Job ID = 6457964 SRX = SRX4933872 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:08:09 prefetch.2.10.7: 1) Downloading 'SRR8107261'... 2020-06-21T12:08:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:19:39 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:19:39 prefetch.2.10.7: 1) 'SRR8107261' was downloaded successfully Read 82377252 spots for SRR8107261/SRR8107261.sra Written 82377252 spots for SRR8107261/SRR8107261.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:00 82377252 reads; of these: 82377252 (100.00%) were unpaired; of these: 2453343 (2.98%) aligned 0 times 56134607 (68.14%) aligned exactly 1 time 23789302 (28.88%) aligned >1 times 97.02% overall alignment rate Time searching: 00:26:00 Overall time: 00:26:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 31775024 / 79923909 = 0.3976 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:07: 1000000 INFO @ Sun, 21 Jun 2020 22:08:13: 2000000 INFO @ Sun, 21 Jun 2020 22:08:19: 3000000 INFO @ Sun, 21 Jun 2020 22:08:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:31: 5000000 INFO @ Sun, 21 Jun 2020 22:08:36: 1000000 INFO @ Sun, 21 Jun 2020 22:08:38: 6000000 INFO @ Sun, 21 Jun 2020 22:08:42: 2000000 INFO @ Sun, 21 Jun 2020 22:08:44: 7000000 INFO @ Sun, 21 Jun 2020 22:08:48: 3000000 INFO @ Sun, 21 Jun 2020 22:08:51: 8000000 INFO @ Sun, 21 Jun 2020 22:08:53: 4000000 INFO @ Sun, 21 Jun 2020 22:08:57: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:59: 5000000 INFO @ Sun, 21 Jun 2020 22:09:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:09:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:09:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:09:04: 10000000 INFO @ Sun, 21 Jun 2020 22:09:05: 6000000 INFO @ Sun, 21 Jun 2020 22:09:07: 1000000 INFO @ Sun, 21 Jun 2020 22:09:11: 11000000 INFO @ Sun, 21 Jun 2020 22:09:11: 7000000 INFO @ Sun, 21 Jun 2020 22:09:14: 2000000 INFO @ Sun, 21 Jun 2020 22:09:17: 8000000 INFO @ Sun, 21 Jun 2020 22:09:17: 12000000 INFO @ Sun, 21 Jun 2020 22:09:21: 3000000 INFO @ Sun, 21 Jun 2020 22:09:23: 9000000 INFO @ Sun, 21 Jun 2020 22:09:24: 13000000 INFO @ Sun, 21 Jun 2020 22:09:27: 4000000 INFO @ Sun, 21 Jun 2020 22:09:29: 10000000 INFO @ Sun, 21 Jun 2020 22:09:31: 14000000 INFO @ Sun, 21 Jun 2020 22:09:34: 5000000 INFO @ Sun, 21 Jun 2020 22:09:35: 11000000 INFO @ Sun, 21 Jun 2020 22:09:38: 15000000 INFO @ Sun, 21 Jun 2020 22:09:41: 6000000 INFO @ Sun, 21 Jun 2020 22:09:41: 12000000 INFO @ Sun, 21 Jun 2020 22:09:45: 16000000 INFO @ Sun, 21 Jun 2020 22:09:48: 13000000 INFO @ Sun, 21 Jun 2020 22:09:48: 7000000 INFO @ Sun, 21 Jun 2020 22:09:52: 17000000 INFO @ Sun, 21 Jun 2020 22:09:54: 14000000 INFO @ Sun, 21 Jun 2020 22:09:55: 8000000 INFO @ Sun, 21 Jun 2020 22:09:58: 18000000 INFO @ Sun, 21 Jun 2020 22:10:00: 15000000 INFO @ Sun, 21 Jun 2020 22:10:02: 9000000 INFO @ Sun, 21 Jun 2020 22:10:05: 19000000 INFO @ Sun, 21 Jun 2020 22:10:06: 16000000 INFO @ Sun, 21 Jun 2020 22:10:09: 10000000 INFO @ Sun, 21 Jun 2020 22:10:12: 20000000 INFO @ Sun, 21 Jun 2020 22:10:12: 17000000 INFO @ Sun, 21 Jun 2020 22:10:15: 11000000 INFO @ Sun, 21 Jun 2020 22:10:18: 18000000 INFO @ Sun, 21 Jun 2020 22:10:19: 21000000 INFO @ Sun, 21 Jun 2020 22:10:22: 12000000 INFO @ Sun, 21 Jun 2020 22:10:24: 19000000 INFO @ Sun, 21 Jun 2020 22:10:26: 22000000 INFO @ Sun, 21 Jun 2020 22:10:29: 13000000 INFO @ Sun, 21 Jun 2020 22:10:30: 20000000 INFO @ Sun, 21 Jun 2020 22:10:33: 23000000 INFO @ Sun, 21 Jun 2020 22:10:36: 14000000 INFO @ Sun, 21 Jun 2020 22:10:36: 21000000 INFO @ Sun, 21 Jun 2020 22:10:40: 24000000 INFO @ Sun, 21 Jun 2020 22:10:43: 22000000 INFO @ Sun, 21 Jun 2020 22:10:43: 15000000 INFO @ Sun, 21 Jun 2020 22:10:47: 25000000 INFO @ Sun, 21 Jun 2020 22:10:49: 23000000 INFO @ Sun, 21 Jun 2020 22:10:50: 16000000 INFO @ Sun, 21 Jun 2020 22:10:53: 26000000 INFO @ Sun, 21 Jun 2020 22:10:55: 24000000 INFO @ Sun, 21 Jun 2020 22:10:57: 17000000 INFO @ Sun, 21 Jun 2020 22:11:00: 27000000 INFO @ Sun, 21 Jun 2020 22:11:01: 25000000 INFO @ Sun, 21 Jun 2020 22:11:03: 18000000 INFO @ Sun, 21 Jun 2020 22:11:07: 26000000 INFO @ Sun, 21 Jun 2020 22:11:07: 28000000 INFO @ Sun, 21 Jun 2020 22:11:10: 19000000 INFO @ Sun, 21 Jun 2020 22:11:13: 27000000 INFO @ Sun, 21 Jun 2020 22:11:14: 29000000 INFO @ Sun, 21 Jun 2020 22:11:17: 20000000 INFO @ Sun, 21 Jun 2020 22:11:19: 28000000 INFO @ Sun, 21 Jun 2020 22:11:21: 30000000 INFO @ Sun, 21 Jun 2020 22:11:24: 21000000 INFO @ Sun, 21 Jun 2020 22:11:25: 29000000 INFO @ Sun, 21 Jun 2020 22:11:28: 31000000 INFO @ Sun, 21 Jun 2020 22:11:31: 30000000 INFO @ Sun, 21 Jun 2020 22:11:31: 22000000 INFO @ Sun, 21 Jun 2020 22:11:35: 32000000 INFO @ Sun, 21 Jun 2020 22:11:37: 31000000 INFO @ Sun, 21 Jun 2020 22:11:38: 23000000 INFO @ Sun, 21 Jun 2020 22:11:42: 33000000 INFO @ Sun, 21 Jun 2020 22:11:44: 32000000 INFO @ Sun, 21 Jun 2020 22:11:45: 24000000 INFO @ Sun, 21 Jun 2020 22:11:49: 34000000 INFO @ Sun, 21 Jun 2020 22:11:50: 33000000 INFO @ Sun, 21 Jun 2020 22:11:52: 25000000 INFO @ Sun, 21 Jun 2020 22:11:56: 35000000 INFO @ Sun, 21 Jun 2020 22:11:56: 34000000 INFO @ Sun, 21 Jun 2020 22:11:59: 26000000 INFO @ Sun, 21 Jun 2020 22:12:02: 35000000 INFO @ Sun, 21 Jun 2020 22:12:03: 36000000 INFO @ Sun, 21 Jun 2020 22:12:06: 27000000 INFO @ Sun, 21 Jun 2020 22:12:09: 36000000 INFO @ Sun, 21 Jun 2020 22:12:10: 37000000 INFO @ Sun, 21 Jun 2020 22:12:13: 28000000 INFO @ Sun, 21 Jun 2020 22:12:15: 37000000 INFO @ Sun, 21 Jun 2020 22:12:17: 38000000 INFO @ Sun, 21 Jun 2020 22:12:20: 29000000 INFO @ Sun, 21 Jun 2020 22:12:21: 38000000 INFO @ Sun, 21 Jun 2020 22:12:24: 39000000 INFO @ Sun, 21 Jun 2020 22:12:27: 30000000 INFO @ Sun, 21 Jun 2020 22:12:28: 39000000 INFO @ Sun, 21 Jun 2020 22:12:31: 40000000 INFO @ Sun, 21 Jun 2020 22:12:34: 31000000 INFO @ Sun, 21 Jun 2020 22:12:34: 40000000 INFO @ Sun, 21 Jun 2020 22:12:38: 41000000 INFO @ Sun, 21 Jun 2020 22:12:40: 41000000 INFO @ Sun, 21 Jun 2020 22:12:40: 32000000 INFO @ Sun, 21 Jun 2020 22:12:45: 42000000 INFO @ Sun, 21 Jun 2020 22:12:46: 42000000 INFO @ Sun, 21 Jun 2020 22:12:47: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:12:52: 43000000 INFO @ Sun, 21 Jun 2020 22:12:53: 43000000 INFO @ Sun, 21 Jun 2020 22:12:54: 34000000 INFO @ Sun, 21 Jun 2020 22:13:00: 44000000 INFO @ Sun, 21 Jun 2020 22:13:00: 44000000 INFO @ Sun, 21 Jun 2020 22:13:01: 35000000 INFO @ Sun, 21 Jun 2020 22:13:07: 45000000 INFO @ Sun, 21 Jun 2020 22:13:07: 45000000 INFO @ Sun, 21 Jun 2020 22:13:07: 36000000 INFO @ Sun, 21 Jun 2020 22:13:14: 46000000 INFO @ Sun, 21 Jun 2020 22:13:14: 37000000 INFO @ Sun, 21 Jun 2020 22:13:14: 46000000 INFO @ Sun, 21 Jun 2020 22:13:20: 47000000 INFO @ Sun, 21 Jun 2020 22:13:21: 38000000 INFO @ Sun, 21 Jun 2020 22:13:21: 47000000 INFO @ Sun, 21 Jun 2020 22:13:26: 48000000 INFO @ Sun, 21 Jun 2020 22:13:28: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:13:28: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:13:28: #1 total tags in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:13:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:28: 39000000 INFO @ Sun, 21 Jun 2020 22:13:28: 48000000 INFO @ Sun, 21 Jun 2020 22:13:29: #1 tags after filtering in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:13:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:29: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:30: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:13:30: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:13:30: #1 total tags in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:13:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:31: #1 tags after filtering in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:13:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:31: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:32: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:13:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:13:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:13:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:13:34: 40000000 INFO @ Sun, 21 Jun 2020 22:13:41: 41000000 INFO @ Sun, 21 Jun 2020 22:13:47: 42000000 INFO @ Sun, 21 Jun 2020 22:13:54: 43000000 INFO @ Sun, 21 Jun 2020 22:14:00: 44000000 INFO @ Sun, 21 Jun 2020 22:14:06: 45000000 INFO @ Sun, 21 Jun 2020 22:14:12: 46000000 INFO @ Sun, 21 Jun 2020 22:14:19: 47000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:14:25: 48000000 INFO @ Sun, 21 Jun 2020 22:14:26: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:14:26: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:14:26: #1 total tags in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:14:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:14:27: #1 tags after filtering in treatment: 48148885 INFO @ Sun, 21 Jun 2020 22:14:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:14:27: #1 finished! INFO @ Sun, 21 Jun 2020 22:14:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:14:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:14:30: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:14:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:14:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4933872/SRX4933872.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling