Job ID = 6529794 SRX = SRX4923198 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:42:54 114864952 reads; of these: 114864952 (100.00%) were unpaired; of these: 2204281 (1.92%) aligned 0 times 32213455 (28.04%) aligned exactly 1 time 80447216 (70.04%) aligned >1 times 98.08% overall alignment rate Time searching: 00:42:54 Overall time: 00:42:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 68131452 / 112660671 = 0.6047 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 04:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 04:09:43: #1 read tag files... INFO @ Tue, 30 Jun 2020 04:09:43: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 04:09:48: 1000000 INFO @ Tue, 30 Jun 2020 04:09:53: 2000000 INFO @ Tue, 30 Jun 2020 04:09:58: 3000000 INFO @ Tue, 30 Jun 2020 04:10:03: 4000000 INFO @ Tue, 30 Jun 2020 04:10:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 04:10:13: 6000000 INFO @ Tue, 30 Jun 2020 04:10:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 04:10:13: #1 read tag files... INFO @ Tue, 30 Jun 2020 04:10:13: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 04:10:18: 7000000 INFO @ Tue, 30 Jun 2020 04:10:19: 1000000 INFO @ Tue, 30 Jun 2020 04:10:23: 8000000 INFO @ Tue, 30 Jun 2020 04:10:25: 2000000 INFO @ Tue, 30 Jun 2020 04:10:29: 9000000 INFO @ Tue, 30 Jun 2020 04:10:30: 3000000 INFO @ Tue, 30 Jun 2020 04:10:34: 10000000 INFO @ Tue, 30 Jun 2020 04:10:35: 4000000 INFO @ Tue, 30 Jun 2020 04:10:40: 11000000 INFO @ Tue, 30 Jun 2020 04:10:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 04:10:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 04:10:43: #1 read tag files... INFO @ Tue, 30 Jun 2020 04:10:43: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 04:10:45: 12000000 INFO @ Tue, 30 Jun 2020 04:10:46: 6000000 INFO @ Tue, 30 Jun 2020 04:10:49: 1000000 INFO @ Tue, 30 Jun 2020 04:10:51: 13000000 INFO @ Tue, 30 Jun 2020 04:10:52: 7000000 INFO @ Tue, 30 Jun 2020 04:10:55: 2000000 INFO @ Tue, 30 Jun 2020 04:10:56: 14000000 INFO @ Tue, 30 Jun 2020 04:10:57: 8000000 INFO @ Tue, 30 Jun 2020 04:11:00: 3000000 INFO @ Tue, 30 Jun 2020 04:11:02: 15000000 INFO @ Tue, 30 Jun 2020 04:11:02: 9000000 INFO @ Tue, 30 Jun 2020 04:11:05: 4000000 INFO @ Tue, 30 Jun 2020 04:11:07: 16000000 INFO @ Tue, 30 Jun 2020 04:11:07: 10000000 INFO @ Tue, 30 Jun 2020 04:11:10: 5000000 INFO @ Tue, 30 Jun 2020 04:11:13: 11000000 INFO @ Tue, 30 Jun 2020 04:11:13: 17000000 INFO @ Tue, 30 Jun 2020 04:11:15: 6000000 INFO @ Tue, 30 Jun 2020 04:11:18: 12000000 INFO @ Tue, 30 Jun 2020 04:11:18: 18000000 INFO @ Tue, 30 Jun 2020 04:11:20: 7000000 INFO @ Tue, 30 Jun 2020 04:11:23: 13000000 INFO @ Tue, 30 Jun 2020 04:11:24: 19000000 INFO @ Tue, 30 Jun 2020 04:11:25: 8000000 INFO @ Tue, 30 Jun 2020 04:11:29: 14000000 INFO @ Tue, 30 Jun 2020 04:11:29: 20000000 INFO @ Tue, 30 Jun 2020 04:11:30: 9000000 INFO @ Tue, 30 Jun 2020 04:11:34: 15000000 INFO @ Tue, 30 Jun 2020 04:11:34: 21000000 INFO @ Tue, 30 Jun 2020 04:11:35: 10000000 INFO @ Tue, 30 Jun 2020 04:11:39: 16000000 INFO @ Tue, 30 Jun 2020 04:11:40: 22000000 INFO @ Tue, 30 Jun 2020 04:11:40: 11000000 INFO @ Tue, 30 Jun 2020 04:11:45: 17000000 INFO @ Tue, 30 Jun 2020 04:11:45: 23000000 INFO @ Tue, 30 Jun 2020 04:11:45: 12000000 INFO @ Tue, 30 Jun 2020 04:11:50: 18000000 INFO @ Tue, 30 Jun 2020 04:11:50: 24000000 INFO @ Tue, 30 Jun 2020 04:11:50: 13000000 INFO @ Tue, 30 Jun 2020 04:11:55: 19000000 INFO @ Tue, 30 Jun 2020 04:11:55: 14000000 INFO @ Tue, 30 Jun 2020 04:11:56: 25000000 INFO @ Tue, 30 Jun 2020 04:12:01: 15000000 INFO @ Tue, 30 Jun 2020 04:12:01: 20000000 INFO @ Tue, 30 Jun 2020 04:12:01: 26000000 INFO @ Tue, 30 Jun 2020 04:12:06: 16000000 INFO @ Tue, 30 Jun 2020 04:12:06: 21000000 INFO @ Tue, 30 Jun 2020 04:12:06: 27000000 INFO @ Tue, 30 Jun 2020 04:12:11: 17000000 INFO @ Tue, 30 Jun 2020 04:12:11: 22000000 INFO @ Tue, 30 Jun 2020 04:12:11: 28000000 INFO @ Tue, 30 Jun 2020 04:12:16: 18000000 INFO @ Tue, 30 Jun 2020 04:12:17: 23000000 INFO @ Tue, 30 Jun 2020 04:12:17: 29000000 INFO @ Tue, 30 Jun 2020 04:12:22: 19000000 INFO @ Tue, 30 Jun 2020 04:12:22: 24000000 INFO @ Tue, 30 Jun 2020 04:12:22: 30000000 INFO @ Tue, 30 Jun 2020 04:12:27: 20000000 INFO @ Tue, 30 Jun 2020 04:12:27: 25000000 INFO @ Tue, 30 Jun 2020 04:12:28: 31000000 INFO @ Tue, 30 Jun 2020 04:12:32: 21000000 INFO @ Tue, 30 Jun 2020 04:12:33: 26000000 INFO @ Tue, 30 Jun 2020 04:12:33: 32000000 INFO @ Tue, 30 Jun 2020 04:12:37: 22000000 INFO @ Tue, 30 Jun 2020 04:12:38: 27000000 INFO @ Tue, 30 Jun 2020 04:12:39: 33000000 INFO @ Tue, 30 Jun 2020 04:12:42: 23000000 INFO @ Tue, 30 Jun 2020 04:12:43: 28000000 INFO @ Tue, 30 Jun 2020 04:12:44: 34000000 INFO @ Tue, 30 Jun 2020 04:12:47: 24000000 INFO @ Tue, 30 Jun 2020 04:12:48: 29000000 INFO @ Tue, 30 Jun 2020 04:12:50: 35000000 INFO @ Tue, 30 Jun 2020 04:12:52: 25000000 INFO @ Tue, 30 Jun 2020 04:12:54: 30000000 INFO @ Tue, 30 Jun 2020 04:12:56: 36000000 INFO @ Tue, 30 Jun 2020 04:12:57: 26000000 INFO @ Tue, 30 Jun 2020 04:12:59: 31000000 INFO @ Tue, 30 Jun 2020 04:13:01: 37000000 INFO @ Tue, 30 Jun 2020 04:13:02: 27000000 INFO @ Tue, 30 Jun 2020 04:13:05: 32000000 INFO @ Tue, 30 Jun 2020 04:13:06: 38000000 INFO @ Tue, 30 Jun 2020 04:13:07: 28000000 INFO @ Tue, 30 Jun 2020 04:13:10: 33000000 INFO @ Tue, 30 Jun 2020 04:13:12: 39000000 INFO @ Tue, 30 Jun 2020 04:13:12: 29000000 INFO @ Tue, 30 Jun 2020 04:13:16: 34000000 INFO @ Tue, 30 Jun 2020 04:13:17: 40000000 INFO @ Tue, 30 Jun 2020 04:13:17: 30000000 INFO @ Tue, 30 Jun 2020 04:13:22: 35000000 INFO @ Tue, 30 Jun 2020 04:13:22: 41000000 INFO @ Tue, 30 Jun 2020 04:13:22: 31000000 INFO @ Tue, 30 Jun 2020 04:13:27: 36000000 INFO @ Tue, 30 Jun 2020 04:13:27: 32000000 INFO @ Tue, 30 Jun 2020 04:13:28: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 04:13:33: 37000000 INFO @ Tue, 30 Jun 2020 04:13:34: 33000000 INFO @ Tue, 30 Jun 2020 04:13:34: 43000000 INFO @ Tue, 30 Jun 2020 04:13:38: 38000000 INFO @ Tue, 30 Jun 2020 04:13:39: 34000000 INFO @ Tue, 30 Jun 2020 04:13:39: 44000000 INFO @ Tue, 30 Jun 2020 04:13:43: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 04:13:43: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 04:13:43: #1 total tags in treatment: 44529219 INFO @ Tue, 30 Jun 2020 04:13:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 04:13:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 04:13:43: 39000000 INFO @ Tue, 30 Jun 2020 04:13:44: #1 tags after filtering in treatment: 44529207 INFO @ Tue, 30 Jun 2020 04:13:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 04:13:44: #1 finished! INFO @ Tue, 30 Jun 2020 04:13:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 04:13:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 04:13:45: 35000000 INFO @ Tue, 30 Jun 2020 04:13:47: #2 number of paired peaks: 1927 INFO @ Tue, 30 Jun 2020 04:13:47: start model_add_line... INFO @ Tue, 30 Jun 2020 04:13:48: start X-correlation... INFO @ Tue, 30 Jun 2020 04:13:48: end of X-cor INFO @ Tue, 30 Jun 2020 04:13:48: #2 finished! INFO @ Tue, 30 Jun 2020 04:13:48: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 04:13:48: #2 alternative fragment length(s) may be 0,37,55 bps INFO @ Tue, 30 Jun 2020 04:13:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.05_model.r WARNING @ Tue, 30 Jun 2020 04:13:48: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 04:13:48: #2 You may need to consider one of the other alternative d(s): 0,37,55 WARNING @ Tue, 30 Jun 2020 04:13:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 04:13:48: #3 Call peaks... INFO @ Tue, 30 Jun 2020 04:13:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 04:13:48: 40000000 INFO @ Tue, 30 Jun 2020 04:13:50: 36000000 INFO @ Tue, 30 Jun 2020 04:13:53: 41000000 INFO @ Tue, 30 Jun 2020 04:13:55: 37000000 INFO @ Tue, 30 Jun 2020 04:13:57: 42000000 INFO @ Tue, 30 Jun 2020 04:14:00: 38000000 INFO @ Tue, 30 Jun 2020 04:14:02: 43000000 INFO @ Tue, 30 Jun 2020 04:14:05: 39000000 INFO @ Tue, 30 Jun 2020 04:14:07: 44000000 INFO @ Tue, 30 Jun 2020 04:14:10: 40000000 INFO @ Tue, 30 Jun 2020 04:14:10: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 04:14:10: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 04:14:10: #1 total tags in treatment: 44529219 INFO @ Tue, 30 Jun 2020 04:14:10: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 04:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 04:14:12: #1 tags after filtering in treatment: 44529207 INFO @ Tue, 30 Jun 2020 04:14:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 04:14:12: #1 finished! INFO @ Tue, 30 Jun 2020 04:14:12: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 04:14:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 04:14:15: #2 number of paired peaks: 1927 INFO @ Tue, 30 Jun 2020 04:14:15: start model_add_line... INFO @ Tue, 30 Jun 2020 04:14:15: start X-correlation... INFO @ Tue, 30 Jun 2020 04:14:15: end of X-cor INFO @ Tue, 30 Jun 2020 04:14:15: #2 finished! INFO @ Tue, 30 Jun 2020 04:14:15: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 04:14:15: #2 alternative fragment length(s) may be 0,37,55 bps INFO @ Tue, 30 Jun 2020 04:14:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.10_model.r WARNING @ Tue, 30 Jun 2020 04:14:15: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 04:14:15: #2 You may need to consider one of the other alternative d(s): 0,37,55 WARNING @ Tue, 30 Jun 2020 04:14:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 04:14:15: #3 Call peaks... INFO @ Tue, 30 Jun 2020 04:14:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 04:14:15: 41000000 INFO @ Tue, 30 Jun 2020 04:14:20: 42000000 INFO @ Tue, 30 Jun 2020 04:14:26: 43000000 INFO @ Tue, 30 Jun 2020 04:14:31: 44000000 INFO @ Tue, 30 Jun 2020 04:14:34: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 04:14:34: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 04:14:34: #1 total tags in treatment: 44529219 INFO @ Tue, 30 Jun 2020 04:14:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 04:14:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 04:14:36: #1 tags after filtering in treatment: 44529207 INFO @ Tue, 30 Jun 2020 04:14:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 04:14:36: #1 finished! INFO @ Tue, 30 Jun 2020 04:14:36: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 04:14:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 04:14:39: #2 number of paired peaks: 1927 INFO @ Tue, 30 Jun 2020 04:14:39: start model_add_line... INFO @ Tue, 30 Jun 2020 04:14:39: start X-correlation... INFO @ Tue, 30 Jun 2020 04:14:39: end of X-cor INFO @ Tue, 30 Jun 2020 04:14:39: #2 finished! INFO @ Tue, 30 Jun 2020 04:14:39: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 04:14:39: #2 alternative fragment length(s) may be 0,37,55 bps INFO @ Tue, 30 Jun 2020 04:14:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4923198/SRX4923198.20_model.r BigWig に変換しました。 /var/spool/uge/it024/job_scripts/6529794: line 293: 2953 Terminated MACS $i /var/spool/uge/it024/job_scripts/6529794: line 293: 18028 Terminated MACS $i /var/spool/uge/it024/job_scripts/6529794: line 293: 32208 Terminated MACS $i ls: cannot access SRX4923198.05.bed: No such file or directory mv: cannot stat ‘SRX4923198.05.bed’: No such file or directory mv: cannot stat ‘SRX4923198.05.bb’: No such file or directory ls: cannot access SRX4923198.10.bed: No such file or directory mv: cannot stat ‘SRX4923198.10.bed’: No such file or directory mv: cannot stat ‘SRX4923198.10.bb’: No such file or directory ls: cannot access SRX4923198.20.bed: No such file or directory mv: cannot stat ‘SRX4923198.20.bed’: No such file or directory mv: cannot stat ‘SRX4923198.20.bb’: No such file or directory WARNING @ Tue, 30 Jun 2020 04:14:40: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 04:14:40: #2 You may need to consider one of the other alternative d(s): 0,37,55 WARNING @ Tue, 30 Jun 2020 04:14:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 04:14:40: #3 Call peaks... INFO @ Tue, 30 Jun 2020 04:14:40: #3 Pre-compute pvalue-qvalue table...