Job ID = 6458042 SRX = SRX495176 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:19:04 prefetch.2.10.7: 1) Downloading 'SRR1198681'... 2020-06-21T12:19:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:56 prefetch.2.10.7: 1) 'SRR1198681' was downloaded successfully Read 22157841 spots for SRR1198681/SRR1198681.sra Written 22157841 spots for SRR1198681/SRR1198681.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:03 22157841 reads; of these: 22157841 (100.00%) were unpaired; of these: 1867069 (8.43%) aligned 0 times 15987063 (72.15%) aligned exactly 1 time 4303709 (19.42%) aligned >1 times 91.57% overall alignment rate Time searching: 00:05:03 Overall time: 00:05:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5082663 / 20290772 = 0.2505 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:34:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:34:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:34:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:34:19: 1000000 INFO @ Sun, 21 Jun 2020 21:34:24: 2000000 INFO @ Sun, 21 Jun 2020 21:34:29: 3000000 INFO @ Sun, 21 Jun 2020 21:34:35: 4000000 INFO @ Sun, 21 Jun 2020 21:34:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:34:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:34:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:34:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:34:46: 6000000 INFO @ Sun, 21 Jun 2020 21:34:49: 1000000 INFO @ Sun, 21 Jun 2020 21:34:52: 7000000 INFO @ Sun, 21 Jun 2020 21:34:54: 2000000 INFO @ Sun, 21 Jun 2020 21:34:58: 8000000 INFO @ Sun, 21 Jun 2020 21:34:59: 3000000 INFO @ Sun, 21 Jun 2020 21:35:04: 9000000 INFO @ Sun, 21 Jun 2020 21:35:04: 4000000 INFO @ Sun, 21 Jun 2020 21:35:10: 10000000 INFO @ Sun, 21 Jun 2020 21:35:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:15: 11000000 INFO @ Sun, 21 Jun 2020 21:35:16: 6000000 INFO @ Sun, 21 Jun 2020 21:35:19: 1000000 INFO @ Sun, 21 Jun 2020 21:35:21: 12000000 INFO @ Sun, 21 Jun 2020 21:35:22: 7000000 INFO @ Sun, 21 Jun 2020 21:35:25: 2000000 INFO @ Sun, 21 Jun 2020 21:35:28: 8000000 INFO @ Sun, 21 Jun 2020 21:35:28: 13000000 INFO @ Sun, 21 Jun 2020 21:35:31: 3000000 INFO @ Sun, 21 Jun 2020 21:35:33: 9000000 INFO @ Sun, 21 Jun 2020 21:35:33: 14000000 INFO @ Sun, 21 Jun 2020 21:35:37: 4000000 INFO @ Sun, 21 Jun 2020 21:35:39: 10000000 INFO @ Sun, 21 Jun 2020 21:35:40: 15000000 INFO @ Sun, 21 Jun 2020 21:35:41: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:35:41: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:35:41: #1 total tags in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:35:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:42: #1 tags after filtering in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:35:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:42: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:42: 5000000 INFO @ Sun, 21 Jun 2020 21:35:43: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 21:35:43: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:35:44: 11000000 INFO @ Sun, 21 Jun 2020 21:35:47: 6000000 INFO @ Sun, 21 Jun 2020 21:35:49: 12000000 INFO @ Sun, 21 Jun 2020 21:35:53: 7000000 INFO @ Sun, 21 Jun 2020 21:35:56: 13000000 INFO @ Sun, 21 Jun 2020 21:35:58: 8000000 INFO @ Sun, 21 Jun 2020 21:36:01: 14000000 INFO @ Sun, 21 Jun 2020 21:36:03: 9000000 INFO @ Sun, 21 Jun 2020 21:36:06: 15000000 INFO @ Sun, 21 Jun 2020 21:36:08: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:36:08: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:36:08: #1 total tags in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:36:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:08: 10000000 INFO @ Sun, 21 Jun 2020 21:36:09: #1 tags after filtering in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:36:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:09: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:09: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:10: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 21:36:10: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:36:13: 11000000 INFO @ Sun, 21 Jun 2020 21:36:18: 12000000 INFO @ Sun, 21 Jun 2020 21:36:24: 13000000 INFO @ Sun, 21 Jun 2020 21:36:30: 14000000 INFO @ Sun, 21 Jun 2020 21:36:35: 15000000 INFO @ Sun, 21 Jun 2020 21:36:37: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:36:37: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:36:37: #1 total tags in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:36:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:37: #1 tags after filtering in treatment: 15208109 INFO @ Sun, 21 Jun 2020 21:36:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:37: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:38: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 21:36:38: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495176/SRX495176.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。