Job ID = 6458043 SRX = SRX495177 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:16:34 prefetch.2.10.7: 1) Downloading 'SRR1198682'... 2020-06-21T12:16:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:19:03 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:19:03 prefetch.2.10.7: 1) 'SRR1198682' was downloaded successfully Read 18970395 spots for SRR1198682/SRR1198682.sra Written 18970395 spots for SRR1198682/SRR1198682.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:16 18970395 reads; of these: 18970395 (100.00%) were unpaired; of these: 359207 (1.89%) aligned 0 times 15141519 (79.82%) aligned exactly 1 time 3469669 (18.29%) aligned >1 times 98.11% overall alignment rate Time searching: 00:04:16 Overall time: 00:04:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4049464 / 18611188 = 0.2176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:28:35: 1000000 INFO @ Sun, 21 Jun 2020 21:28:40: 2000000 INFO @ Sun, 21 Jun 2020 21:28:46: 3000000 INFO @ Sun, 21 Jun 2020 21:28:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:57: 5000000 INFO @ Sun, 21 Jun 2020 21:28:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:29:03: 6000000 INFO @ Sun, 21 Jun 2020 21:29:05: 1000000 INFO @ Sun, 21 Jun 2020 21:29:09: 7000000 INFO @ Sun, 21 Jun 2020 21:29:12: 2000000 INFO @ Sun, 21 Jun 2020 21:29:16: 8000000 INFO @ Sun, 21 Jun 2020 21:29:18: 3000000 INFO @ Sun, 21 Jun 2020 21:29:22: 9000000 INFO @ Sun, 21 Jun 2020 21:29:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:29:28: 10000000 INFO @ Sun, 21 Jun 2020 21:29:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:29:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:29:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:29:31: 5000000 INFO @ Sun, 21 Jun 2020 21:29:35: 11000000 INFO @ Sun, 21 Jun 2020 21:29:37: 1000000 INFO @ Sun, 21 Jun 2020 21:29:38: 6000000 INFO @ Sun, 21 Jun 2020 21:29:42: 12000000 INFO @ Sun, 21 Jun 2020 21:29:44: 2000000 INFO @ Sun, 21 Jun 2020 21:29:45: 7000000 INFO @ Sun, 21 Jun 2020 21:29:49: 13000000 INFO @ Sun, 21 Jun 2020 21:29:51: 3000000 INFO @ Sun, 21 Jun 2020 21:29:52: 8000000 INFO @ Sun, 21 Jun 2020 21:29:56: 14000000 INFO @ Sun, 21 Jun 2020 21:29:59: 4000000 INFO @ Sun, 21 Jun 2020 21:29:59: 9000000 INFO @ Sun, 21 Jun 2020 21:30:00: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:30:00: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:30:00: #1 total tags in treatment: 14561724 INFO @ Sun, 21 Jun 2020 21:30:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:30:00: #1 tags after filtering in treatment: 14561722 INFO @ Sun, 21 Jun 2020 21:30:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:30:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:30:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:30:01: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:30:01: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:30:06: 10000000 INFO @ Sun, 21 Jun 2020 21:30:06: 5000000 INFO @ Sun, 21 Jun 2020 21:30:12: 11000000 INFO @ Sun, 21 Jun 2020 21:30:13: 6000000 INFO @ Sun, 21 Jun 2020 21:30:19: 12000000 INFO @ Sun, 21 Jun 2020 21:30:21: 7000000 INFO @ Sun, 21 Jun 2020 21:30:25: 13000000 INFO @ Sun, 21 Jun 2020 21:30:28: 8000000 INFO @ Sun, 21 Jun 2020 21:30:32: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:30:35: 9000000 INFO @ Sun, 21 Jun 2020 21:30:36: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:30:36: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:30:36: #1 total tags in treatment: 14561724 INFO @ Sun, 21 Jun 2020 21:30:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:30:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:30:36: #1 tags after filtering in treatment: 14561722 INFO @ Sun, 21 Jun 2020 21:30:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:30:36: #1 finished! INFO @ Sun, 21 Jun 2020 21:30:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:30:37: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:30:37: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:30:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:30:42: 10000000 INFO @ Sun, 21 Jun 2020 21:30:48: 11000000 INFO @ Sun, 21 Jun 2020 21:30:55: 12000000 INFO @ Sun, 21 Jun 2020 21:31:02: 13000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:31:09: 14000000 INFO @ Sun, 21 Jun 2020 21:31:13: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:31:13: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:31:13: #1 total tags in treatment: 14561724 INFO @ Sun, 21 Jun 2020 21:31:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:31:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:31:13: #1 tags after filtering in treatment: 14561722 INFO @ Sun, 21 Jun 2020 21:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:31:13: #1 finished! INFO @ Sun, 21 Jun 2020 21:31:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:31:14: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 21:31:14: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:31:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495177/SRX495177.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling