Job ID = 6458040 SRX = SRX495174 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:14:20 prefetch.2.10.7: 1) Downloading 'SRR1198679'... 2020-06-21T12:14:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:48 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:48 prefetch.2.10.7: 1) 'SRR1198679' was downloaded successfully Read 21802283 spots for SRR1198679/SRR1198679.sra Written 21802283 spots for SRR1198679/SRR1198679.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:06 21802283 reads; of these: 21802283 (100.00%) were unpaired; of these: 597252 (2.74%) aligned 0 times 17456818 (80.07%) aligned exactly 1 time 3748213 (17.19%) aligned >1 times 97.26% overall alignment rate Time searching: 00:05:06 Overall time: 00:05:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4815644 / 21205031 = 0.2271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:21: 1000000 INFO @ Sun, 21 Jun 2020 21:27:26: 2000000 INFO @ Sun, 21 Jun 2020 21:27:31: 3000000 INFO @ Sun, 21 Jun 2020 21:27:37: 4000000 INFO @ Sun, 21 Jun 2020 21:27:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:48: 6000000 INFO @ Sun, 21 Jun 2020 21:27:51: 1000000 INFO @ Sun, 21 Jun 2020 21:27:54: 7000000 INFO @ Sun, 21 Jun 2020 21:27:57: 2000000 INFO @ Sun, 21 Jun 2020 21:28:00: 8000000 INFO @ Sun, 21 Jun 2020 21:28:04: 3000000 INFO @ Sun, 21 Jun 2020 21:28:06: 9000000 INFO @ Sun, 21 Jun 2020 21:28:10: 4000000 INFO @ Sun, 21 Jun 2020 21:28:12: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:28:16: 5000000 INFO @ Sun, 21 Jun 2020 21:28:18: 11000000 INFO @ Sun, 21 Jun 2020 21:28:21: 1000000 INFO @ Sun, 21 Jun 2020 21:28:22: 6000000 INFO @ Sun, 21 Jun 2020 21:28:25: 12000000 INFO @ Sun, 21 Jun 2020 21:28:27: 2000000 INFO @ Sun, 21 Jun 2020 21:28:28: 7000000 INFO @ Sun, 21 Jun 2020 21:28:31: 13000000 INFO @ Sun, 21 Jun 2020 21:28:34: 3000000 INFO @ Sun, 21 Jun 2020 21:28:34: 8000000 INFO @ Sun, 21 Jun 2020 21:28:37: 14000000 INFO @ Sun, 21 Jun 2020 21:28:40: 4000000 INFO @ Sun, 21 Jun 2020 21:28:41: 9000000 INFO @ Sun, 21 Jun 2020 21:28:44: 15000000 INFO @ Sun, 21 Jun 2020 21:28:46: 5000000 INFO @ Sun, 21 Jun 2020 21:28:47: 10000000 INFO @ Sun, 21 Jun 2020 21:28:50: 16000000 INFO @ Sun, 21 Jun 2020 21:28:52: 6000000 INFO @ Sun, 21 Jun 2020 21:28:53: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:28:53: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:28:53: #1 total tags in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:28:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:28:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:28:53: 11000000 INFO @ Sun, 21 Jun 2020 21:28:53: #1 tags after filtering in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:28:53: #1 finished! INFO @ Sun, 21 Jun 2020 21:28:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:28:54: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:28:54: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:28:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:28:58: 7000000 INFO @ Sun, 21 Jun 2020 21:28:59: 12000000 INFO @ Sun, 21 Jun 2020 21:29:04: 8000000 INFO @ Sun, 21 Jun 2020 21:29:05: 13000000 INFO @ Sun, 21 Jun 2020 21:29:10: 9000000 INFO @ Sun, 21 Jun 2020 21:29:12: 14000000 INFO @ Sun, 21 Jun 2020 21:29:16: 10000000 INFO @ Sun, 21 Jun 2020 21:29:18: 15000000 INFO @ Sun, 21 Jun 2020 21:29:22: 11000000 INFO @ Sun, 21 Jun 2020 21:29:24: 16000000 INFO @ Sun, 21 Jun 2020 21:29:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:29:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:29:27: #1 total tags in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:29:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:28: #1 tags after filtering in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:29:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:29: 12000000 INFO @ Sun, 21 Jun 2020 21:29:29: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:29:29: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:29:34: 13000000 INFO @ Sun, 21 Jun 2020 21:29:40: 14000000 INFO @ Sun, 21 Jun 2020 21:29:46: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:29:52: 16000000 INFO @ Sun, 21 Jun 2020 21:29:54: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:29:54: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:29:54: #1 total tags in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:29:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:55: #1 tags after filtering in treatment: 16389387 INFO @ Sun, 21 Jun 2020 21:29:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:55: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:56: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:29:56: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495174/SRX495174.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。