Job ID = 6458037 SRX = SRX495171 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:01:32 prefetch.2.10.7: 1) Downloading 'SRR1198676'... 2020-06-21T12:01:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:05:40 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:05:40 prefetch.2.10.7: 1) 'SRR1198676' was downloaded successfully Read 27747945 spots for SRR1198676/SRR1198676.sra Written 27747945 spots for SRR1198676/SRR1198676.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 27747945 reads; of these: 27747945 (100.00%) were unpaired; of these: 5426115 (19.56%) aligned 0 times 18297941 (65.94%) aligned exactly 1 time 4023889 (14.50%) aligned >1 times 80.44% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6642442 / 22321830 = 0.2976 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:23: 1000000 INFO @ Sun, 21 Jun 2020 21:17:30: 2000000 INFO @ Sun, 21 Jun 2020 21:17:37: 3000000 INFO @ Sun, 21 Jun 2020 21:17:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:52: 5000000 INFO @ Sun, 21 Jun 2020 21:17:54: 1000000 INFO @ Sun, 21 Jun 2020 21:17:59: 6000000 INFO @ Sun, 21 Jun 2020 21:18:01: 2000000 INFO @ Sun, 21 Jun 2020 21:18:07: 7000000 INFO @ Sun, 21 Jun 2020 21:18:09: 3000000 INFO @ Sun, 21 Jun 2020 21:18:14: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:18:16: 4000000 INFO @ Sun, 21 Jun 2020 21:18:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:18:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:18:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:18:22: 9000000 INFO @ Sun, 21 Jun 2020 21:18:23: 5000000 INFO @ Sun, 21 Jun 2020 21:18:24: 1000000 INFO @ Sun, 21 Jun 2020 21:18:30: 10000000 INFO @ Sun, 21 Jun 2020 21:18:31: 6000000 INFO @ Sun, 21 Jun 2020 21:18:32: 2000000 INFO @ Sun, 21 Jun 2020 21:18:38: 11000000 INFO @ Sun, 21 Jun 2020 21:18:38: 7000000 INFO @ Sun, 21 Jun 2020 21:18:40: 3000000 INFO @ Sun, 21 Jun 2020 21:18:45: 12000000 INFO @ Sun, 21 Jun 2020 21:18:45: 8000000 INFO @ Sun, 21 Jun 2020 21:18:48: 4000000 INFO @ Sun, 21 Jun 2020 21:18:53: 9000000 INFO @ Sun, 21 Jun 2020 21:18:53: 13000000 INFO @ Sun, 21 Jun 2020 21:18:55: 5000000 INFO @ Sun, 21 Jun 2020 21:19:00: 10000000 INFO @ Sun, 21 Jun 2020 21:19:01: 14000000 INFO @ Sun, 21 Jun 2020 21:19:03: 6000000 INFO @ Sun, 21 Jun 2020 21:19:08: 11000000 INFO @ Sun, 21 Jun 2020 21:19:09: 15000000 INFO @ Sun, 21 Jun 2020 21:19:11: 7000000 INFO @ Sun, 21 Jun 2020 21:19:14: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:19:14: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:19:14: #1 total tags in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:19:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:19:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:19:15: #1 tags after filtering in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:19:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:19:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:19:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:19:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:19:15: 12000000 INFO @ Sun, 21 Jun 2020 21:19:16: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:19:16: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:19:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:19:18: 8000000 INFO @ Sun, 21 Jun 2020 21:19:23: 13000000 INFO @ Sun, 21 Jun 2020 21:19:26: 9000000 INFO @ Sun, 21 Jun 2020 21:19:30: 14000000 INFO @ Sun, 21 Jun 2020 21:19:34: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:19:37: 15000000 INFO @ Sun, 21 Jun 2020 21:19:41: 11000000 INFO @ Sun, 21 Jun 2020 21:19:43: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:19:43: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:19:43: #1 total tags in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:19:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:19:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:19:43: #1 tags after filtering in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:19:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:19:43: #1 finished! INFO @ Sun, 21 Jun 2020 21:19:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:19:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:19:44: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:19:44: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:19:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:19:48: 12000000 INFO @ Sun, 21 Jun 2020 21:19:56: 13000000 INFO @ Sun, 21 Jun 2020 21:20:03: 14000000 INFO @ Sun, 21 Jun 2020 21:20:09: 15000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:20:14: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:20:14: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:20:14: #1 total tags in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:20:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:15: #1 tags after filtering in treatment: 15679388 INFO @ Sun, 21 Jun 2020 21:20:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:16: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:20:16: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495171/SRX495171.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling