 Jun 2020 21:23:18: 3000000 INFO @ Sun, 21 Jun 2020 21:23:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:30: 5000000 INFO @ Sun, 21 Jun 2020 21:23:36: 1000000 INFO @ Sun, 21 Jun 2020 21:23:37: 6000000 INFO @ Sun, 21 Jun 2020 21:23:42: 2000000 INFO @ Sun, 21 Jun 2020 21:23:44: 7000000 INFO @ Sun, 21 Jun 2020 21:23:48: 3000000 INFO @ Sun, 21 Jun 2020 21:23:50: 8000000 INFO @ Sun, 21 Jun 2020 21:23:55: 4000000 INFO @ Sun, 21 Jun 2020 21:23:57: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:01: 5000000 INFO @ Sun, 21 Jun 2020 21:24:04: 10000000 INFO @ Sun, 21 Jun 2020 21:24:06: 1000000 INFO @ Sun, 21 Jun 2020 21:24:08: 6000000 INFO @ Sun, 21 Jun 2020 21:24:11: 11000000 INFO @ Sun, 21 Jun 2020 21:24:13: 2000000 INFO @ Sun, 21 Jun 2020 21:24:14: 7000000 INFO @ Sun, 21 Jun 2020 21:24:18: 12000000 INFO @ Sun, 21 Jun 2020 21:24:20: 3000000 INFO @ Sun, 21 Jun 2020 21:24:21: 8000000 INFO @ Sun, 21 Jun 2020 21:24:25: 13000000 INFO @ Sun, 21 Jun 2020 21:24:26: 4000000 INFO @ Sun, 21 Jun 2020 21:24:27: 9000000 INFO @ Sun, 21 Jun 2020 21:24:29: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:24:29: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:24:29: #1 total tags in treatment: 13550635 INFO @ Sun, 21 Jun 2020 21:24:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:24:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:24:29: #1 tags after filtering in treatment: 13550631 INFO @ Sun, 21 Jun 2020 21:24:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:24:29: #1 finished! INFO @ Sun, 21 Jun 2020 21:24:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:24:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:24:30: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 21:24:30: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:24:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:24:33: 5000000 INFO @ Sun, 21 Jun 2020 21:24:33: 10000000 INFO @ Sun, 21 Jun 2020 21:24:39: 6000000 INFO @ Sun, 21 Jun 2020 21:24:40: 11000000 INFO @ Sun, 21 Jun 2020 21:24:45: 7000000 INFO @ Sun, 21 Jun 2020 21:24:46: 12000000 INFO @ Sun, 21 Jun 2020 21:24:52: 8000000 INFO @ Sun, 21 Jun 2020 21:24:53: 13000000 INFO @ Sun, 21 Jun 2020 21:24:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:24:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:24:56: #1 total tags in treatment: 13550635 INFO @ Sun, 21 Jun 2020 21:24:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:24:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:24:57: #1 tags after filtering in treatment: 13550631 INFO @ Sun, 21 Jun 2020 21:24:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:24:57: #1 finished! INFO @ Sun, 21 Jun 2020 21:24:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:24:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:24:58: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 21:24:58: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:24:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:24:58: 9000000 INFO @ Sun, 21 Jun 2020 21:25:05: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:25:11: 11000000 INFO @ Sun, 21 Jun 2020 21:25:17: 12000000 INFO @ Sun, 21 Jun 2020 21:25:22: 13000000 INFO @ Sun, 21 Jun 2020 21:25:26: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:25:26: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:25:26: #1 total tags in treatment: 13550635 INFO @ Sun, 21 Jun 2020 21:25:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:27: #1 tags after filtering in treatment: 13550631 INFO @ Sun, 21 Jun 2020 21:25:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:28: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 21:25:28: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495183/SRX495183.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。