Job ID = 6458048 SRX = SRX495179 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:22:04 prefetch.2.10.7: 1) Downloading 'SRR1198684'... 2020-06-21T12:22:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:24:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:24:55 prefetch.2.10.7: 1) 'SRR1198684' was downloaded successfully Read 19728174 spots for SRR1198684/SRR1198684.sra Written 19728174 spots for SRR1198684/SRR1198684.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:56 19728174 reads; of these: 19728174 (100.00%) were unpaired; of these: 3361524 (17.04%) aligned 0 times 13525143 (68.56%) aligned exactly 1 time 2841507 (14.40%) aligned >1 times 82.96% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3569054 / 16366650 = 0.2181 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:34:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:34:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:34:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:01: 1000000 INFO @ Sun, 21 Jun 2020 21:35:08: 2000000 INFO @ Sun, 21 Jun 2020 21:35:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:22: 4000000 INFO @ Sun, 21 Jun 2020 21:35:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:29: 5000000 INFO @ Sun, 21 Jun 2020 21:35:32: 1000000 INFO @ Sun, 21 Jun 2020 21:35:36: 6000000 INFO @ Sun, 21 Jun 2020 21:35:40: 2000000 INFO @ Sun, 21 Jun 2020 21:35:44: 7000000 INFO @ Sun, 21 Jun 2020 21:35:48: 3000000 INFO @ Sun, 21 Jun 2020 21:35:51: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:56: 4000000 INFO @ Sun, 21 Jun 2020 21:35:58: 9000000 INFO @ Sun, 21 Jun 2020 21:36:02: 1000000 INFO @ Sun, 21 Jun 2020 21:36:05: 5000000 INFO @ Sun, 21 Jun 2020 21:36:05: 10000000 INFO @ Sun, 21 Jun 2020 21:36:11: 2000000 INFO @ Sun, 21 Jun 2020 21:36:14: 11000000 INFO @ Sun, 21 Jun 2020 21:36:14: 6000000 INFO @ Sun, 21 Jun 2020 21:36:20: 3000000 INFO @ Sun, 21 Jun 2020 21:36:21: 12000000 INFO @ Sun, 21 Jun 2020 21:36:22: 7000000 INFO @ Sun, 21 Jun 2020 21:36:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:36:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:36:27: #1 total tags in treatment: 12797596 INFO @ Sun, 21 Jun 2020 21:36:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:28: #1 tags after filtering in treatment: 12797592 INFO @ Sun, 21 Jun 2020 21:36:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:29: 4000000 INFO @ Sun, 21 Jun 2020 21:36:29: #2 number of paired peaks: 49 WARNING @ Sun, 21 Jun 2020 21:36:29: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:36:31: 8000000 INFO @ Sun, 21 Jun 2020 21:36:37: 5000000 INFO @ Sun, 21 Jun 2020 21:36:39: 9000000 INFO @ Sun, 21 Jun 2020 21:36:45: 6000000 INFO @ Sun, 21 Jun 2020 21:36:47: 10000000 INFO @ Sun, 21 Jun 2020 21:36:53: 7000000 INFO @ Sun, 21 Jun 2020 21:36:56: 11000000 INFO @ Sun, 21 Jun 2020 21:37:02: 8000000 INFO @ Sun, 21 Jun 2020 21:37:04: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:37:10: 9000000 INFO @ Sun, 21 Jun 2020 21:37:11: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:37:11: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:37:11: #1 total tags in treatment: 12797596 INFO @ Sun, 21 Jun 2020 21:37:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:12: #1 tags after filtering in treatment: 12797592 INFO @ Sun, 21 Jun 2020 21:37:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:12: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:13: #2 number of paired peaks: 49 WARNING @ Sun, 21 Jun 2020 21:37:13: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:17: 10000000 INFO @ Sun, 21 Jun 2020 21:37:26: 11000000 INFO @ Sun, 21 Jun 2020 21:37:34: 12000000 INFO @ Sun, 21 Jun 2020 21:37:40: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:37:40: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:37:40: #1 total tags in treatment: 12797596 INFO @ Sun, 21 Jun 2020 21:37:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:41: #1 tags after filtering in treatment: 12797592 INFO @ Sun, 21 Jun 2020 21:37:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:42: #2 number of paired peaks: 49 WARNING @ Sun, 21 Jun 2020 21:37:42: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495179/SRX495179.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。