Job ID = 6458031 SRX = SRX495165 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:04:04 prefetch.2.10.7: 1) Downloading 'SRR1198670'... 2020-06-21T12:04:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:06:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:06:29 prefetch.2.10.7: 'SRR1198670' is valid 2020-06-21T12:06:29 prefetch.2.10.7: 1) 'SRR1198670' was downloaded successfully Read 19927623 spots for SRR1198670/SRR1198670.sra Written 19927623 spots for SRR1198670/SRR1198670.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:54 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 336487 (1.69%) aligned 0 times 15093037 (75.74%) aligned exactly 1 time 4498099 (22.57%) aligned >1 times 98.31% overall alignment rate Time searching: 00:04:54 Overall time: 00:04:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4748244 / 19591136 = 0.2424 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:55: 1000000 INFO @ Sun, 21 Jun 2020 21:17:01: 2000000 INFO @ Sun, 21 Jun 2020 21:17:07: 3000000 INFO @ Sun, 21 Jun 2020 21:17:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:20: 5000000 INFO @ Sun, 21 Jun 2020 21:17:25: 1000000 INFO @ Sun, 21 Jun 2020 21:17:27: 6000000 INFO @ Sun, 21 Jun 2020 21:17:31: 2000000 INFO @ Sun, 21 Jun 2020 21:17:33: 7000000 INFO @ Sun, 21 Jun 2020 21:17:38: 3000000 INFO @ Sun, 21 Jun 2020 21:17:40: 8000000 INFO @ Sun, 21 Jun 2020 21:17:44: 4000000 INFO @ Sun, 21 Jun 2020 21:17:47: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:50: 5000000 INFO @ Sun, 21 Jun 2020 21:17:53: 10000000 INFO @ Sun, 21 Jun 2020 21:17:55: 1000000 INFO @ Sun, 21 Jun 2020 21:17:56: 6000000 INFO @ Sun, 21 Jun 2020 21:18:00: 11000000 INFO @ Sun, 21 Jun 2020 21:18:01: 2000000 INFO @ Sun, 21 Jun 2020 21:18:02: 7000000 INFO @ Sun, 21 Jun 2020 21:18:07: 12000000 INFO @ Sun, 21 Jun 2020 21:18:08: 3000000 INFO @ Sun, 21 Jun 2020 21:18:08: 8000000 INFO @ Sun, 21 Jun 2020 21:18:14: 4000000 INFO @ Sun, 21 Jun 2020 21:18:14: 13000000 INFO @ Sun, 21 Jun 2020 21:18:14: 9000000 INFO @ Sun, 21 Jun 2020 21:18:20: 5000000 INFO @ Sun, 21 Jun 2020 21:18:21: 10000000 INFO @ Sun, 21 Jun 2020 21:18:21: 14000000 INFO @ Sun, 21 Jun 2020 21:18:26: 6000000 INFO @ Sun, 21 Jun 2020 21:18:27: 11000000 INFO @ Sun, 21 Jun 2020 21:18:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:18:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:18:27: #1 total tags in treatment: 14842892 INFO @ Sun, 21 Jun 2020 21:18:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:28: #1 tags after filtering in treatment: 14842891 INFO @ Sun, 21 Jun 2020 21:18:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:29: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 21:18:29: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:18:32: 7000000 INFO @ Sun, 21 Jun 2020 21:18:33: 12000000 INFO @ Sun, 21 Jun 2020 21:18:38: 8000000 INFO @ Sun, 21 Jun 2020 21:18:39: 13000000 INFO @ Sun, 21 Jun 2020 21:18:44: 9000000 INFO @ Sun, 21 Jun 2020 21:18:45: 14000000 INFO @ Sun, 21 Jun 2020 21:18:50: 10000000 INFO @ Sun, 21 Jun 2020 21:18:51: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:18:51: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:18:51: #1 total tags in treatment: 14842892 INFO @ Sun, 21 Jun 2020 21:18:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:51: #1 tags after filtering in treatment: 14842891 INFO @ Sun, 21 Jun 2020 21:18:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:52: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 21:18:52: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:18:55: 11000000 INFO @ Sun, 21 Jun 2020 21:19:01: 12000000 INFO @ Sun, 21 Jun 2020 21:19:07: 13000000 INFO @ Sun, 21 Jun 2020 21:19:12: 14000000 INFO @ Sun, 21 Jun 2020 21:19:17: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:19:17: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:19:17: #1 total tags in treatment: 14842892 INFO @ Sun, 21 Jun 2020 21:19:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:19:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:19:18: #1 tags after filtering in treatment: 14842891 INFO @ Sun, 21 Jun 2020 21:19:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:19:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:19:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:19:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:19:18: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 21:19:18: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:19:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495165/SRX495165.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。