Job ID = 6457983 SRX = SRX4933890 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:15:37 prefetch.2.10.7: 1) Downloading 'SRR8107280'... 2020-06-21T12:15:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:27:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:27:00 prefetch.2.10.7: 1) 'SRR8107280' was downloaded successfully Read 76602117 spots for SRR8107280/SRR8107280.sra Written 76602117 spots for SRR8107280/SRR8107280.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:01 76602117 reads; of these: 76602117 (100.00%) were unpaired; of these: 2746501 (3.59%) aligned 0 times 51454162 (67.17%) aligned exactly 1 time 22401454 (29.24%) aligned >1 times 96.41% overall alignment rate Time searching: 00:25:01 Overall time: 00:25:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 21877541 / 73855616 = 0.2962 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:15:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:15:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:15:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:15:57: 1000000 INFO @ Sun, 21 Jun 2020 22:16:02: 2000000 INFO @ Sun, 21 Jun 2020 22:16:07: 3000000 INFO @ Sun, 21 Jun 2020 22:16:12: 4000000 INFO @ Sun, 21 Jun 2020 22:16:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:16:22: 6000000 INFO @ Sun, 21 Jun 2020 22:16:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:16:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:16:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:16:27: 7000000 INFO @ Sun, 21 Jun 2020 22:16:28: 1000000 INFO @ Sun, 21 Jun 2020 22:16:33: 8000000 INFO @ Sun, 21 Jun 2020 22:16:34: 2000000 INFO @ Sun, 21 Jun 2020 22:16:38: 9000000 INFO @ Sun, 21 Jun 2020 22:16:40: 3000000 INFO @ Sun, 21 Jun 2020 22:16:44: 10000000 INFO @ Sun, 21 Jun 2020 22:16:46: 4000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 22:16:50: 11000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:16:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:16:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:16:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:16:53: 5000000 INFO @ Sun, 21 Jun 2020 22:16:56: 12000000 INFO @ Sun, 21 Jun 2020 22:16:59: 1000000 INFO @ Sun, 21 Jun 2020 22:16:59: 6000000 INFO @ Sun, 21 Jun 2020 22:17:02: 13000000 INFO @ Sun, 21 Jun 2020 22:17:05: 2000000 INFO @ Sun, 21 Jun 2020 22:17:06: 7000000 INFO @ Sun, 21 Jun 2020 22:17:07: 14000000 INFO @ Sun, 21 Jun 2020 22:17:11: 3000000 INFO @ Sun, 21 Jun 2020 22:17:12: 8000000 INFO @ Sun, 21 Jun 2020 22:17:13: 15000000 INFO @ Sun, 21 Jun 2020 22:17:17: 4000000 INFO @ Sun, 21 Jun 2020 22:17:18: 9000000 INFO @ Sun, 21 Jun 2020 22:17:19: 16000000 INFO @ Sun, 21 Jun 2020 22:17:23: 5000000 INFO @ Sun, 21 Jun 2020 22:17:25: 10000000 INFO @ Sun, 21 Jun 2020 22:17:25: 17000000 INFO @ Sun, 21 Jun 2020 22:17:30: 6000000 INFO @ Sun, 21 Jun 2020 22:17:31: 11000000 INFO @ Sun, 21 Jun 2020 22:17:31: 18000000 INFO @ Sun, 21 Jun 2020 22:17:36: 7000000 INFO @ Sun, 21 Jun 2020 22:17:37: 19000000 INFO @ Sun, 21 Jun 2020 22:17:38: 12000000 INFO @ Sun, 21 Jun 2020 22:17:42: 8000000 INFO @ Sun, 21 Jun 2020 22:17:43: 20000000 INFO @ Sun, 21 Jun 2020 22:17:44: 13000000 INFO @ Sun, 21 Jun 2020 22:17:49: 9000000 INFO @ Sun, 21 Jun 2020 22:17:49: 21000000 INFO @ Sun, 21 Jun 2020 22:17:50: 14000000 INFO @ Sun, 21 Jun 2020 22:17:55: 10000000 INFO @ Sun, 21 Jun 2020 22:17:55: 22000000 INFO @ Sun, 21 Jun 2020 22:17:56: 15000000 INFO @ Sun, 21 Jun 2020 22:18:02: 11000000 INFO @ Sun, 21 Jun 2020 22:18:02: 23000000 INFO @ Sun, 21 Jun 2020 22:18:03: 16000000 INFO @ Sun, 21 Jun 2020 22:18:08: 24000000 INFO @ Sun, 21 Jun 2020 22:18:08: 12000000 INFO @ Sun, 21 Jun 2020 22:18:09: 17000000 INFO @ Sun, 21 Jun 2020 22:18:14: 25000000 INFO @ Sun, 21 Jun 2020 22:18:14: 13000000 INFO @ Sun, 21 Jun 2020 22:18:15: 18000000 INFO @ Sun, 21 Jun 2020 22:18:20: 26000000 INFO @ Sun, 21 Jun 2020 22:18:21: 14000000 INFO @ Sun, 21 Jun 2020 22:18:21: 19000000 INFO @ Sun, 21 Jun 2020 22:18:26: 27000000 INFO @ Sun, 21 Jun 2020 22:18:27: 15000000 INFO @ Sun, 21 Jun 2020 22:18:28: 20000000 INFO @ Sun, 21 Jun 2020 22:18:32: 28000000 INFO @ Sun, 21 Jun 2020 22:18:33: 16000000 INFO @ Sun, 21 Jun 2020 22:18:34: 21000000 INFO @ Sun, 21 Jun 2020 22:18:38: 29000000 INFO @ Sun, 21 Jun 2020 22:18:40: 17000000 INFO @ Sun, 21 Jun 2020 22:18:40: 22000000 INFO @ Sun, 21 Jun 2020 22:18:44: 30000000 INFO @ Sun, 21 Jun 2020 22:18:46: 18000000 INFO @ Sun, 21 Jun 2020 22:18:47: 23000000 INFO @ Sun, 21 Jun 2020 22:18:50: 31000000 INFO @ Sun, 21 Jun 2020 22:18:52: 19000000 INFO @ Sun, 21 Jun 2020 22:18:53: 24000000 INFO @ Sun, 21 Jun 2020 22:18:56: 32000000 INFO @ Sun, 21 Jun 2020 22:18:59: 20000000 INFO @ Sun, 21 Jun 2020 22:19:00: 25000000 INFO @ Sun, 21 Jun 2020 22:19:02: 33000000 INFO @ Sun, 21 Jun 2020 22:19:05: 21000000 INFO @ Sun, 21 Jun 2020 22:19:06: 26000000 INFO @ Sun, 21 Jun 2020 22:19:08: 34000000 INFO @ Sun, 21 Jun 2020 22:19:12: 22000000 INFO @ Sun, 21 Jun 2020 22:19:12: 27000000 INFO @ Sun, 21 Jun 2020 22:19:14: 35000000 INFO @ Sun, 21 Jun 2020 22:19:18: 23000000 INFO @ Sun, 21 Jun 2020 22:19:19: 28000000 INFO @ Sun, 21 Jun 2020 22:19:20: 36000000 INFO @ Sun, 21 Jun 2020 22:19:25: 24000000 INFO @ Sun, 21 Jun 2020 22:19:25: 29000000 INFO @ Sun, 21 Jun 2020 22:19:27: 37000000 INFO @ Sun, 21 Jun 2020 22:19:31: 25000000 INFO @ Sun, 21 Jun 2020 22:19:31: 30000000 INFO @ Sun, 21 Jun 2020 22:19:33: 38000000 INFO @ Sun, 21 Jun 2020 22:19:37: 26000000 INFO @ Sun, 21 Jun 2020 22:19:38: 31000000 INFO @ Sun, 21 Jun 2020 22:19:39: 39000000 INFO @ Sun, 21 Jun 2020 22:19:44: 27000000 INFO @ Sun, 21 Jun 2020 22:19:44: 32000000 INFO @ Sun, 21 Jun 2020 22:19:45: 40000000 INFO @ Sun, 21 Jun 2020 22:19:50: 28000000 INFO @ Sun, 21 Jun 2020 22:19:50: 33000000 INFO @ Sun, 21 Jun 2020 22:19:51: 41000000 INFO @ Sun, 21 Jun 2020 22:19:56: 29000000 INFO @ Sun, 21 Jun 2020 22:19:57: 34000000 INFO @ Sun, 21 Jun 2020 22:19:57: 42000000 INFO @ Sun, 21 Jun 2020 22:20:03: 30000000 INFO @ Sun, 21 Jun 2020 22:20:03: 43000000 INFO @ Sun, 21 Jun 2020 22:20:03: 35000000 INFO @ Sun, 21 Jun 2020 22:20:09: 44000000 INFO @ Sun, 21 Jun 2020 22:20:09: 36000000 INFO @ Sun, 21 Jun 2020 22:20:09: 31000000 INFO @ Sun, 21 Jun 2020 22:20:14: 45000000 INFO @ Sun, 21 Jun 2020 22:20:15: 37000000 INFO @ Sun, 21 Jun 2020 22:20:16: 32000000 INFO @ Sun, 21 Jun 2020 22:20:20: 46000000 INFO @ Sun, 21 Jun 2020 22:20:21: 38000000 INFO @ Sun, 21 Jun 2020 22:20:22: 33000000 INFO @ Sun, 21 Jun 2020 22:20:26: 47000000 INFO @ Sun, 21 Jun 2020 22:20:28: 39000000 INFO @ Sun, 21 Jun 2020 22:20:28: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:20:32: 48000000 INFO @ Sun, 21 Jun 2020 22:20:34: 40000000 INFO @ Sun, 21 Jun 2020 22:20:35: 35000000 INFO @ Sun, 21 Jun 2020 22:20:38: 49000000 INFO @ Sun, 21 Jun 2020 22:20:40: 41000000 INFO @ Sun, 21 Jun 2020 22:20:41: 36000000 INFO @ Sun, 21 Jun 2020 22:20:45: 50000000 INFO @ Sun, 21 Jun 2020 22:20:47: 42000000 INFO @ Sun, 21 Jun 2020 22:20:48: 37000000 INFO @ Sun, 21 Jun 2020 22:20:51: 51000000 INFO @ Sun, 21 Jun 2020 22:20:54: 43000000 INFO @ Sun, 21 Jun 2020 22:20:54: 38000000 INFO @ Sun, 21 Jun 2020 22:20:58: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:20:58: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:20:58: #1 total tags in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:20:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:20:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:20:59: #1 tags after filtering in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:20:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:20:59: #1 finished! INFO @ Sun, 21 Jun 2020 22:20:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:21:00: 44000000 INFO @ Sun, 21 Jun 2020 22:21:01: 39000000 INFO @ Sun, 21 Jun 2020 22:21:03: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:21:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:21:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:21:07: 45000000 INFO @ Sun, 21 Jun 2020 22:21:07: 40000000 INFO @ Sun, 21 Jun 2020 22:21:13: 46000000 INFO @ Sun, 21 Jun 2020 22:21:14: 41000000 INFO @ Sun, 21 Jun 2020 22:21:19: 47000000 INFO @ Sun, 21 Jun 2020 22:21:21: 42000000 INFO @ Sun, 21 Jun 2020 22:21:25: 48000000 INFO @ Sun, 21 Jun 2020 22:21:28: 43000000 INFO @ Sun, 21 Jun 2020 22:21:32: 49000000 INFO @ Sun, 21 Jun 2020 22:21:34: 44000000 INFO @ Sun, 21 Jun 2020 22:21:38: 50000000 INFO @ Sun, 21 Jun 2020 22:21:40: 45000000 INFO @ Sun, 21 Jun 2020 22:21:45: 51000000 INFO @ Sun, 21 Jun 2020 22:21:46: 46000000 INFO @ Sun, 21 Jun 2020 22:21:52: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:21:52: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:21:52: #1 total tags in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:21:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:21:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:21:53: 47000000 INFO @ Sun, 21 Jun 2020 22:21:53: #1 tags after filtering in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:21:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:21:53: #1 finished! INFO @ Sun, 21 Jun 2020 22:21:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:21:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:21:56: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:21:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:21:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:21:59: 48000000 INFO @ Sun, 21 Jun 2020 22:22:05: 49000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:22:11: 50000000 INFO @ Sun, 21 Jun 2020 22:22:18: 51000000 INFO @ Sun, 21 Jun 2020 22:22:24: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:22:24: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:22:24: #1 total tags in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:22:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:26: #1 tags after filtering in treatment: 51978075 INFO @ Sun, 21 Jun 2020 22:22:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:26: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:29: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:22:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933890/SRX4933890.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling