Job ID = 6457925 SRX = SRX490731 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:18:34 prefetch.2.10.7: 1) Downloading 'SRR1193876'... 2020-06-21T12:18:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:47 prefetch.2.10.7: 1) 'SRR1193876' was downloaded successfully Read 27064284 spots for SRR1193876/SRR1193876.sra Written 27064284 spots for SRR1193876/SRR1193876.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:24 27064284 reads; of these: 27064284 (100.00%) were unpaired; of these: 5078686 (18.77%) aligned 0 times 19862159 (73.39%) aligned exactly 1 time 2123439 (7.85%) aligned >1 times 81.23% overall alignment rate Time searching: 00:04:24 Overall time: 00:04:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5767925 / 21985598 = 0.2624 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:31:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:31:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:31:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:31:46: 1000000 INFO @ Sun, 21 Jun 2020 21:31:51: 2000000 INFO @ Sun, 21 Jun 2020 21:31:56: 3000000 INFO @ Sun, 21 Jun 2020 21:32:01: 4000000 INFO @ Sun, 21 Jun 2020 21:32:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:11: 6000000 INFO @ Sun, 21 Jun 2020 21:32:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:16: 7000000 INFO @ Sun, 21 Jun 2020 21:32:17: 1000000 INFO @ Sun, 21 Jun 2020 21:32:21: 8000000 INFO @ Sun, 21 Jun 2020 21:32:22: 2000000 INFO @ Sun, 21 Jun 2020 21:32:26: 9000000 INFO @ Sun, 21 Jun 2020 21:32:27: 3000000 INFO @ Sun, 21 Jun 2020 21:32:31: 10000000 INFO @ Sun, 21 Jun 2020 21:32:33: 4000000 INFO @ Sun, 21 Jun 2020 21:32:37: 11000000 INFO @ Sun, 21 Jun 2020 21:32:38: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:42: 12000000 INFO @ Sun, 21 Jun 2020 21:32:43: 6000000 INFO @ Sun, 21 Jun 2020 21:32:47: 1000000 INFO @ Sun, 21 Jun 2020 21:32:47: 13000000 INFO @ Sun, 21 Jun 2020 21:32:49: 7000000 INFO @ Sun, 21 Jun 2020 21:32:52: 2000000 INFO @ Sun, 21 Jun 2020 21:32:53: 14000000 INFO @ Sun, 21 Jun 2020 21:32:54: 8000000 INFO @ Sun, 21 Jun 2020 21:32:57: 3000000 INFO @ Sun, 21 Jun 2020 21:32:58: 15000000 INFO @ Sun, 21 Jun 2020 21:32:59: 9000000 INFO @ Sun, 21 Jun 2020 21:33:03: 4000000 INFO @ Sun, 21 Jun 2020 21:33:03: 16000000 INFO @ Sun, 21 Jun 2020 21:33:05: 10000000 INFO @ Sun, 21 Jun 2020 21:33:05: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:33:05: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:33:05: #1 total tags in treatment: 16217673 INFO @ Sun, 21 Jun 2020 21:33:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:33:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:33:05: #1 tags after filtering in treatment: 16217634 INFO @ Sun, 21 Jun 2020 21:33:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:33:05: #1 finished! INFO @ Sun, 21 Jun 2020 21:33:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:33:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:33:06: #2 number of paired peaks: 52 WARNING @ Sun, 21 Jun 2020 21:33:06: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:33:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:33:08: 5000000 INFO @ Sun, 21 Jun 2020 21:33:10: 11000000 INFO @ Sun, 21 Jun 2020 21:33:13: 6000000 INFO @ Sun, 21 Jun 2020 21:33:15: 12000000 INFO @ Sun, 21 Jun 2020 21:33:19: 7000000 INFO @ Sun, 21 Jun 2020 21:33:21: 13000000 INFO @ Sun, 21 Jun 2020 21:33:24: 8000000 INFO @ Sun, 21 Jun 2020 21:33:26: 14000000 INFO @ Sun, 21 Jun 2020 21:33:29: 9000000 INFO @ Sun, 21 Jun 2020 21:33:32: 15000000 INFO @ Sun, 21 Jun 2020 21:33:34: 10000000 INFO @ Sun, 21 Jun 2020 21:33:37: 16000000 INFO @ Sun, 21 Jun 2020 21:33:38: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:33:38: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:33:38: #1 total tags in treatment: 16217673 INFO @ Sun, 21 Jun 2020 21:33:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:33:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:33:39: #1 tags after filtering in treatment: 16217634 INFO @ Sun, 21 Jun 2020 21:33:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:33:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:33:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:33:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:33:39: 11000000 INFO @ Sun, 21 Jun 2020 21:33:40: #2 number of paired peaks: 52 WARNING @ Sun, 21 Jun 2020 21:33:40: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:33:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:33:44: 12000000 INFO @ Sun, 21 Jun 2020 21:33:49: 13000000 INFO @ Sun, 21 Jun 2020 21:33:55: 14000000 INFO @ Sun, 21 Jun 2020 21:34:00: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:34:04: 16000000 INFO @ Sun, 21 Jun 2020 21:34:06: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 21:34:06: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 21:34:06: #1 total tags in treatment: 16217673 INFO @ Sun, 21 Jun 2020 21:34:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:34:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:34:06: #1 tags after filtering in treatment: 16217634 INFO @ Sun, 21 Jun 2020 21:34:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:34:06: #1 finished! INFO @ Sun, 21 Jun 2020 21:34:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:34:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:34:07: #2 number of paired peaks: 52 WARNING @ Sun, 21 Jun 2020 21:34:07: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:34:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX490731/SRX490731.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。