Job ID = 6458039 SRX = SRX495173 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:19:04 prefetch.2.10.7: 1) Downloading 'SRR1198678'... 2020-06-21T12:19:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:10 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:10 prefetch.2.10.7: 1) 'SRR1198678' was downloaded successfully Read 21802283 spots for SRR1198678/SRR1198678.sra Written 21802283 spots for SRR1198678/SRR1198678.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:10 21802283 reads; of these: 21802283 (100.00%) were unpaired; of these: 597260 (2.74%) aligned 0 times 17456855 (80.07%) aligned exactly 1 time 3748168 (17.19%) aligned >1 times 97.26% overall alignment rate Time searching: 00:05:10 Overall time: 00:05:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4816199 / 21205023 = 0.2271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:30: 1000000 INFO @ Sun, 21 Jun 2020 21:32:35: 2000000 INFO @ Sun, 21 Jun 2020 21:32:40: 3000000 INFO @ Sun, 21 Jun 2020 21:32:46: 4000000 INFO @ Sun, 21 Jun 2020 21:32:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:57: 6000000 INFO @ Sun, 21 Jun 2020 21:33:00: 1000000 INFO @ Sun, 21 Jun 2020 21:33:03: 7000000 INFO @ Sun, 21 Jun 2020 21:33:06: 2000000 INFO @ Sun, 21 Jun 2020 21:33:09: 8000000 INFO @ Sun, 21 Jun 2020 21:33:13: 3000000 INFO @ Sun, 21 Jun 2020 21:33:15: 9000000 INFO @ Sun, 21 Jun 2020 21:33:19: 4000000 INFO @ Sun, 21 Jun 2020 21:33:21: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:25: 5000000 INFO @ Sun, 21 Jun 2020 21:33:28: 11000000 INFO @ Sun, 21 Jun 2020 21:33:30: 1000000 INFO @ Sun, 21 Jun 2020 21:33:31: 6000000 INFO @ Sun, 21 Jun 2020 21:33:34: 12000000 INFO @ Sun, 21 Jun 2020 21:33:37: 2000000 INFO @ Sun, 21 Jun 2020 21:33:37: 7000000 INFO @ Sun, 21 Jun 2020 21:33:40: 13000000 INFO @ Sun, 21 Jun 2020 21:33:43: 3000000 INFO @ Sun, 21 Jun 2020 21:33:43: 8000000 INFO @ Sun, 21 Jun 2020 21:33:46: 14000000 INFO @ Sun, 21 Jun 2020 21:33:49: 4000000 INFO @ Sun, 21 Jun 2020 21:33:49: 9000000 INFO @ Sun, 21 Jun 2020 21:33:52: 15000000 INFO @ Sun, 21 Jun 2020 21:33:55: 5000000 INFO @ Sun, 21 Jun 2020 21:33:55: 10000000 INFO @ Sun, 21 Jun 2020 21:33:58: 16000000 INFO @ Sun, 21 Jun 2020 21:34:01: 6000000 INFO @ Sun, 21 Jun 2020 21:34:01: 11000000 INFO @ Sun, 21 Jun 2020 21:34:01: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:34:01: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:34:01: #1 total tags in treatment: 16388824 INFO @ Sun, 21 Jun 2020 21:34:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:34:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:34:02: #1 tags after filtering in treatment: 16388823 INFO @ Sun, 21 Jun 2020 21:34:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:34:02: #1 finished! INFO @ Sun, 21 Jun 2020 21:34:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:34:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:34:03: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:34:03: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:34:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:34:07: 7000000 INFO @ Sun, 21 Jun 2020 21:34:07: 12000000 INFO @ Sun, 21 Jun 2020 21:34:13: 8000000 INFO @ Sun, 21 Jun 2020 21:34:13: 13000000 INFO @ Sun, 21 Jun 2020 21:34:19: 9000000 INFO @ Sun, 21 Jun 2020 21:34:20: 14000000 INFO @ Sun, 21 Jun 2020 21:34:25: 10000000 INFO @ Sun, 21 Jun 2020 21:34:26: 15000000 INFO @ Sun, 21 Jun 2020 21:34:31: 11000000 INFO @ Sun, 21 Jun 2020 21:34:32: 16000000 INFO @ Sun, 21 Jun 2020 21:34:35: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:34:35: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:34:35: #1 total tags in treatment: 16388824 INFO @ Sun, 21 Jun 2020 21:34:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:34:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:34:35: #1 tags after filtering in treatment: 16388823 INFO @ Sun, 21 Jun 2020 21:34:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:34:35: #1 finished! INFO @ Sun, 21 Jun 2020 21:34:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:34:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:34:36: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:34:36: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:34:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:34:37: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:34:43: 13000000 INFO @ Sun, 21 Jun 2020 21:34:49: 14000000 INFO @ Sun, 21 Jun 2020 21:34:55: 15000000 INFO @ Sun, 21 Jun 2020 21:35:00: 16000000 INFO @ Sun, 21 Jun 2020 21:35:03: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:35:03: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:35:03: #1 total tags in treatment: 16388824 INFO @ Sun, 21 Jun 2020 21:35:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:03: #1 tags after filtering in treatment: 16388823 INFO @ Sun, 21 Jun 2020 21:35:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:03: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:04: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:35:04: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495173/SRX495173.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。