Job ID = 6457891 SRX = SRX4828018 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:59:06 prefetch.2.10.7: 1) Downloading 'SRR7997154'... 2020-06-21T11:59:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:07:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:07:28 prefetch.2.10.7: 1) 'SRR7997154' was downloaded successfully 2020-06-21T12:07:28 prefetch.2.10.7: 'SRR7997154' has 0 unresolved dependencies Read 73535040 spots for SRR7997154/SRR7997154.sra Written 73535040 spots for SRR7997154/SRR7997154.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:37 73535040 reads; of these: 73535040 (100.00%) were unpaired; of these: 3630220 (4.94%) aligned 0 times 56339695 (76.62%) aligned exactly 1 time 13565125 (18.45%) aligned >1 times 95.06% overall alignment rate Time searching: 00:17:37 Overall time: 00:17:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 19422564 / 69904820 = 0.2778 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:40:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:40:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:40:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:40:15: 1000000 INFO @ Sun, 21 Jun 2020 21:40:21: 2000000 INFO @ Sun, 21 Jun 2020 21:40:27: 3000000 INFO @ Sun, 21 Jun 2020 21:40:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:40:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:40:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:40:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:40:40: 5000000 INFO @ Sun, 21 Jun 2020 21:40:45: 1000000 INFO @ Sun, 21 Jun 2020 21:40:47: 6000000 INFO @ Sun, 21 Jun 2020 21:40:52: 2000000 INFO @ Sun, 21 Jun 2020 21:40:54: 7000000 INFO @ Sun, 21 Jun 2020 21:40:59: 3000000 INFO @ Sun, 21 Jun 2020 21:41:01: 8000000 INFO @ Sun, 21 Jun 2020 21:41:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:41:08: 9000000 INFO @ Sun, 21 Jun 2020 21:41:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:41:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:41:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:41:13: 5000000 INFO @ Sun, 21 Jun 2020 21:41:15: 10000000 INFO @ Sun, 21 Jun 2020 21:41:15: 1000000 INFO @ Sun, 21 Jun 2020 21:41:20: 6000000 INFO @ Sun, 21 Jun 2020 21:41:22: 11000000 INFO @ Sun, 21 Jun 2020 21:41:23: 2000000 INFO @ Sun, 21 Jun 2020 21:41:27: 7000000 INFO @ Sun, 21 Jun 2020 21:41:30: 12000000 INFO @ Sun, 21 Jun 2020 21:41:30: 3000000 INFO @ Sun, 21 Jun 2020 21:41:34: 8000000 INFO @ Sun, 21 Jun 2020 21:41:37: 13000000 INFO @ Sun, 21 Jun 2020 21:41:37: 4000000 INFO @ Sun, 21 Jun 2020 21:41:41: 9000000 INFO @ Sun, 21 Jun 2020 21:41:44: 14000000 INFO @ Sun, 21 Jun 2020 21:41:45: 5000000 INFO @ Sun, 21 Jun 2020 21:41:48: 10000000 INFO @ Sun, 21 Jun 2020 21:41:52: 15000000 INFO @ Sun, 21 Jun 2020 21:41:52: 6000000 INFO @ Sun, 21 Jun 2020 21:41:55: 11000000 INFO @ Sun, 21 Jun 2020 21:41:59: 16000000 INFO @ Sun, 21 Jun 2020 21:41:59: 7000000 INFO @ Sun, 21 Jun 2020 21:42:02: 12000000 INFO @ Sun, 21 Jun 2020 21:42:06: 17000000 INFO @ Sun, 21 Jun 2020 21:42:06: 8000000 INFO @ Sun, 21 Jun 2020 21:42:09: 13000000 INFO @ Sun, 21 Jun 2020 21:42:13: 18000000 INFO @ Sun, 21 Jun 2020 21:42:13: 9000000 INFO @ Sun, 21 Jun 2020 21:42:16: 14000000 INFO @ Sun, 21 Jun 2020 21:42:20: 19000000 INFO @ Sun, 21 Jun 2020 21:42:21: 10000000 INFO @ Sun, 21 Jun 2020 21:42:23: 15000000 INFO @ Sun, 21 Jun 2020 21:42:28: 20000000 INFO @ Sun, 21 Jun 2020 21:42:28: 11000000 INFO @ Sun, 21 Jun 2020 21:42:30: 16000000 INFO @ Sun, 21 Jun 2020 21:42:35: 21000000 INFO @ Sun, 21 Jun 2020 21:42:35: 12000000 INFO @ Sun, 21 Jun 2020 21:42:37: 17000000 INFO @ Sun, 21 Jun 2020 21:42:42: 22000000 INFO @ Sun, 21 Jun 2020 21:42:42: 13000000 INFO @ Sun, 21 Jun 2020 21:42:44: 18000000 INFO @ Sun, 21 Jun 2020 21:42:49: 23000000 INFO @ Sun, 21 Jun 2020 21:42:50: 14000000 INFO @ Sun, 21 Jun 2020 21:42:51: 19000000 INFO @ Sun, 21 Jun 2020 21:42:57: 24000000 INFO @ Sun, 21 Jun 2020 21:42:57: 15000000 INFO @ Sun, 21 Jun 2020 21:42:58: 20000000 INFO @ Sun, 21 Jun 2020 21:43:04: 25000000 INFO @ Sun, 21 Jun 2020 21:43:04: 16000000 INFO @ Sun, 21 Jun 2020 21:43:05: 21000000 INFO @ Sun, 21 Jun 2020 21:43:11: 26000000 INFO @ Sun, 21 Jun 2020 21:43:11: 17000000 INFO @ Sun, 21 Jun 2020 21:43:12: 22000000 INFO @ Sun, 21 Jun 2020 21:43:18: 27000000 INFO @ Sun, 21 Jun 2020 21:43:18: 18000000 INFO @ Sun, 21 Jun 2020 21:43:19: 23000000 INFO @ Sun, 21 Jun 2020 21:43:26: 28000000 INFO @ Sun, 21 Jun 2020 21:43:26: 19000000 INFO @ Sun, 21 Jun 2020 21:43:26: 24000000 INFO @ Sun, 21 Jun 2020 21:43:33: 29000000 INFO @ Sun, 21 Jun 2020 21:43:33: 20000000 INFO @ Sun, 21 Jun 2020 21:43:33: 25000000 INFO @ Sun, 21 Jun 2020 21:43:40: 26000000 INFO @ Sun, 21 Jun 2020 21:43:40: 30000000 INFO @ Sun, 21 Jun 2020 21:43:41: 21000000 INFO @ Sun, 21 Jun 2020 21:43:48: 27000000 INFO @ Sun, 21 Jun 2020 21:43:48: 31000000 INFO @ Sun, 21 Jun 2020 21:43:48: 22000000 INFO @ Sun, 21 Jun 2020 21:43:55: 28000000 INFO @ Sun, 21 Jun 2020 21:43:55: 23000000 INFO @ Sun, 21 Jun 2020 21:43:55: 32000000 INFO @ Sun, 21 Jun 2020 21:44:02: 29000000 INFO @ Sun, 21 Jun 2020 21:44:02: 24000000 INFO @ Sun, 21 Jun 2020 21:44:03: 33000000 INFO @ Sun, 21 Jun 2020 21:44:09: 30000000 INFO @ Sun, 21 Jun 2020 21:44:10: 25000000 INFO @ Sun, 21 Jun 2020 21:44:10: 34000000 INFO @ Sun, 21 Jun 2020 21:44:16: 31000000 INFO @ Sun, 21 Jun 2020 21:44:17: 26000000 INFO @ Sun, 21 Jun 2020 21:44:17: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:44:24: 32000000 INFO @ Sun, 21 Jun 2020 21:44:24: 27000000 INFO @ Sun, 21 Jun 2020 21:44:24: 36000000 INFO @ Sun, 21 Jun 2020 21:44:31: 33000000 INFO @ Sun, 21 Jun 2020 21:44:32: 37000000 INFO @ Sun, 21 Jun 2020 21:44:32: 28000000 INFO @ Sun, 21 Jun 2020 21:44:39: 34000000 INFO @ Sun, 21 Jun 2020 21:44:39: 38000000 INFO @ Sun, 21 Jun 2020 21:44:39: 29000000 INFO @ Sun, 21 Jun 2020 21:44:46: 39000000 INFO @ Sun, 21 Jun 2020 21:44:46: 35000000 INFO @ Sun, 21 Jun 2020 21:44:47: 30000000 INFO @ Sun, 21 Jun 2020 21:44:53: 36000000 INFO @ Sun, 21 Jun 2020 21:44:53: 40000000 INFO @ Sun, 21 Jun 2020 21:44:55: 31000000 INFO @ Sun, 21 Jun 2020 21:45:01: 41000000 INFO @ Sun, 21 Jun 2020 21:45:01: 37000000 INFO @ Sun, 21 Jun 2020 21:45:02: 32000000 INFO @ Sun, 21 Jun 2020 21:45:08: 42000000 INFO @ Sun, 21 Jun 2020 21:45:08: 38000000 INFO @ Sun, 21 Jun 2020 21:45:10: 33000000 INFO @ Sun, 21 Jun 2020 21:45:15: 43000000 INFO @ Sun, 21 Jun 2020 21:45:15: 39000000 INFO @ Sun, 21 Jun 2020 21:45:17: 34000000 INFO @ Sun, 21 Jun 2020 21:45:22: 44000000 INFO @ Sun, 21 Jun 2020 21:45:23: 40000000 INFO @ Sun, 21 Jun 2020 21:45:25: 35000000 INFO @ Sun, 21 Jun 2020 21:45:29: 45000000 INFO @ Sun, 21 Jun 2020 21:45:31: 41000000 INFO @ Sun, 21 Jun 2020 21:45:32: 36000000 INFO @ Sun, 21 Jun 2020 21:45:36: 46000000 INFO @ Sun, 21 Jun 2020 21:45:38: 42000000 INFO @ Sun, 21 Jun 2020 21:45:40: 37000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:45:43: 47000000 INFO @ Sun, 21 Jun 2020 21:45:45: 43000000 INFO @ Sun, 21 Jun 2020 21:45:47: 38000000 INFO @ Sun, 21 Jun 2020 21:45:50: 48000000 INFO @ Sun, 21 Jun 2020 21:45:53: 44000000 INFO @ Sun, 21 Jun 2020 21:45:55: 39000000 INFO @ Sun, 21 Jun 2020 21:45:57: 49000000 INFO @ Sun, 21 Jun 2020 21:46:00: 45000000 INFO @ Sun, 21 Jun 2020 21:46:03: 40000000 INFO @ Sun, 21 Jun 2020 21:46:05: 50000000 INFO @ Sun, 21 Jun 2020 21:46:07: 46000000 INFO @ Sun, 21 Jun 2020 21:46:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:46:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:46:09: #1 total tags in treatment: 50482256 INFO @ Sun, 21 Jun 2020 21:46:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:46:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:46:10: #1 tags after filtering in treatment: 50482215 INFO @ Sun, 21 Jun 2020 21:46:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:46:10: #1 finished! INFO @ Sun, 21 Jun 2020 21:46:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:46:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:10: 41000000 INFO @ Sun, 21 Jun 2020 21:46:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:46:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:46:14: 47000000 INFO @ Sun, 21 Jun 2020 21:46:18: 42000000 INFO @ Sun, 21 Jun 2020 21:46:22: 48000000 INFO @ Sun, 21 Jun 2020 21:46:25: 43000000 INFO @ Sun, 21 Jun 2020 21:46:29: 49000000 INFO @ Sun, 21 Jun 2020 21:46:33: 44000000 INFO @ Sun, 21 Jun 2020 21:46:37: 50000000 INFO @ Sun, 21 Jun 2020 21:46:40: 45000000 INFO @ Sun, 21 Jun 2020 21:46:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:46:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:46:41: #1 total tags in treatment: 50482256 INFO @ Sun, 21 Jun 2020 21:46:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:46:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:46:42: #1 tags after filtering in treatment: 50482215 INFO @ Sun, 21 Jun 2020 21:46:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:46:42: #1 finished! INFO @ Sun, 21 Jun 2020 21:46:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:46:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:45: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:46:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:46:47: 46000000 INFO @ Sun, 21 Jun 2020 21:46:53: 47000000 INFO @ Sun, 21 Jun 2020 21:47:00: 48000000 INFO @ Sun, 21 Jun 2020 21:47:07: 49000000 INFO @ Sun, 21 Jun 2020 21:47:14: 50000000 INFO @ Sun, 21 Jun 2020 21:47:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:47:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:47:17: #1 total tags in treatment: 50482256 INFO @ Sun, 21 Jun 2020 21:47:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:47:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:47:18: #1 tags after filtering in treatment: 50482215 INFO @ Sun, 21 Jun 2020 21:47:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:47:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:47:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:47:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:47:21: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:47:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:47:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4828018/SRX4828018.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling