Job ID = 6458036 SRX = SRX495170 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:09:51 prefetch.2.10.7: 1) Downloading 'SRR1198675'... 2020-06-21T12:09:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:12:29 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:12:29 prefetch.2.10.7: 1) 'SRR1198675' was downloaded successfully Read 21802283 spots for SRR1198675/SRR1198675.sra Written 21802283 spots for SRR1198675/SRR1198675.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 21802283 reads; of these: 21802283 (100.00%) were unpaired; of these: 597244 (2.74%) aligned 0 times 17456859 (80.07%) aligned exactly 1 time 3748180 (17.19%) aligned >1 times 97.26% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4816200 / 21205039 = 0.2271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:56: 1000000 INFO @ Sun, 21 Jun 2020 21:24:01: 2000000 INFO @ Sun, 21 Jun 2020 21:24:07: 3000000 INFO @ Sun, 21 Jun 2020 21:24:12: 4000000 INFO @ Sun, 21 Jun 2020 21:24:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:24:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:24:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:24:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:23: 6000000 INFO @ Sun, 21 Jun 2020 21:24:26: 1000000 INFO @ Sun, 21 Jun 2020 21:24:29: 7000000 INFO @ Sun, 21 Jun 2020 21:24:32: 2000000 INFO @ Sun, 21 Jun 2020 21:24:35: 8000000 INFO @ Sun, 21 Jun 2020 21:24:38: 3000000 INFO @ Sun, 21 Jun 2020 21:24:41: 9000000 INFO @ Sun, 21 Jun 2020 21:24:45: 4000000 INFO @ Sun, 21 Jun 2020 21:24:47: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:24:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:24:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:24:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:50: 5000000 INFO @ Sun, 21 Jun 2020 21:24:53: 11000000 INFO @ Sun, 21 Jun 2020 21:24:56: 1000000 INFO @ Sun, 21 Jun 2020 21:24:57: 6000000 INFO @ Sun, 21 Jun 2020 21:24:59: 12000000 INFO @ Sun, 21 Jun 2020 21:25:03: 2000000 INFO @ Sun, 21 Jun 2020 21:25:03: 7000000 INFO @ Sun, 21 Jun 2020 21:25:05: 13000000 INFO @ Sun, 21 Jun 2020 21:25:09: 8000000 INFO @ Sun, 21 Jun 2020 21:25:09: 3000000 INFO @ Sun, 21 Jun 2020 21:25:12: 14000000 INFO @ Sun, 21 Jun 2020 21:25:15: 9000000 INFO @ Sun, 21 Jun 2020 21:25:15: 4000000 INFO @ Sun, 21 Jun 2020 21:25:18: 15000000 INFO @ Sun, 21 Jun 2020 21:25:21: 10000000 INFO @ Sun, 21 Jun 2020 21:25:21: 5000000 INFO @ Sun, 21 Jun 2020 21:25:24: 16000000 INFO @ Sun, 21 Jun 2020 21:25:26: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:25:26: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:25:26: #1 total tags in treatment: 16388839 INFO @ Sun, 21 Jun 2020 21:25:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:27: 11000000 INFO @ Sun, 21 Jun 2020 21:25:27: 6000000 INFO @ Sun, 21 Jun 2020 21:25:27: #1 tags after filtering in treatment: 16388837 INFO @ Sun, 21 Jun 2020 21:25:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:28: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:25:28: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:25:33: 7000000 INFO @ Sun, 21 Jun 2020 21:25:33: 12000000 INFO @ Sun, 21 Jun 2020 21:25:39: 8000000 INFO @ Sun, 21 Jun 2020 21:25:39: 13000000 INFO @ Sun, 21 Jun 2020 21:25:45: 9000000 INFO @ Sun, 21 Jun 2020 21:25:45: 14000000 INFO @ Sun, 21 Jun 2020 21:25:51: 10000000 INFO @ Sun, 21 Jun 2020 21:25:51: 15000000 INFO @ Sun, 21 Jun 2020 21:25:57: 11000000 INFO @ Sun, 21 Jun 2020 21:25:57: 16000000 INFO @ Sun, 21 Jun 2020 21:26:00: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:26:00: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:26:00: #1 total tags in treatment: 16388839 INFO @ Sun, 21 Jun 2020 21:26:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:00: #1 tags after filtering in treatment: 16388837 INFO @ Sun, 21 Jun 2020 21:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:01: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:26:01: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:26:03: 12000000 INFO @ Sun, 21 Jun 2020 21:26:08: 13000000 INFO @ Sun, 21 Jun 2020 21:26:14: 14000000 INFO @ Sun, 21 Jun 2020 21:26:19: 15000000 INFO @ Sun, 21 Jun 2020 21:26:25: 16000000 INFO @ Sun, 21 Jun 2020 21:26:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:26:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:26:27: #1 total tags in treatment: 16388839 INFO @ Sun, 21 Jun 2020 21:26:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:27: #1 tags after filtering in treatment: 16388837 INFO @ Sun, 21 Jun 2020 21:26:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:29: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:26:29: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495170/SRX495170.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。