Job ID = 6457926 SRX = SRX490732 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:11:19 prefetch.2.10.7: 1) Downloading 'SRR1193877'... 2020-06-21T12:11:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:14:18 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:14:18 prefetch.2.10.7: 1) 'SRR1193877' was downloaded successfully Read 27794555 spots for SRR1193877/SRR1193877.sra Written 27794555 spots for SRR1193877/SRR1193877.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:36 27794555 reads; of these: 27794555 (100.00%) were unpaired; of these: 1213747 (4.37%) aligned 0 times 24090330 (86.67%) aligned exactly 1 time 2490478 (8.96%) aligned >1 times 95.63% overall alignment rate Time searching: 00:04:36 Overall time: 00:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2330848 / 26580808 = 0.0877 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:27:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:27:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:27:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:27:37: 1000000 INFO @ Sun, 21 Jun 2020 21:27:42: 2000000 INFO @ Sun, 21 Jun 2020 21:27:48: 3000000 INFO @ Sun, 21 Jun 2020 21:27:53: 4000000 INFO @ Sun, 21 Jun 2020 21:27:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:28:04: 6000000 INFO @ Sun, 21 Jun 2020 21:28:07: 1000000 INFO @ Sun, 21 Jun 2020 21:28:10: 7000000 INFO @ Sun, 21 Jun 2020 21:28:12: 2000000 INFO @ Sun, 21 Jun 2020 21:28:15: 8000000 INFO @ Sun, 21 Jun 2020 21:28:18: 3000000 INFO @ Sun, 21 Jun 2020 21:28:21: 9000000 INFO @ Sun, 21 Jun 2020 21:28:24: 4000000 INFO @ Sun, 21 Jun 2020 21:28:26: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:28:29: 5000000 INFO @ Sun, 21 Jun 2020 21:28:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:28:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:28:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:28:32: 11000000 INFO @ Sun, 21 Jun 2020 21:28:35: 6000000 INFO @ Sun, 21 Jun 2020 21:28:38: 1000000 INFO @ Sun, 21 Jun 2020 21:28:38: 12000000 INFO @ Sun, 21 Jun 2020 21:28:41: 7000000 INFO @ Sun, 21 Jun 2020 21:28:43: 13000000 INFO @ Sun, 21 Jun 2020 21:28:44: 2000000 INFO @ Sun, 21 Jun 2020 21:28:47: 8000000 INFO @ Sun, 21 Jun 2020 21:28:49: 14000000 INFO @ Sun, 21 Jun 2020 21:28:50: 3000000 INFO @ Sun, 21 Jun 2020 21:28:53: 9000000 INFO @ Sun, 21 Jun 2020 21:28:55: 15000000 INFO @ Sun, 21 Jun 2020 21:28:56: 4000000 INFO @ Sun, 21 Jun 2020 21:29:00: 10000000 INFO @ Sun, 21 Jun 2020 21:29:00: 16000000 INFO @ Sun, 21 Jun 2020 21:29:02: 5000000 INFO @ Sun, 21 Jun 2020 21:29:06: 11000000 INFO @ Sun, 21 Jun 2020 21:29:06: 17000000 INFO @ Sun, 21 Jun 2020 21:29:08: 6000000 INFO @ Sun, 21 Jun 2020 21:29:11: 18000000 INFO @ Sun, 21 Jun 2020 21:29:12: 12000000 INFO @ Sun, 21 Jun 2020 21:29:15: 7000000 INFO @ Sun, 21 Jun 2020 21:29:17: 19000000 INFO @ Sun, 21 Jun 2020 21:29:18: 13000000 INFO @ Sun, 21 Jun 2020 21:29:21: 8000000 INFO @ Sun, 21 Jun 2020 21:29:24: 20000000 INFO @ Sun, 21 Jun 2020 21:29:24: 14000000 INFO @ Sun, 21 Jun 2020 21:29:27: 9000000 INFO @ Sun, 21 Jun 2020 21:29:29: 21000000 INFO @ Sun, 21 Jun 2020 21:29:30: 15000000 INFO @ Sun, 21 Jun 2020 21:29:33: 10000000 INFO @ Sun, 21 Jun 2020 21:29:35: 22000000 INFO @ Sun, 21 Jun 2020 21:29:36: 16000000 INFO @ Sun, 21 Jun 2020 21:29:39: 11000000 INFO @ Sun, 21 Jun 2020 21:29:41: 23000000 INFO @ Sun, 21 Jun 2020 21:29:43: 17000000 INFO @ Sun, 21 Jun 2020 21:29:45: 12000000 INFO @ Sun, 21 Jun 2020 21:29:46: 24000000 INFO @ Sun, 21 Jun 2020 21:29:48: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:29:48: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:29:48: #1 total tags in treatment: 24249960 INFO @ Sun, 21 Jun 2020 21:29:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:29:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:29:49: 18000000 INFO @ Sun, 21 Jun 2020 21:29:49: #1 tags after filtering in treatment: 24249905 INFO @ Sun, 21 Jun 2020 21:29:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:29:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:29:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:29:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:29:51: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 21:29:51: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:29:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:29:51: 13000000 INFO @ Sun, 21 Jun 2020 21:29:55: 19000000 INFO @ Sun, 21 Jun 2020 21:29:57: 14000000 INFO @ Sun, 21 Jun 2020 21:30:01: 20000000 INFO @ Sun, 21 Jun 2020 21:30:03: 15000000 INFO @ Sun, 21 Jun 2020 21:30:07: 21000000 INFO @ Sun, 21 Jun 2020 21:30:09: 16000000 INFO @ Sun, 21 Jun 2020 21:30:13: 22000000 INFO @ Sun, 21 Jun 2020 21:30:15: 17000000 INFO @ Sun, 21 Jun 2020 21:30:19: 23000000 INFO @ Sun, 21 Jun 2020 21:30:21: 18000000 INFO @ Sun, 21 Jun 2020 21:30:25: 24000000 INFO @ Sun, 21 Jun 2020 21:30:27: 19000000 INFO @ Sun, 21 Jun 2020 21:30:27: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:30:27: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:30:27: #1 total tags in treatment: 24249960 INFO @ Sun, 21 Jun 2020 21:30:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:30:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:30:28: #1 tags after filtering in treatment: 24249905 INFO @ Sun, 21 Jun 2020 21:30:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:30:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:30:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:30:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:30:30: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 21:30:30: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:30:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:30:33: 20000000 INFO @ Sun, 21 Jun 2020 21:30:39: 21000000 INFO @ Sun, 21 Jun 2020 21:30:44: 22000000 INFO @ Sun, 21 Jun 2020 21:30:50: 23000000 INFO @ Sun, 21 Jun 2020 21:30:55: 24000000 INFO @ Sun, 21 Jun 2020 21:30:57: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:30:57: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:30:57: #1 total tags in treatment: 24249960 INFO @ Sun, 21 Jun 2020 21:30:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:30:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:30:58: #1 tags after filtering in treatment: 24249905 INFO @ Sun, 21 Jun 2020 21:30:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:30:58: #1 finished! INFO @ Sun, 21 Jun 2020 21:30:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:30:59: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 21:30:59: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:30:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490732/SRX490732.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。