Job ID = 6457966 SRX = SRX4933874 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:20:07 prefetch.2.10.7: 1) Downloading 'SRR8107263'... 2020-06-21T12:20:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:31:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:31:12 prefetch.2.10.7: 1) 'SRR8107263' was downloaded successfully Read 80130616 spots for SRR8107263/SRR8107263.sra Written 80130616 spots for SRR8107263/SRR8107263.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:10 80130616 reads; of these: 80130616 (100.00%) were unpaired; of these: 2479388 (3.09%) aligned 0 times 54313585 (67.78%) aligned exactly 1 time 23337643 (29.12%) aligned >1 times 96.91% overall alignment rate Time searching: 00:26:10 Overall time: 00:26:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 25588202 / 77651228 = 0.3295 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:19:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:19:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:19:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:19:57: 1000000 INFO @ Sun, 21 Jun 2020 22:20:02: 2000000 INFO @ Sun, 21 Jun 2020 22:20:08: 3000000 INFO @ Sun, 21 Jun 2020 22:20:13: 4000000 INFO @ Sun, 21 Jun 2020 22:20:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:25: 6000000 INFO @ Sun, 21 Jun 2020 22:20:28: 1000000 INFO @ Sun, 21 Jun 2020 22:20:31: 7000000 INFO @ Sun, 21 Jun 2020 22:20:35: 2000000 INFO @ Sun, 21 Jun 2020 22:20:37: 8000000 INFO @ Sun, 21 Jun 2020 22:20:42: 3000000 INFO @ Sun, 21 Jun 2020 22:20:43: 9000000 INFO @ Sun, 21 Jun 2020 22:20:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:50: 10000000 INFO @ Sun, 21 Jun 2020 22:20:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:55: 5000000 INFO @ Sun, 21 Jun 2020 22:20:56: 11000000 INFO @ Sun, 21 Jun 2020 22:20:58: 1000000 INFO @ Sun, 21 Jun 2020 22:21:02: 6000000 INFO @ Sun, 21 Jun 2020 22:21:02: 12000000 INFO @ Sun, 21 Jun 2020 22:21:05: 2000000 INFO @ Sun, 21 Jun 2020 22:21:09: 13000000 INFO @ Sun, 21 Jun 2020 22:21:09: 7000000 INFO @ Sun, 21 Jun 2020 22:21:12: 3000000 INFO @ Sun, 21 Jun 2020 22:21:15: 14000000 INFO @ Sun, 21 Jun 2020 22:21:16: 8000000 INFO @ Sun, 21 Jun 2020 22:21:19: 4000000 INFO @ Sun, 21 Jun 2020 22:21:22: 15000000 INFO @ Sun, 21 Jun 2020 22:21:23: 9000000 INFO @ Sun, 21 Jun 2020 22:21:26: 5000000 INFO @ Sun, 21 Jun 2020 22:21:28: 16000000 INFO @ Sun, 21 Jun 2020 22:21:30: 10000000 INFO @ Sun, 21 Jun 2020 22:21:33: 6000000 INFO @ Sun, 21 Jun 2020 22:21:35: 17000000 INFO @ Sun, 21 Jun 2020 22:21:37: 11000000 INFO @ Sun, 21 Jun 2020 22:21:40: 7000000 INFO @ Sun, 21 Jun 2020 22:21:41: 18000000 INFO @ Sun, 21 Jun 2020 22:21:44: 12000000 INFO @ Sun, 21 Jun 2020 22:21:47: 8000000 INFO @ Sun, 21 Jun 2020 22:21:48: 19000000 INFO @ Sun, 21 Jun 2020 22:21:51: 13000000 INFO @ Sun, 21 Jun 2020 22:21:54: 9000000 INFO @ Sun, 21 Jun 2020 22:21:55: 20000000 INFO @ Sun, 21 Jun 2020 22:21:58: 14000000 INFO @ Sun, 21 Jun 2020 22:22:01: 10000000 INFO @ Sun, 21 Jun 2020 22:22:01: 21000000 INFO @ Sun, 21 Jun 2020 22:22:05: 15000000 INFO @ Sun, 21 Jun 2020 22:22:08: 11000000 INFO @ Sun, 21 Jun 2020 22:22:08: 22000000 INFO @ Sun, 21 Jun 2020 22:22:13: 16000000 INFO @ Sun, 21 Jun 2020 22:22:15: 12000000 INFO @ Sun, 21 Jun 2020 22:22:15: 23000000 INFO @ Sun, 21 Jun 2020 22:22:20: 17000000 INFO @ Sun, 21 Jun 2020 22:22:21: 24000000 INFO @ Sun, 21 Jun 2020 22:22:22: 13000000 INFO @ Sun, 21 Jun 2020 22:22:27: 18000000 INFO @ Sun, 21 Jun 2020 22:22:28: 25000000 INFO @ Sun, 21 Jun 2020 22:22:29: 14000000 INFO @ Sun, 21 Jun 2020 22:22:35: 19000000 INFO @ Sun, 21 Jun 2020 22:22:35: 26000000 INFO @ Sun, 21 Jun 2020 22:22:35: 15000000 INFO @ Sun, 21 Jun 2020 22:22:41: 27000000 INFO @ Sun, 21 Jun 2020 22:22:42: 20000000 INFO @ Sun, 21 Jun 2020 22:22:43: 16000000 INFO @ Sun, 21 Jun 2020 22:22:48: 28000000 INFO @ Sun, 21 Jun 2020 22:22:49: 21000000 INFO @ Sun, 21 Jun 2020 22:22:50: 17000000 INFO @ Sun, 21 Jun 2020 22:22:54: 29000000 INFO @ Sun, 21 Jun 2020 22:22:57: 18000000 INFO @ Sun, 21 Jun 2020 22:22:57: 22000000 INFO @ Sun, 21 Jun 2020 22:23:01: 30000000 INFO @ Sun, 21 Jun 2020 22:23:04: 19000000 INFO @ Sun, 21 Jun 2020 22:23:04: 23000000 INFO @ Sun, 21 Jun 2020 22:23:08: 31000000 INFO @ Sun, 21 Jun 2020 22:23:11: 20000000 INFO @ Sun, 21 Jun 2020 22:23:12: 24000000 INFO @ Sun, 21 Jun 2020 22:23:15: 32000000 INFO @ Sun, 21 Jun 2020 22:23:18: 21000000 INFO @ Sun, 21 Jun 2020 22:23:19: 25000000 INFO @ Sun, 21 Jun 2020 22:23:21: 33000000 INFO @ Sun, 21 Jun 2020 22:23:25: 22000000 INFO @ Sun, 21 Jun 2020 22:23:26: 26000000 INFO @ Sun, 21 Jun 2020 22:23:28: 34000000 INFO @ Sun, 21 Jun 2020 22:23:33: 23000000 INFO @ Sun, 21 Jun 2020 22:23:34: 27000000 INFO @ Sun, 21 Jun 2020 22:23:34: 35000000 INFO @ Sun, 21 Jun 2020 22:23:40: 24000000 INFO @ Sun, 21 Jun 2020 22:23:41: 36000000 INFO @ Sun, 21 Jun 2020 22:23:41: 28000000 INFO @ Sun, 21 Jun 2020 22:23:47: 25000000 INFO @ Sun, 21 Jun 2020 22:23:47: 37000000 INFO @ Sun, 21 Jun 2020 22:23:48: 29000000 INFO @ Sun, 21 Jun 2020 22:23:54: 26000000 INFO @ Sun, 21 Jun 2020 22:23:54: 38000000 INFO @ Sun, 21 Jun 2020 22:23:56: 30000000 INFO @ Sun, 21 Jun 2020 22:24:00: 39000000 INFO @ Sun, 21 Jun 2020 22:24:01: 27000000 INFO @ Sun, 21 Jun 2020 22:24:03: 31000000 INFO @ Sun, 21 Jun 2020 22:24:07: 40000000 INFO @ Sun, 21 Jun 2020 22:24:08: 28000000 INFO @ Sun, 21 Jun 2020 22:24:11: 32000000 INFO @ Sun, 21 Jun 2020 22:24:13: 41000000 INFO @ Sun, 21 Jun 2020 22:24:15: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:24:18: 33000000 INFO @ Sun, 21 Jun 2020 22:24:20: 42000000 INFO @ Sun, 21 Jun 2020 22:24:22: 30000000 INFO @ Sun, 21 Jun 2020 22:24:25: 34000000 INFO @ Sun, 21 Jun 2020 22:24:27: 43000000 INFO @ Sun, 21 Jun 2020 22:24:29: 31000000 INFO @ Sun, 21 Jun 2020 22:24:32: 35000000 INFO @ Sun, 21 Jun 2020 22:24:34: 44000000 INFO @ Sun, 21 Jun 2020 22:24:36: 32000000 INFO @ Sun, 21 Jun 2020 22:24:39: 36000000 INFO @ Sun, 21 Jun 2020 22:24:40: 45000000 INFO @ Sun, 21 Jun 2020 22:24:43: 33000000 INFO @ Sun, 21 Jun 2020 22:24:46: 37000000 INFO @ Sun, 21 Jun 2020 22:24:47: 46000000 INFO @ Sun, 21 Jun 2020 22:24:50: 34000000 INFO @ Sun, 21 Jun 2020 22:24:53: 38000000 INFO @ Sun, 21 Jun 2020 22:24:54: 47000000 INFO @ Sun, 21 Jun 2020 22:24:57: 35000000 INFO @ Sun, 21 Jun 2020 22:25:00: 39000000 INFO @ Sun, 21 Jun 2020 22:25:01: 48000000 INFO @ Sun, 21 Jun 2020 22:25:04: 36000000 INFO @ Sun, 21 Jun 2020 22:25:07: 40000000 INFO @ Sun, 21 Jun 2020 22:25:07: 49000000 INFO @ Sun, 21 Jun 2020 22:25:11: 37000000 INFO @ Sun, 21 Jun 2020 22:25:14: 41000000 INFO @ Sun, 21 Jun 2020 22:25:14: 50000000 INFO @ Sun, 21 Jun 2020 22:25:18: 38000000 INFO @ Sun, 21 Jun 2020 22:25:21: 51000000 INFO @ Sun, 21 Jun 2020 22:25:21: 42000000 INFO @ Sun, 21 Jun 2020 22:25:25: 39000000 INFO @ Sun, 21 Jun 2020 22:25:28: 52000000 INFO @ Sun, 21 Jun 2020 22:25:28: 43000000 INFO @ Sun, 21 Jun 2020 22:25:29: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:25:29: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:25:29: #1 total tags in treatment: 52063026 INFO @ Sun, 21 Jun 2020 22:25:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:25:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:25:30: #1 tags after filtering in treatment: 52063025 INFO @ Sun, 21 Jun 2020 22:25:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:25:30: #1 finished! INFO @ Sun, 21 Jun 2020 22:25:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:25:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:25:32: 40000000 INFO @ Sun, 21 Jun 2020 22:25:33: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:25:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:25:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:25:35: 44000000 INFO @ Sun, 21 Jun 2020 22:25:39: 41000000 INFO @ Sun, 21 Jun 2020 22:25:42: 45000000 INFO @ Sun, 21 Jun 2020 22:25:46: 42000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:25:50: 46000000 INFO @ Sun, 21 Jun 2020 22:25:53: 43000000 INFO @ Sun, 21 Jun 2020 22:25:57: 47000000 INFO @ Sun, 21 Jun 2020 22:26:00: 44000000 INFO @ Sun, 21 Jun 2020 22:26:04: 48000000 INFO @ Sun, 21 Jun 2020 22:26:08: 45000000 INFO @ Sun, 21 Jun 2020 22:26:11: 49000000 INFO @ Sun, 21 Jun 2020 22:26:15: 46000000 INFO @ Sun, 21 Jun 2020 22:26:18: 50000000 INFO @ Sun, 21 Jun 2020 22:26:22: 47000000 INFO @ Sun, 21 Jun 2020 22:26:26: 51000000 INFO @ Sun, 21 Jun 2020 22:26:29: 48000000 INFO @ Sun, 21 Jun 2020 22:26:33: 52000000 INFO @ Sun, 21 Jun 2020 22:26:33: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:26:33: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:26:33: #1 total tags in treatment: 52063026 INFO @ Sun, 21 Jun 2020 22:26:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:26:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:26:35: #1 tags after filtering in treatment: 52063025 INFO @ Sun, 21 Jun 2020 22:26:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:26:35: #1 finished! INFO @ Sun, 21 Jun 2020 22:26:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:26:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:26:36: 49000000 INFO @ Sun, 21 Jun 2020 22:26:38: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:26:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:26:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:26:43: 50000000 INFO @ Sun, 21 Jun 2020 22:26:50: 51000000 INFO @ Sun, 21 Jun 2020 22:26:57: 52000000 INFO @ Sun, 21 Jun 2020 22:26:57: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:26:57: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:26:57: #1 total tags in treatment: 52063026 INFO @ Sun, 21 Jun 2020 22:26:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:26:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:26:58: #1 tags after filtering in treatment: 52063025 INFO @ Sun, 21 Jun 2020 22:26:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:26:58: #1 finished! INFO @ Sun, 21 Jun 2020 22:26:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:26:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:27:01: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:27:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:27:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933874/SRX4933874.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling