Job ID = 6458032 SRX = SRX495166 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:12:34 prefetch.2.10.7: 1) Downloading 'SRR1198671'... 2020-06-21T12:12:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:32 prefetch.2.10.7: 'SRR1198671' is valid 2020-06-21T12:15:32 prefetch.2.10.7: 1) 'SRR1198671' was downloaded successfully Read 19927623 spots for SRR1198671/SRR1198671.sra Written 19927623 spots for SRR1198671/SRR1198671.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:19 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 336301 (1.69%) aligned 0 times 15093155 (75.74%) aligned exactly 1 time 4498167 (22.57%) aligned >1 times 98.31% overall alignment rate Time searching: 00:05:19 Overall time: 00:05:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4748133 / 19591322 = 0.2424 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:40: 1000000 INFO @ Sun, 21 Jun 2020 21:26:46: 2000000 INFO @ Sun, 21 Jun 2020 21:26:51: 3000000 INFO @ Sun, 21 Jun 2020 21:26:56: 4000000 INFO @ Sun, 21 Jun 2020 21:27:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:08: 6000000 INFO @ Sun, 21 Jun 2020 21:27:12: 1000000 INFO @ Sun, 21 Jun 2020 21:27:14: 7000000 INFO @ Sun, 21 Jun 2020 21:27:18: 2000000 INFO @ Sun, 21 Jun 2020 21:27:20: 8000000 INFO @ Sun, 21 Jun 2020 21:27:24: 3000000 INFO @ Sun, 21 Jun 2020 21:27:26: 9000000 INFO @ Sun, 21 Jun 2020 21:27:30: 4000000 INFO @ Sun, 21 Jun 2020 21:27:33: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:37: 5000000 INFO @ Sun, 21 Jun 2020 21:27:39: 11000000 INFO @ Sun, 21 Jun 2020 21:27:42: 1000000 INFO @ Sun, 21 Jun 2020 21:27:43: 6000000 INFO @ Sun, 21 Jun 2020 21:27:45: 12000000 INFO @ Sun, 21 Jun 2020 21:27:49: 2000000 INFO @ Sun, 21 Jun 2020 21:27:50: 7000000 INFO @ Sun, 21 Jun 2020 21:27:53: 13000000 INFO @ Sun, 21 Jun 2020 21:27:56: 3000000 INFO @ Sun, 21 Jun 2020 21:27:56: 8000000 INFO @ Sun, 21 Jun 2020 21:27:59: 14000000 INFO @ Sun, 21 Jun 2020 21:28:03: 4000000 INFO @ Sun, 21 Jun 2020 21:28:03: 9000000 INFO @ Sun, 21 Jun 2020 21:28:05: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:28:05: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:28:05: #1 total tags in treatment: 14843189 INFO @ Sun, 21 Jun 2020 21:28:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:28:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:28:06: #1 tags after filtering in treatment: 14843188 INFO @ Sun, 21 Jun 2020 21:28:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:28:06: #1 finished! INFO @ Sun, 21 Jun 2020 21:28:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:28:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:28:07: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:28:07: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:28:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:28:10: 10000000 INFO @ Sun, 21 Jun 2020 21:28:10: 5000000 INFO @ Sun, 21 Jun 2020 21:28:16: 11000000 INFO @ Sun, 21 Jun 2020 21:28:17: 6000000 INFO @ Sun, 21 Jun 2020 21:28:22: 12000000 INFO @ Sun, 21 Jun 2020 21:28:24: 7000000 INFO @ Sun, 21 Jun 2020 21:28:29: 13000000 INFO @ Sun, 21 Jun 2020 21:28:30: 8000000 INFO @ Sun, 21 Jun 2020 21:28:35: 14000000 INFO @ Sun, 21 Jun 2020 21:28:36: 9000000 INFO @ Sun, 21 Jun 2020 21:28:41: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:28:41: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:28:41: #1 total tags in treatment: 14843189 INFO @ Sun, 21 Jun 2020 21:28:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:28:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:28:41: #1 tags after filtering in treatment: 14843188 INFO @ Sun, 21 Jun 2020 21:28:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:28:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:28:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:28:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:28:42: 10000000 INFO @ Sun, 21 Jun 2020 21:28:42: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:28:42: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:28:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:28:48: 11000000 INFO @ Sun, 21 Jun 2020 21:28:54: 12000000 INFO @ Sun, 21 Jun 2020 21:29:00: 13000000 INFO @ Sun, 21 Jun 2020 21:29:06: 14000000 INFO @ Sun, 21 Jun 2020 21:29:12: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:29:12: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:29:12: #1 total tags in treatment: 14843189 INFO @ Sun, 21 Jun 2020 21:29:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:12: #1 tags after filtering in treatment: 14843188 INFO @ Sun, 21 Jun 2020 21:29:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:12: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:13: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:29:13: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495166/SRX495166.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。